Molecular influenza surveillance at a tertiary university hospital during four consecutive seasons (2012–2016) in Catalonia, Spain

Andrés, Cristina; Peremiquel-Trillas, Paula; Gimferrer, Laura; Piñana, María; Codina, María Gema; Pendás, J.A. Rodrigo; Campins-Martí, Magda; Martín, Maria Carmen; Fuentes, Francisco; Rubio, Susana; Pumarola, Tomás; Antón, Andrés

doi:10.1016/j.vaccine.2019.03.046

Cited by 5 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the seasonal influenza viruses, A(H3N2) have been characterised by the highest rate of evolution and significant genetic diversity [26][27][28]. Since 2009, seven genetic groups and multiple clades/subclades based on the HA gene have been defined within this subtype.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterisation of the influenza viruses circulating in Bulgaria during the 2019–2020 winter season

et al. 2021

View full text Add to dashboard Cite

Influenza viruses have a high potential for genetic changes. The objectives of this study were to analyse influenza virus circulation in Bulgaria during the 2019/2020 season, to perform a phylogenetic and molecular analyses of the haemagglutinin (HA) and neuraminidase (NA) sequences of representative influenza strains, and to identify amino acid substitutions compared to the current vaccine strains. Seasonal influenza viruses A(H3N2), A(H1N1)pdm09 and B/Victoria-lineage were detected using a real-time RT-PCR in 323 (23.3%), 149 (10.7%) and 138 (9.9%) out of 1387 patient samples studied, respectively. The HA genes of A(H3N2) viruses analysed belonged to clades 3C.3a (21 strains) and 3C.2a (5 strains): subclades 3C.2a1b + T131K, 3C.2a1b + T135K-B and 3C.2a1b + T135K-A. The clade 3C.3a and subclade 3C.2a1b viruses carried 5 and 14–17 substitutions in HA, as well as 3 and 9 substitutions in NA, respectively, in comparison with the A/Kansas/14/2017 vaccine virus, including some substitutions in the HA antigenic sites A, B, C and E. All 21 A(H1N1)pdm09 viruses sequenced fell into 6B.1A5A subclade. Amino acid sequence analysis revealed the presence of 7–11 substitutions in HA, compared to the A/Brisbane/02/2018 vaccine virus, three of which occurred in antigenic site Sb, along with 6–9 changes at positions in NA. All 10 B/Victoria-lineage viruses sequenced belonged to clade 1A with a triple deletion in HA1 (genetic group 1A(Δ3)B) and carried 7 and 3 substitutions in HA and NA, respectively, with respect to the B/Colorado/06/2017 vaccine virus. The results of this study confirm the rapid evolution of influenza viruses and the need for continuous antigenic and genetic surveillance.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic characterisation of the influenza viruses circulating in Bulgaria during the 2019–2020 winter season

et al. 2021

View full text Add to dashboard Cite

show abstract

“…D222G/N polymorphism has been reported in recently circulating viruses [42,43] and two of the recent Bulgarian viruses (A/Bulgaria/013/2019 and A/Bulgaria/043/2019) carried D222N substitution. The D222G substitution was shown to cause a shift from α2,6sialic acid receptor specificity to mixed α2,3/α2,6-sialic acid receptor specificity, adduced thereby to facilitate lung infection [44,45].…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of influenza A viruses circulating in Bulgaria during the 2018–2019 winter season

Korsun

Daniels

Angelova

et al. 2020

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. Influenza viruses evolve rapidly and change their antigenic characteristics, necessitating biannual updates of flu vaccines. Aim. The aim of this study was to characterize influenza viruses circulating in Bulgaria during the 2018/2019 season and to identify amino acid substitutions in them that might impact vaccine effectiveness. Methodology. Typing/subtyping of influenza viruses were performed using real-time Reverse Transcription-PCR (RT-PCR) and results of phylogenetic and amino acid sequence analyses of influenza strains are presented. Results. A(H1N1)pdm09 (66 %) predominated over A(H3N2) (34 %) viruses, with undetected circulation of B viruses in the 2018/2019 season. All A(H1N1)pdm09 viruses studied fell into the recently designated 6B.1A subclade with over 50 % falling in four subgroups: 6B.1A2, 6B.1A5, 6B.1A6 and 6B.1A7. Analysed A(H3N2) viruses belonged to subclades 3C.2a1b and 3C.2a2. Amino acid sequence analysis of 36 A(H1N1)pdm09 isolates revealed the presence of six–ten substitutions in haemagglutinin (HA), compared to the A/Michigan/45/2015 vaccine virus, three of which occurred in antigenic sites Sa and Cb, together with four–nine changes at positions in neuraminidase (NA), and a number of substitutions in internal proteins. HA1 D222N substitution, associated with increased virulence, was identified in two A(H1N1)pdm09 viruses. Despite the presence of several amino acid substitutions, A(H1N1)pdm09 viruses remained antigenically similar to the vaccine virus. The 28 A(H3N2) viruses characterized carried substitutions in HA, including some in antigenic sites A, B, C and E, in NA and internal protein sequences. Conclusion. The results of this study showed the genetic diversity of circulating influenza viruses and the need for continuous antigenic and molecular surveillance.

show abstract

“…In Spain, a surveillance study in Catalonia with samples from 2006 to 2012 reports that no resistant viruses were found, except for the seasonal A H1N1 virus, which practically disappeared with the emergence of the A H1N1 pandemic in 2009. In the same region, has been reported that between 2015 and 2016 only three viruses with resistance markers were detected among 1223 samples analyzed (60).…”

Section: Drug Resistance In Influenza Virusesmentioning

confidence: 93%

Antiviral resistance in influenza viruses

Luis Márquez Domínguez,

Kevin Márquez Matla,

Julio Reyes Leyva

et al. 2023

Cell Mol Biol (Noisy-le-grand)

View full text Add to dashboard Cite

Molecular influenza surveillance at a tertiary university hospital during four consecutive seasons (2012–2016) in Catalonia, Spain

Cited by 5 publications

References 27 publications

Genetic characterisation of the influenza viruses circulating in Bulgaria during the 2019–2020 winter season

Genetic characterisation of the influenza viruses circulating in Bulgaria during the 2019–2020 winter season

Genetic diversity of influenza A viruses circulating in Bulgaria during the 2018–2019 winter season

Antiviral resistance in influenza viruses

Contact Info

Product

Resources

About