Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window

Zhu, Shoujun; Yang, Qinglai; Antaris, Alexander L.; Yue, Jingying; Ma, Zhuoran; Wang, Huasen; Huang, Wei; Wan, Hao; Wang, Joy; Diao, Shuo; Zhang, Bo; Li, Xiaoyang; Zhong, Yongwang; Yu, Kuai; Hong, Guosong; Luo, Jian; Liang, Yongye; Dai, Hongjie

doi:10.1073/pnas.1617990114

Cited by 249 publications

(184 citation statements)

References 47 publications

Supporting

Mentioning

181

Contrasting

Unclassified

Order By: Relevance

“…Although our NIR-II fluorescence wide-field microscopy was capable of realizing real-time brain imaging on rhesus macaques, its spatial resolution and SBR were inevitably influenced by out-of-focus signals (fluorescence signals both above and below the focal plane of objective). To address this challenge, we turned to NIR-II fluorescence confocal microscopy, which offers fine optical sectioning and high SBR, as well as large penetration depth from NIR-II fluorescence bioimaging, as demonstrated by ex vivo and in vivo bioimaging in mice 17,36,44,45 . To achieve this capability in large animals, we custom-designed a NIR-II fluorescence confocal microscopic system, modified from our previous lab-built setup 33,41 .…”

Section: Resultsmentioning

confidence: 99%

NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates

Cai

Zhu

Wang

et al. 2019

Preprint

View full text Add to dashboard Cite

Vasculature architecture in the brain can provide revealing information about mental and neurological function and disease. Fluorescence imaging in the second near-infrared (NIR-II) regime with less light scattering is a more promising method for detecting cortical vessels than traditional visible and NIR-I modes. Here, for the first time, we developed, NIR-II fluorescence microscopy capabilities for imaging brain vasculature in macaque monkey. The first is a wide-field microscope with high temporal resolution (25 frames/second) for measuring blood flow velocity and cardiac impulse period, and the second is a high spatial resolution (<10 μm) confocal microscope producing three-dimensional maps of the cortical microvascular network (~500 μm deep). Both were designed with flexibility to image various cortical locations on the head. Use of a clinically approved dye provided high brightness in NIR-II region. This comprises an important advance towards studies of neurovascular coupling, stroke, and other diseases relevant to neurovascular health in humans.

show abstract

Section: Resultsmentioning

confidence: 99%

NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates

Cai

Zhu

Wang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…SWCNTsa rc discharge, laser ablation, and chemical ozone,diazonium, alkyl carboxylation, hydrogen peroxide, phospho- [14], [15], [16], [17] vapor deposition lipid (PL)-polyethylene glycol (PEG) QDs hot injection, solvothermal, microwave core-shellp articles, Pb 2 + -dopedA g 2 SQ Ds, CdS-coated shell to protect [18], [19], [ 20], irradiation the PbS core (PEGylation) [21], [22] RE compounds sol-gel, coprecipitation, hydrothermal core-shell particles (core: hostm aterial anddopants;shell:u ndoped [ 23], [24] host material;installation of 2% Ce and Er codoped NaYbF 4 corea nd an inert NaYF 4 shell) conjugated withP EG-based polymers organic StilleorS uzuki coupling between electron-micellar, PEGylation electron-acceptor unit:B BTD), electron-donor [25], [26], [27], fluorescent acceptor unit andelectron-donor unit,n ano-u nit:t riphenylamine, thiophene,electron-shielding unit:2,6-alkoxy [28], [29], [30], materials precipitation chain substitution, alkyl chain substituted fluorine [31], [32], [33] [a] BBTD: benzo[1,2-c:4,5-c']bis( [1,2,5]thiadiazole. Figure 4.…”

Section: Categorymentioning

confidence: 99%

Recent Progress in Fluorescence Imaging of the Near‐Infrared II Window

Miao

Zhu

et al. 2018

ChemBioChem

View full text Add to dashboard Cite

Near‐infrared (NIR) fluorescent materials are considered to be the most promising labeling reagents for sensitive determination and biological imaging due to the advantages of lower background noise, deeper penetrating capacity, and less destructive effects on the biomatrix over those of UV and visible fluorophores. In the past decade, advances in biomedical fluorescence imaging in the NIR region have focused on the traditional NIR window (NIR‐I; λ=700–900 nm), and have recently been extended to the second NIR window (NIR‐II; λ=1000–1700 nm). In vivo NIR‐II fluorescence imaging outperforms imaging in the NIR‐I window as a result of further reduced absorption, tissue autofluorescence, and scattering. In this review, the applications of four types of NIR‐II fluorescent materials, organic fluorophores, quantum dots, rare‐earth compounds, and single‐walled carbon nanotubes, are summarized and future trends are discussed. Some methods to enhance the NIR‐II fluorescence quantum yield are also proposed.

show abstract

“…Up to now, in most studies, NIR‐II imaging is limited to two‐dimensional (2D) projected wide‐field epi‐fluorescence imaging without the capability of gleaning three‐dimensional (3D) tissue structures deeply. NIR‐II imaging applications are commonly adopted for whole‐body small‐animal imaging experiments performed in wide‐field to visualize vasculatures or organs with relatively low resolution and no optical sectioning capability .…”

Section: Introductionmentioning

confidence: 99%

Three‐dimensional cellular imaging in thick biological tissue with confocal detection of one‐photon fluorescence in the near‐infrared II window

Wang

Chen

2019

Journal of Biophotonics

View full text Add to dashboard Cite

Fluorescence imaging in the second near‐infrared optical window (NIR‐II, 900‐1700 nm) has become a technique of choice for noninvasive in vivo imaging in recent years. Greater penetration depths with high spatial resolution and low background can be achieved with this NIR‐II window, owing to low autofluorescence within this optical range and reduced scattering of long wavelength photons. Here, we present a novel design of confocal laser scanning microscope tailored for imaging in the NIR‐II window. We showcase the outstanding penetration depth of our confocal setup with a series of imaging experiments. HeLa cells labeled with PbS quantum dots with a peak emission wavelength of 1276 nm can be visualized through a 3.5‐mm‐thick layer of scattering medium, which is a 0.8% Lipofundin solution. A commercially available organic dye IR‐1061 (emission peak at 1132 nm), in its native form, is used for the first time, as a NIR‐II fluorescence label in cellular imaging. Our confocal setup is capable of capturing optically sectioned images of IR‐1061 labeled chondrocytes in fixed animal cartilage at a depth up to 800 μm, with a superb spatial resolution of around 2 μm.

show abstract

Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window

Cited by 249 publications

References 47 publications

NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates

NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates

Recent Progress in Fluorescence Imaging of the Near‐Infrared II Window

Three‐dimensional cellular imaging in thick biological tissue with confocal detection of one‐photon fluorescence in the near‐infrared II window

Contact Info

Product

Resources

About