Molecular inter-kingdom interactions of endophytes isolated from Lychnophora ericoides

Caraballo‐Rodríguez, Andrés Mauricio; Dorrestein, Pieter C.; Pupo, Mônica Tallarico

doi:10.1038/s41598-017-05532-5

Cited by 21 publications

(17 citation statements)

References 84 publications

(95 reference statements)

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“…The most direct benefit from artificial microbial consortia is the products, either newly synthesized or overexpressed. Metabolites produced from natural or artificial interkingdom communities can be achieved using high‐performance liquid chromatography (HPLC), liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) or nuclear magnetic resonance spectroscopy (NMR) (Caraballo‐Rodríguez et al ., ). Moreover, mass spectrometry imaging (MSI) is an emerging technique, including secondary ion mass spectrometry (SIMS) (Sheik et al ., ; Paine et al ., ) and Raman scattering microspectroscopy (Bodelon et al ., ; Wang et al ., ), which enables the visualization of metabolites in complex samples, such as environmental mixtures and microbial consortia.…”

Section: Potential Methods To Control the Interkingdom Interactionsmentioning

confidence: 97%

Interkingdom microbial consortia mechanisms to guide biotechnological applications

Shu

Merino

Okamoto

et al. 2018

Microbial Biotechnology

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SummaryMicrobial consortia are capable of surviving diverse conditions through the formation of synergistic population‐level structures, such as stromatolites, microbial mats and biofilms. Biotechnological applications are poised to capitalize on these unique interactions. However, current artificial co‐cultures constructed for societal benefits, including biosynthesis, agriculture and bioremediation, face many challenges to perform as well as natural consortia. Interkingdom microbial consortia tend to be more robust and have higher productivity compared with monocultures and intrakingdom consortia, but the control and design of these diverse artificial consortia have received limited attention. Further, feasible research techniques and instrumentation for comprehensive mechanistic insights have only recently been established for interkingdom microbial communities. Here, we review these recent advances in technology and our current understanding of microbial interaction mechanisms involved in sustaining or developing interkingdom consortia for biotechnological applications. Some of the interactions among members from different kingdoms follow similar mechanisms observed for intrakingdom microbial consortia. However, unique interactions in interkingdom consortia, including endosymbiosis or interkingdom‐specific cell–cell interactions, provide improved mitigation to external stresses and inhibitory compounds. Furthermore, antagonistic interactions among interkingdom species can promote fitness, diversification and adaptation, along with the production of beneficial metabolites and enzymes for society. Lastly, we shed light on future research directions to develop study methods at the level of metabolites, genes and meta‐omics. These potential research methods could lead to the control and utilization of highly diverse microbial communities.

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Section: Potential Methods To Control the Interkingdom Interactionsmentioning

confidence: 97%

Interkingdom microbial consortia mechanisms to guide biotechnological applications

Shu

Merino

Okamoto

et al. 2018

Microbial Biotechnology

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“…Caraballo-Rodríguez [5] tested the endophytic actinobacteria Streptomyces cattleya RLe1, S. mobaraensis RLe3, S. albospinus RLe7, Streptomyces sp. RLe9 and Kytasatospora cystarginea RLe10 co-cultured with endophytic fungi Coniochaeta sp.…”

Section: Actinobacteria and Natural Antimicrobial Productsmentioning

confidence: 99%

“…However, several decades passed without significant innovations until the discovery and development of oxazolidinones in 2010 (Figure 1). Moreover, the continuous uncontrolled use of these medicines favored the rapid spread of resistant pathogens, where new compounds were discovered, and their introduction into clinical practice was not fast enough [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Plant-Associated Microorganisms as a Potent Bio-Factory of Active Molecules against Multiresistant Pathogens

Cruz¹,

Bogas²,

Sousa³

2021

Antimicrobial Resistance - A One Health Perspective

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Antibiotic-resistant pathogens are a public health threat that has rapidly spread over decades due to continuous and uncontrolled administration of antimicrobial medicines, becoming an ever-increasing worldwide concern. Since the past decade, no significant innovations have been made, so the search for new compounds that face multidrug-resistant pathogens is critically important. Plant-symbiont microorganisms are capable of producing a variety of bioactive natural products, making it possible to treat several infectious diseases. Biotechnological processes using microorganisms have been increasing in recent years since the discovery of Paclitaxel, an important antimitotic produced by the endophyte Taxomyces andreanae. It was isolated for the first time from the native tree of Pacific Taxus brevifolia. Several studies have demonstrated the isolation and characterization of promising and potent substances capable of inhibiting these pathogens. In addition, both rhizospheric and endophytic communities represent an unexplored reserve of unique chemical structures for drug development. This chapter focuses on the potential of plant-derived microorganisms as a source of bioactive substances and the perspectives for further studies and their application.

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“…Generally, host plants provide an environment with high nutrient content to their microbial inhabitants and shelter them from adverse environmental factors (Compant et al, 2010; Wang M. et al, 2016). Thus, endophytes experience high selective pressure competing for their beneficial, but spatially limited habitat (Caraballo-Rodríguez et al, 2017). Within the endosphere, different plant tissues are characterized by their specific chemoprofiles and tissue architecture, which can act as “filters” and attract different subsets of the endophyte community.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies on Actinobacteria in medicinal plants were conducted in Asia, where the use of herbal remedies is deeply anchored in culture and medicine since millennia, e.g., Ayurveda, Traditional Chinese Medicine (Qin et al, 2009; Zhao et al, 2012). Few studies are available on medicinal plants native to South America, Australia, and the Paleotropics (Golinska et al, 2015; Caraballo-Rodríguez et al, 2017), however, investigations on temperate medicinal herbs are yet underrepresented. Among these, studies on alpine medicinal plants are especially rare.…”

Section: Introductionmentioning

confidence: 99%

Exploring Actinobacteria Associated With Rhizosphere and Endosphere of the Native Alpine Medicinal Plant Leontopodium nivale Subspecies alpinum

et al. 2019

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The rhizosphere of plants is enriched in nutrients facilitating growth of microorganisms, some of which are recruited as endophytes. Endophytes, especially Actinobacteria, are known to produce a plethora of bioactive compounds. We hypothesized that Leontopodium nivale subsp. alpinum (Edelweiss), a rare alpine medicinal plant, may serve as yet untapped source for uncommon Actinobacteria associated with this plant. Rhizosphere soil of native Alpine plants was used, after physical and chemical pre-treatments, for isolating Actinobacteria. Isolates were selected based on morphology and identified by 16S rRNA gene-based barcoding. Resulting 77 Actinobacteria isolates represented the genera Actinokineospora, Kitasatospora, Asanoa, Microbacterium, Micromonospora, Micrococcus, Mycobacterium, Nocardia, and Streptomyces. In parallel, Edelweiss plants from the same location were surface-sterilized, separated into leaves, roots, rhizomes, and inflorescence and pooled within tissues before genomic DNA extraction. Metagenomic 16S rRNA gene amplicons confirmed large numbers of actinobacterial operational taxonomic units (OTUs) descending in diversity from roots to rhizomes, leaves and inflorescences. These metagenomic data, when queried with isolate sequences, revealed an overlap between the two datasets, suggesting recruitment of soil bacteria by the plant. Moreover, this study uncovered a profound diversity of uncultured Actinobacteria from Rubrobacteridae, Thermoleophilales, Acidimicrobiales and unclassified Actinobacteria specifically in belowground tissues, which may be exploited by a targeted isolation approach in the future.

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Molecular inter-kingdom interactions of endophytes isolated from Lychnophora ericoides

Cited by 21 publications

References 84 publications

Interkingdom microbial consortia mechanisms to guide biotechnological applications

Interkingdom microbial consortia mechanisms to guide biotechnological applications

Plant-Associated Microorganisms as a Potent Bio-Factory of Active Molecules against Multiresistant Pathogens

Exploring Actinobacteria Associated With Rhizosphere and Endosphere of the Native Alpine Medicinal Plant Leontopodium nivale Subspecies alpinum

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