Molecular imaging with targeted contrast ultrasound

Kaufmann, Beat A.; Lindner, Jonathan R.

doi:10.1016/j.copbio.2007.01.004

Cited by 190 publications

(123 citation statements)

References 32 publications

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“…This complication is due to its asymptomatic nature, as vulnerable plaques grow without causing any detrimental side effects until rupture ( 1 ). Current arterial imaging techniques that are being used in the clinical setting provide information only at later stages of the disease process (e.g., stenosis and thrombus formation, by angiography and angioscopy, respectively) or produce limited information about the vessel wall and general tissue structure (e.g., intima-media thickness and presence of lipid-rich tissue, by ultrasound and magnetic resonance imaging, respectively) (4)(5)(6)(7)(8). Although these techniques provide insight into the disease state, they are incapable of cellular-level resolution and typically require the addition of exogenous contrast agents ( 4,(9)(10)(11)(12).…”

Section: Atherosclerotic Apoe-defi Cient Mouse Modelmentioning

confidence: 99%

Multimodal CARS microscopy determination of the impact of diet on macrophage infiltration and lipid accumulation on plaque formation in ApoE-deficient mice

Lim

Kratzer

Barry

et al. 2010

Journal of Lipid Research

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Section: Atherosclerotic Apoe-defi Cient Mouse Modelmentioning

confidence: 99%

Multimodal CARS microscopy determination of the impact of diet on macrophage infiltration and lipid accumulation on plaque formation in ApoE-deficient mice

Lim

Kratzer

Barry

et al. 2010

Journal of Lipid Research

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“…Recently, there has been a growing interest in photoacoustic computed tomography (PCT) imaging (1)(2)(3)(4)(5)(6)(7)(8). It is a noninvasive imaging modality capable for obtaining both structural and functional information in biological tissues.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Evaluation of Near-Infrared (NIR) Dye−Herceptin Conjugates as Photoacoustic Computed Tomography (PCT) Probes for HER2 Expression in Breast Cancer

et al. 2008

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We are evaluating PCT imaging in conjunction with NIR-dye labeled Herceptin antibody for noninvasive assessment of HER2 expression in tumors. Herceptin was labeled with Alexa Fluor-750 amine reactive dye for characterization of photoacoustic and fluorescence signals. Measurements were performed in solution and after incubation in cultured cell lines that were positive or negative in expression of HER2. The dye to antibody ratio was controlled to achieve a broad range of degree of labeling (DOL = 2 to 15). Photoacoustic signal intensity of Herceptin-dye conjugates in solution increased with increasing over the entire DOL range studied. In contrast, fluorescence exhibited significant quenching for higher DOL. In vitro PCT imaging of the labeled HER2 (+) and HER2 (−) cells revealed the targeting specificity of the NIR-dye labeled Herceptin. In HER2 (+) cells lines, photoacoustic signal intensity gradually increased with increasing DOL and with increasing number of cells. These results demonstrate that PCT-based measurement of HER2 receptor binding using NIR-dye labeled Herceptin is feasible. The absence of a quenching effect with increased DOL advantages this method over traditional methods based on fluorescence measurement.

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“…Also in the fi eld of ultrasound, targeted microbubbles or other acoustically active nanoparticles are being used for molecular imaging (16,17) . Microbubbles are injected and targeted to the diseased tissue where they produce a detectable acoustic signal.…”

Section: Vol 4 No 2 2007mentioning

confidence: 99%