Molecular Identification of Tuber Species and Isolates by PCR-Based Techniques

Identification of ectomycorrhizae from Tuber species by rflp analysis of the its region

“…ITS is a convenient target region for the molecular identification of mycorrhizal fungi in the different phases of their life cycle (Henrion et al 1992, Erland et al 1993, Paolocci et al 1995, Lanfranco et al 1995, Gandebouef et al 1995. In fact, this region is present in all the fungi of interest and is easy to amplify with the "universal primers" that are complementary to sequences within the rRNA genes.…”

“…Some of these techniques have been applied for the correct identification of truffles during the different phases of their life cycle especially when morphological characters are ambiguous or missing (Lanfranco et al 1993, Henrion et al 1994, Potenza et al 1994, Gandebouef et al 1995, Paolocci et al 1995. One efficient strategy is the restriction fragment length polymorphism (RFLP) analysis of the PCR amplified internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA (Gardes and Bruns 1990).…”