Molecular impact of graphene oxide with different shape dimension on human immune cells

Orecchioni, Marco; Jasim, Dhifaf; Pescatori, Mario; Sgarrella, Francesco; Bedognetti, Davide; Bianco, Alberto; Kostarelos, Kostas; Delogu, Lucia Gemma

doi:10.1186/2051-1426-3-s2-p217

Cited by 3 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ma et al showed that l-GO elicited stronger NF-κB activation and pro-inflammatory cytokine expression than s-GO both in vitro and in vivo . However, the lateral size of GO may exert contrasting effects on pro-inflammatory cytokine expression in human immune cells, as s-GO induces stronger expression of cytokines in human peripheral blood mononuclear cells than l-GO . However, in this study, we find that the effects of us-GO, s-GO, and l-GO are comparable with respect to IL-1β and IL-6 production (Figure S2).…”

Section: Discussioncontrasting

confidence: 46%

“…47 However, the lateral size of GO may exert contrasting effects on pro-inflammatory cytokine expression in human immune cells, as s-GO induces stronger expression of cytokines in human peripheral blood mononuclear cells than l-GO. 50 However, in this study, we find that the effects of us-GO, s-GO, and l-GO are comparable with respect to IL-1β and IL-6 production (Figure S2). Higher concentrations of GO also appear to induce NF-κB signaling via TLR4 in RAW264.7 cells and can induce IL-1β release from LPS-primed THP-1 macrophages.…”

Section: Discussionmentioning

confidence: 45%

See 1 more Smart Citation

Small, Thin Graphene Oxide Is Anti-inflammatory Activating Nuclear Factor Erythroid 2-Related Factor 2 via Metabolic Reprogramming

et al. 2018

Self Cite

View full text Add to dashboard Cite

Graphene oxide (GO), an oxidized form of graphene, has potential applications in biomedical research. However, how GO interacts with biological systems, including the innate immune system, is poorly understood. Here, we elucidate the effects of GO sheets on macrophages, identifying distinctive effects of GO on the inflammatory phenotype. Small, thin (s)-GO dose-dependently inhibited release of interleukin (IL)-1β and IL-6 but not tumor necrosis factor α. NLRP3 inflammasome and caspase-1 activation was not affected. The effect of s-GO was pretranslational, as s-GO blocked Toll-like receptor 4-dependent expression of Il1b and Il6 but not Nlrp3 or Tnf mRNA transcripts. s-GO was internalized by immortalized bone-marrow-derived macrophages, suggesting a potential intracellular action. Uptake of polystyrene beads with similar lateral dimensions and surface charge did not phenocopy the effects of s-GO, suggesting that s-GO-mediated inhibition of interleukin expression was not simply due to particle phagocytosis. RNA-Seq analysis established that s-GO had profound effects on the immunometabolism of the cells, leading to activation of the transcription factor nuclear factor erythroid 2-related factor 2, which inhibited expression of cytokines such as IL-1β and IL-6. Thus, we have identified immunometabolic effects of GO that reveal another dimension to its effects on cells. These findings suggest that s-GO may be used as a valuable tool to generate further insights into inflammatory mechanisms and indicate its potential applications in biomedicine.

show abstract

Section: Discussioncontrasting

confidence: 46%

Section: Discussionmentioning

confidence: 45%

Small, Thin Graphene Oxide Is Anti-inflammatory Activating Nuclear Factor Erythroid 2-Related Factor 2 via Metabolic Reprogramming

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Orecchioni et al. 39 also confirmed that different sizes of GO nanosheets would induce different immune system responses. They had analyzed the 84 immune-response-related genes after coincubation with GO-small and GO-large nanosheets, and had known that GO-small regulated more genes than that of GO-large, which clearly showed a size-dependent impact on the immune system.…”

Section: Nano-bio Interactions Of 2d Nanomaterialsmentioning

confidence: 89%

Nano-bio interfaces effect of two-dimensional nanomaterials and their applications in cancer immunotherapy

Tang

Xiao

Kong

et al. 2021

Acta Pharmaceutica Sinica B

View full text Add to dashboard Cite

The field of two-dimensional (2D) nanomaterial-based cancer immunotherapy combines research from multiple subdisciplines of material science, nano-chemistry, in particular nano-biological interactions, immunology, and medicinal chemistry. Most importantly, the “biological identity” of nanomaterials governed by bio-molecular corona in terms of bimolecular types, relative abundance, and conformation at the nanomaterial surface is now believed to influence blood circulation time, bio-distribution, immune response, cellular uptake, and intracellular trafficking. A better understanding of nano-bio interactions can improve utilization of 2D nano-architectures for cancer immunotherapy and immunotheranostics, allowing them to be adapted or modified to treat other immune dysregulation syndromes including autoimmune diseases or inflammation, infection, tissue regeneration, and transplantation. The manuscript reviews the biological interactions and immunotherapeutic applications of 2D nanomaterials, including understanding their interactions with biological molecules of the immune system, summarizes and prospects the applications of 2D nanomaterials in cancer immunotherapy.

show abstract

Nanoparticles Advancing Cancer Immunotherapy

Chowdhury

Kubra

Kanwar

et al. 2019

Biomedical Applications of Graphene and 2D Nanomaterials

View full text Add to dashboard Cite

Molecular impact of graphene oxide with different shape dimension on human immune cells

Cited by 3 publications

References 3 publications

Small, Thin Graphene Oxide Is Anti-inflammatory Activating Nuclear Factor Erythroid 2-Related Factor 2 via Metabolic Reprogramming

Small, Thin Graphene Oxide Is Anti-inflammatory Activating Nuclear Factor Erythroid 2-Related Factor 2 via Metabolic Reprogramming

Nano-bio interfaces effect of two-dimensional nanomaterials and their applications in cancer immunotherapy

Nanoparticles Advancing Cancer Immunotherapy

Contact Info

Product

Resources

About