Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian Peninsula

Abstract

Random amplified polymorphic DNA (RAPD) analysis has been used to identify the eight Barbus species of the Iberian Peninsula, whose morphological differentiation is difficult. Ten random primers were employed to generate RAPD markers. Different RAPD profiles were observed for the different species. Four species-specific markers were found in B. bocagei, seven in B. comizo, five in B. graellsii, three in B. guiraonis, eight in B. haasi, 13 in B. meridionalis, four in B. microcephalus and four in B. sclateri. Evidence is presented that RAPD markers constitute useful tools for accurate taxonomic identification of Spanish barbels which is one of the first prerequisites in effective conservation programmes. 2001 The Fisheries Society of the British Isles

Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers

“…Bands that were monomorphic in one species (and therefore its diagnostic markers) or some species, but absent in the rest, are those mainly responsible for the greater inter-rather than intraspecific polymorphism detected (Callejas and Ochando, 2001). Levels of intraspecific polymorphism ranged between 0 and 29.36% (Table 2).…”

Molecular Identification of Tropical Tasar Silkworm (Antheraea mylitta) Ecoraces with RAPD and SCAR Markers

“…When considered together, the two marker systems were able to assign individual identity profiles to all 10 ecoraces. These RAPD and SCAR markers may be used for identification of the different populations, establishment of germplasms (Callejas and Ochando, 2001), MAS (molecular assisted selection; Wang et al, 2004), and tracking the evolution of new hybrids (Behura et al, 1999).…”

Molecular Electrophoretic Technique for Authentication of the Fish Genetic Diversity

“…For species identification and genetic resource/diversity analysis, RAPD and microsatellitsa method were recommended (Liu & Cordes, 2004). The RAPD techniques has been used for discrimination of populations of species of the genus Barbus, grouper, Nile perch and wreck fish, salmonids, among others (Partis & Wells, 1996;Callejas & Ochando, 2001;Asensio et al, 2002;Jin, Cho, Seong, Park, Kong & Hong, 2006). Genetic analysis with RAPD markers is relatively easy, fast, and efficient.…”