Molecular identification of species and production origins of edible bird's nest using FINS and SYBR green I based real-time PCR

Quek, Meei Chien; Chin, Nyuk Ling; Tan, Sheau Wei; Yusof, Yus Aniza; Law, Chung Lim

doi:10.1016/j.foodcont.2017.07.027

Cited by 33 publications

(12 citation statements)

References 33 publications

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“…The results found that the apparent fluorescence signal can be observed in all eight Gadiformes species by adding genomic DNA at levels as low as 0.048 ng (Figure S1). This level was comparable to the LODs of previous studies on other authentication assays using SYBR Green real‐time method (Quek et al , ), but represented a less sensitivity compared with probe‐based real‐time PCR (Prado et al , ; Taboada et al , ). Nevertheless, several advantages, such as the simple design, easy set‐up and low cost, still make SYBR Green method a wide application on food fraud detection (Safdar & Junejo, ).…”

Section: Resultssupporting

confidence: 80%

Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR

Xiong

Yuan

Huang

et al. 2019

Int J of Food Sci Tech

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Summary Using species outside the order Gadiformes to prepare codfish products has been widely revealed as a new type of fraud in China, highlighting the necessity to establish proper methods for codfish species identification. This work aimed to develop a new SYBR Green real‐time PCR assay for the detection of codfish ingredient (mainly the eight Gadiformes species, Gadus morhua, Gadus macrocephalus, Theragra chalcogramma, Pollachius virens, Melanogrammus aeglefinus, Merluccius merluccius, Merluccius australis and Albatrossia pectoralis) in processed fish products based on mitochondrial 12S rRNA gene. Results highlighted the positive Cq (cycle quantification value, average value 19.18 ± 1.49) only from the eight Gadiformes species, with no fluorescent signal (Cq > 32) for the other sixty‐seven non‐Gadiformes species. The absolute limit of detection was 0.048 ng genomic DNA. Methodology validation succeeded in twenty‐six commercial products. These results demonstrated that the newly developed method is suitable for the identification of codfish ingredient in processed fish products.

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Section: Resultssupporting

confidence: 80%

Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR

Xiong

Yuan

Huang

et al. 2019

Int J of Food Sci Tech

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“…Various studies have been conducted in detecting adulterants in EBN using different methods with different percentage limit detections. These include polymerase chain reaction (PCR) (Guo et al, 2014;Quek et al, 2018), enzyme-linked immunosorbent assays (ELISAs) (Nur Azira et al, 2016), infrared (IR) spectroscopy (Guo et al, 2014;Shi et al, 2017) and gas chromatography/liquid chromatography-mass spectrometry (GC/LC-MS) (Yang et al, 2014). Fourier transform infrared spectroscopy (FTIR) is one of the applications of mid-IR spectroscopy, which can obtain spectra from solid, liquid or gas samples (Paul & Genesca, 2013).…”

Section: Introductionmentioning

confidence: 99%

Detection of Edible Bird’s Nest Using Fourier Transform Infrared Spectroscopy (FTIR) Combined with Principle Component Analysis (PCA)

Atikah¹,

Tukiran

Azarima³

et al. 2020

JHIS

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Edible bird’s nest (EBN) is rich in nutrients and health benefits; making it one of the Chinese delicacies over the centuries. However, due to the overpriced and limited supply of EBN, it is being adulterated with other cheaper versions. Therefore, the aim of this study is to establish a method of detecting adulterants in EBN using Fourier transform infrared spectroscopy (FTIR) as the spectrum fingerprinting analysis together with principal component analysis (PCA). Spiked samples have been developed for Tremella fungus and porcine gelatine at the concentrations of 1%, 5%, 10%, 20% and 30% (w/w). The FTIR method combined with PCA analysis was able to detect the adulteration of porcine gelatine and Tremella fungus in the sample of adulterated EBNs at low concentration of 1% (w/w). The simple approach employing FTIR combined with PCA may provide a useful tool for EBN detection.

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“…Real‐time PCR assays have a very low limit of detection (LOD), and it is possible to discriminate species even from fragmented DNA . In addition, SYBR Green‐based real‐time PCR assays are less costly than probe‐based assays …”

Section: Introductionmentioning

confidence: 99%

“…20 In addition, SYBR Green-based real-time PCR assays are less costly than probe-based assays. 21 The genus Zanthoxylum (Rutaceae) consists of approximately 250 species worldwide, of which six are distributed in Korea. 22 Among these species, three Zanthoxylum species, Z. schinifolium, Z. piperitum and Z. armatum, are distributed throughout the Korean Peninsula and traditionally have been used for food and medicinal materials.…”

Section: Introductionmentioning

confidence: 99%

Development of conventional PCR and real‐time PCR assays to discriminate the origins of Chinese pepper oil and herbal materials from Zanthoxylum

et al. 2018

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BACKGROUND To ensure the safety, quality and therapeutic efficacy of processed foods and herbal medicines, it is important to identify and discriminate economically motivated adulterants. Zanthoxylum schinifolium is sold at a higher price than other Zanthoxylum species and is frequently adulterated with closely related Zanthoxylum species because of its high demand as a Korean food ingredient and medicinal material in markets. In addition, the pericarps of three Zanthoxylum species ( Z. schinifolium , Z. bungeanum and Z. piperitum ) are defined as herbal medicine Zanthoxyli Pericarpium in Korean pharmacopoeias, but not Z. piperitum in Chinese pharmacopoeias. Further confusion arises in the morphological similarity between Z. armatum (adulterant) and Z. bungeanum . Therefore, the aim of this study was to develop a sequence characterized amplified region (SCAR) marker for discrimination of four Zanthoxylum species. RESULTS With the goal of developing rapid and reliable tools for genetic discrimination of authentic Zanthoxyli Pericarpium, we designed species‐specific SCAR markers, based on ITS2 sequences, that generate amplicons of less than 200 bp. Using these markers, we established both conventional and real‐time PCR assay methods capable of differentiating samples at the species level. We validated the ability of SCAR markers to authenticate edible oil and herbal medicine, and confirmed that some herbal medicines contaminated with Z. armatum are being distributed as Zanthoxyli Pericarpium in Korean and Chinese markets. CONCLUSIONS The SCAR markers and PCR methods described represent powerful tools for protecting against adulteration and ensuring standardization of processed foods and herbal medicine. © 2018 The Authors. Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Molecular identification of species and production origins of edible bird's nest using FINS and SYBR green I based real-time PCR

Cited by 33 publications

References 33 publications

Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR

Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR

Detection of Edible Bird’s Nest Using Fourier Transform Infrared Spectroscopy (FTIR) Combined with Principle Component Analysis (PCA)

Development of conventional PCR and real‐time PCR assays to discriminate the origins of Chinese pepper oil and herbal materials from Zanthoxylum

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