Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105

Bradyrhizobium sp. strain SUTN9‐2 was confirmed as rice endophytic bacteria and also as rice growth promotion agent. SUTN9‐2 showed the capability of plant growth promotion characteristics, such as indole‐3‐acetic acid (IAA) and 1‐amino‐cyclopropane‐1‐carboxylic acid (ACC) deaminase productions and nitrogen fixation. In this study, the ability of SUTN9‐2 to stimulate rice growth was investigated at different stages with N‐free and NH4NO3 under in vivo condition. The rice dry weight and chlorophyll content could be enhanced when SUTN9‐2 was inoculated in N‐free, especially at seedling stage (7 and 14 dai). The rice dry weight was also increased when SUTN9‐2 was inoculated with NH4NO3 at 7 and14 dai. The results of quantitative analysis of IAA and ACC deaminase were inconsistent with the expression of genes involved in IAA (nit) and ACC deaminase (acdS) productions. This inconsistently could implied that IAA and ACC deaminase produced from SUTN9‐2 do not directly affect rice growth, but other factors resulting from the production of IAA and ACC deaminase could be involved. Moreover, the expression of genes involved in nitrogen fixation (nifH and nifV) of SUTN9‐2 was also induced in rice tissues. This finding suggested that rice growth promotion may be supported by NH4NO3 together with nitrogen fixation by SUTN9‐2. Significance and Impact of the Study Indole‐3‐acetic acid, 1‐amino‐cyclopropane‐1‐carboxylic acid deaminase productions and nitrogen fixation may play important roles in rice growth promotion by endophytic SUTN9‐2, especially at early rice seedling growth stage, which has the potential to be used as rice seedling growth promoter in the system of rice intensification.

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“…Jaemsaeng et al . () reported that endophytic Streptomyces sp. GMKU 336 enhances growth of rice ( Oryza sativa L. cv.…”

Section: Resultsmentioning

confidence: 99%

“…which was used as a sole nitrogen source (Glick 2014). Jaemsaeng et al (2018) reported that endophytic Streptomyces sp. GMKU 336 enhances growth of rice (Oryza sativa L. cv.…”

Section: Expression Of Nit and Acds Genesmentioning

confidence: 99%

Empowering rice seedling growth by endophytic Bradyrhizobium sp. SUTN 9‐2

Greetatorn

Hashimoto

Sarapat

et al. 2019

Lett Appl Microbiol

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“…In this context, advanced molecular tools and technologies will facilitate the characterization of plant-microbe interactions and may expand our understanding. Protein and gene transcript studies can provide meaningful insights for describing the interactions of plants with beneficial microbes under stress conditions (Cheng et al, 2012;Singh et al, 2017;Jaemsaeng et al, 2018;Kumar et al, 2019). Investigating and analyzing the plant's root protein can provide a clear picture of the changes occurring at the time of microbe interaction.…”

Section: Introductionmentioning

confidence: 99%

Sphingobacterium sp. BHU-AV3 Induces Salt Tolerance in Tomato by Enhancing Antioxidant Activities and Energy Metabolism

Vaishnav

Singh

et al. 2020

Front. Microbiol.

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Salt tolerant bacteria can be helpful in improving a plant's tolerance to salinity. Although plant-bacteria interactions in response to salt stress have been characterized, the precise molecular mechanisms by which bacterial inoculation alleviates salt stress in plants are still poorly explored. In the present study, we aimed to determine the role of a salt-tolerant plant growth-promoting rhizobacteria (PGPR) Sphingobacterium BHU-AV3 for improving salt tolerance in tomato through investigating the physiological responses of tomato roots and leaves under salinity stress. Tomato plants inoculated with BHU-AV3 and challenged with 200 mM NaCl exhibited less senescence, positively correlated with the maintenance of ion balance, lowered reactive oxygen species (ROS), and increased proline content compared to the non-inoculated plants. BHU-AV3-inoculated plant leaves were less affected by oxidative stress, as evident from a reduction in superoxide contents, cell death, and lipid peroxidation. The reduction in ROS level was associated with the increased antioxidant enzyme activities along with multiple-isoform expression [peroxidase (POD), polyphenol oxidase (PPO), and superoxide dismutase (SOD)] in plant roots. Additionally, BHU-AV3 inoculation induced the expression of proteins involved in (i) energy production [ATP synthase], (ii) carbohydrate metabolism (enolase), (iii) thiamine biosynthesis protein, (iv) translation protein (elongation factor 1 alpha), and the antioxidant defense system (catalase) in tomato roots. These findings have provided insight into the molecular mechanisms of bacteria-mediated alleviation of salt stress in plants. From the study, we can conclude that BHU-AV3 inoculation effectively induces antioxidant systems and energy metabolism in tomato roots, which leads to whole plant protection during salt stress through induced systemic tolerance.

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“…We used 3,3′‐diaminobenzidine (DAB) staining to compare H 2 O 2 accumulation in the roots of V. radiata cv. CN72 inoculated with single bradyrhizobial strains (SUTN9–2 or DOA9) and co‐inoculation (SUTN9‐2:DOA9) (Figure A7; Jaemsaeng, Jantasuriyarat, & Thamchaipenet, ). The quantitative H 2 O 2 production was also measured in V. radiata CN72 roots using optical density (OD) measurement (compared with standard H 2 O 2 ; Yasuda et al, ).…”

Section: Resultsmentioning

confidence: 99%

Mutualistic co‐evolution of T3SSs during the establishment of symbiotic relationships between Vigna radiata and Bradyrhizobia

et al. 2019

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This study supports the idea that the evolution of type III secretion system (T3SS) is one of the factors that controls Vigna radiata –bradyrhizobia symbiosis. Based on phylogenetic tree data and gene arrangements, it seems that the T3SSs of the Thai bradyrhizobial strains SUTN9‐2, DOA1, and DOA9 and the Senegalese strain ORS3257 may share the same origin. Therefore, strains SUTN9‐2, DOA1, DOA9, and ORS3257 may have evolved their T3SSs independently from other bradyrhizobia, depending on biological and/or geological events. For functional analyses, the rhcJ genes of ORS3257, SUTN9‐2, DOA9, and USDA110 were disrupted. These mutations had cultivar‐specific effects on nodulation properties. The T3SSs of ORS3257 and DOA9 showed negative effects on V. radiata nodulation, while the T3SS of SUTN9‐2 showed no effect on V. radiata symbiosis. In the roots of V. radiata CN72, the expression levels of the PR1 gene after inoculation with ORS3257 and DOA9 were significantly higher than those after inoculation with ORS3257 ΩT3SS , DOA9 ΩT3SS, and SUTN9‐2. The T3Es from ORS3257 and DOA9 could trigger PR1 expression, which ultimately leads to abort nodulation. In contrast, the T3E from SUTN9‐2 reduced PR1 expression. It seems that the mutualistic relationship between SUTN9‐2 and V. radiata may have led to the selection of the most well‐adapted combination of T3SS and symbiotic bradyrhizobial genotype.

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Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105

Cited by 117 publications

References 73 publications

Empowering rice seedling growth by endophytic Bradyrhizobium sp. SUTN 9‐2

Empowering rice seedling growth by endophytic Bradyrhizobium sp. SUTN 9‐2

Sphingobacterium sp. BHU-AV3 Induces Salt Tolerance in Tomato by Enhancing Antioxidant Activities and Energy Metabolism

Mutualistic co‐evolution of T3SSs during the establishment of symbiotic relationships between Vigna radiata and Bradyrhizobia

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