Molecular identification of trypanosomatids in wild animals

Tenório, Michely S.; Sousa, L. Oliveira e; Alves-Martin, Maria Fernanda; Paixão, M. S.; Rodrigues, Marianna Vaz; Starke-Buzetti, Wilma Aparecida; Araújo, João Pessoa; Lucheis, Simone Baldini

doi:10.1016/j.vetpar.2014.02.010

Cited by 20 publications

(14 citation statements)

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“…In addition to two zoonotic trypanosome species, T. cruzi and T. rangeli , neotropical non-human primates, primarily species of the Cebidae family, can also be infected with T. (Megatrypanum) minasense (Chagas, 1908, Dunn et al., 1963, Hoare, 1972, Deane et al., 1974, De Resende et al., 1994, Ziccardi et al., 2000, Sato et al., 2008, Tenório et al., 2014). Regarding the genus Alouatta , trypomastigotes of T. minasense have been recorded by morphologic techniques in wild individuals of Alouatta palliata from Costa Rica (Chinchilla et al., 2005) and by molecular diagnosis in one captive A. caraya in the Centre for Wild Fauna (CCWF) from Brazil (Tenório et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Regarding the genus Alouatta , trypomastigotes of T. minasense have been recorded by morphologic techniques in wild individuals of Alouatta palliata from Costa Rica (Chinchilla et al., 2005) and by molecular diagnosis in one captive A. caraya in the Centre for Wild Fauna (CCWF) from Brazil (Tenório et al., 2014). The highly pleomorphic nature of T. (Megatrypanum) minasense in the bloodstream requires accurate identification of the species based on not only morphology.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Martínez

Kowalewski

Salomón

et al. 2016

International Journal for Parasitology: Parasites and Wildlife

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The transmission of Trypanosoma cruzi by vectors is confined to the Americas, and the infection circulates in at least two broadly defined transmission cycles occurring in domestic and sylvatic habitats. This study sought to detect and characterize infection by T. cruzi and other trypanosomes using PCR strategies in blood samples from free-ranging howler monkeys, Alouatta caraya, in the northeastern Argentina. Blood samples were collected at four sites with variable levels of habitat modification by human activity. PCR was conducted using primers for kinetoplast DNA, satellite DNA and ribosomal DNA of the trypanosomatid parasites. Ribosomal and satellite DNA fragments were sequenced to identify the trypanosomatid species and to characterize the discrete typing units (DTUs) of T. cruzi. Overall, 46% (50/109) of the howlers were positive according to the kDNA-PCR assay, but only 7 of the howlers were positive according to the SatDNA-PCR protocol. We sequenced the amplicons of the satellite DNA obtained from five specimens, and the sequences were 99% and 100% similar to T. cruzi. A sequence typical of DTU T. cruzi I was found in one howler monkey from the “remote” site, while sequences compatible with DTUs II, V, and VI were found in howlers from the “remote”, “rural” and “village” sites. We detected 96% positive samples for RibDNA-PCR, 9 of which were sequenced and displayed 99% identity with Trypanosoma minasense, while none showed identity with T. cruzi. The results demonstrated the presence of T. cruzi and a species closely related to T. minasense in blood samples from free-ranging A. caraya, belonging to different T. cruzi DTUs circulating in these howler monkey populations. The results obtained in this study could help evaluate the role of A. caraya as a reservoir of T. cruzi in regions where Chagas disease is hyper-endemic and where the human-wildlife interface is increasing.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Martínez

Kowalewski

Salomón

et al. 2016

International Journal for Parasitology: Parasites and Wildlife

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“…Even though kDNA still is considered the most sensitive target for the diagnosis of leishmaniasis (Koltas et al, 2016), the internal transcribed spacer 1 (ITS1) ribosomal region has also been used to amplify the DNA of different trypanosomatids (El Tail et al, 2000.;Schönian et al, 2003;Tenório et al, 2014). Thus, we decided to examine these animals using PCR targeting the ITS1, due to its high sensitivity for detecting Leishmania or Trypanosoma species and because this technique generates high-quality fragments for sequencing (Pilatti et al, 2009;Ferreira et al, 2014;Koltas et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Detection of Leishmania infantum DNA in conjunctival swabs of cats by quantitative real-time PCR

Benassi

Benvenga

Ferreira

et al. 2017

Experimental Parasitology

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“…Minervino et al (2010) tested the sera of captive agoutis (D. aguti) from Brazil for T. gondii antibodies using the modified agglutination test (MAT) and found that 18% were positive. Tenório et al (2014) testedfree-ranging agoutis (D. aguti) in Brazil using blood cultures and polymerase chain reaction (PCR) and found that 6.25% was positive for Trypanosoma cruzi (Table 1). Lall, Kegan Romelle Jones and Gary Wayne Garcia (2018) Int.…”

Section: Introductionmentioning

confidence: 99%

Infectious Diseases of Six Non-Domesticated Neo-Tropical Animals In Trinidad and Tobago

Lall¹,

Jones²,

Garcia³

2018

IJTVBR

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This review serves to shed light on some common infectious diseases: their names, prevalence, site of infection and diagnostic tools for identificationof six (6) neo-tropical animals that are found in Trinidad and Tobago with the potential for domestication. These are theagouti(Dasyprocta leporina/ D. agouti), lappe/paca (Cuniculus paca/ Agouti paca), capybara (Hydrochoerus hydrochaeris), manicou/opossum (Didelphis marsupialis insularis), collared peccary (Tayassu tajacu/ Peccari tajucu) and red brocket deer (Mazama americana).There is over Ninety (90) references were used, with the earliest being 1951 and the most recent being in 2017.Where literature was lacking, information from other species within the same genus was incorporated and identified clearly. It was interesting to note that the majority of animals, despite being infected with pathogenic organisms were clinically healthy. In animals that showed clinical signs of disease there was a large quantity of pathogens present. This manuscript sheds some light on the pathogens that may use these animals as natural reservoirs. This review paper also includes many of the earliest identification of these pathogens in Neo-tropical animals in specific geographic regions.

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Molecular identification of trypanosomatids in wild animals

Cited by 20 publications

References 18 publications

Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Molecular characterization of trypanosomatid infections in wild howler monkeys ( Alouatta caraya ) in northeastern Argentina

Detection of Leishmania infantum DNA in conjunctival swabs of cats by quantitative real-time PCR

Infectious Diseases of Six Non-Domesticated Neo-Tropical Animals In Trinidad and Tobago

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