Molecular Interactions of Proteins and Peptides at Interfaces Studied by Sum Frequency Generation Vibrational Spectroscopy

Liu, Yuwei; Jasensky, Joshua; Chen, Zhan

doi:10.1021/la203823t

Cited by 65 publications

(72 citation statements)

References 94 publications

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“…SFG can probe surfaces or interfaces which are accessible by visible and IR light in situ and in real time with sub-monolayer sensitivity. 40, 91 SFG signals characterize the molecular vibrational modes of functional groups at interfaces, which can be used to probe the interfacial presence, coverage, and orientation of various functional groups. SFG can be applied to investigate interfacial structures at a molecular level even with very low interfacial coverage.…”

Section: Introduction Of Ir + Visible Sum Frequency Generation (Sfmentioning

confidence: 99%

Elucidation of molecular structures at buried polymer interfaces and biological interfaces using sum frequency generation vibrational spectroscopy

2013

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Sum frequency generation (SFG) vibrational spectroscopy has been developed into an important technique to study surfaces and interfaces. It can probe buried interfaces in situ and provide molecular level structural information such as the presence of various chemical moieties, quantitative molecular functional group orientation, and time dependent kinetics or dynamics at such interfaces. This paper focuses on these three most important advantages of SFG and reviews some of the recent progress in SFG studies on interfaces related to polymer materials and biomolecules. The results discussed here demonstrate that SFG can provide important molecular structural information of buried interfaces in situ and in real time, which is difficult to obtain by other surface sensitive analytical techniques.

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Section: Introduction Of Ir + Visible Sum Frequency Generation (Sfmentioning

confidence: 99%

Elucidation of molecular structures at buried polymer interfaces and biological interfaces using sum frequency generation vibrational spectroscopy

2013

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“…14-18 SFG is a surface sensitive second-order nonlinear optical technique and is capable of investigating interfacial structures of peptides and proteins in real time in situ . 19-25 SFG experiment only requires a very small amount of peptide and protein samples. 26-31 …”

Section: Introductionmentioning

confidence: 99%

Lipid Fluid–Gel Phase Transition Induced Alamethicin Orientational Change Probed by Sum Frequency Generation Vibrational Spectroscopy

Yang

Chen

2013

J. Phys. Chem. C

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Alamethicin has been extensively studied as an antimicrobial peptide (AMP) and is widely used as a simple model for ion channel proteins. It has been shown that the antimicrobial activity of AMPs is related to their cell membrane orientation, which may be influenced by the phase of the lipid molecules in the cell membrane. The “healthy” cell membranes contain fluid phase lipids, while gel phase lipids can be found in injured or aged cells or in some phase separated membrane regions. Thus, investigations on how the phase of the lipids influences the membrane orientation of AMPs are important to understand more details regarding the AMP’s action on cell membranes. In this study, we determined the orientational changes of alamethicin molecules associated with planar substrate supported single lipid bilayers (serving as model cell membranes) with different phases (fluid or gel) as a function of peptide concentration using sum frequency generation (SFG) vibrational spectroscopy. The phase changes of the lipid bilayers were realized by varying the sample temperature. Our SFG results indicated that alamethicin lies down on the surface of fluid and gel phase 1,2-dimyristoyl(d54)-sn-glycero-3-phosphocholine (d-DMPC) lipid bilayers when the lipid bilayers are in contact with a peptide solution with a low concentration of 0.84 μM. However, at a medium peptide concentration of 10.80 μM, alamethicin inserts into the fluid phase lipid bilayer. Its orientation switches from a transmembrane to an in-plane (or lying down) orientation when the phase of the lipid bilayer changes from a fluid state to a gel state. At a high peptide concentration of 21.60 μM, alamethicin adopts a transmembrane orientation while associated with both fluid and gel phase lipid bilayers. We also studied the structural changes of the fluid and gel phase lipid bilayers upon their interactions with alamethicin molecules at different peptide concentrations.

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“…It has been demonstrated that SFG vibrational spectroscopy is capable of probing interfacial structures of a variety of polymer and biological surfaces and interfaces [53][54][55]168].…”

Section: Sfg: a Tool For The Biological Surface And Interfacementioning

confidence: 99%

Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors

Zhang

Han

et al. 2013

Chin. Sci. Bull.

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Research in biology and medicine is a rapidly expanding field incorporating some of the most fundamental questions concerning structure, function, and purpose. The forefront of new research demands access to advanced techniques and instrumentation capable of probing these unanswered questions. Over the past several decades, nano-scale materials and devices ranging from quasione dimensional quantum dots to two dimensional graphene sheets have been engineered and have found applications in nano-bio imaging and spectroscopy. In this review, the incorporation of nanomaterials into three influential spectroscopic and microscopic techniques including fluorescence microscopy, surface plasmon resonance, and sum frequency generation will be introduced. Fluorescence imaging has visualized nanomaterials as compliments or replacements to comparable organic fluorphores, act as a quencher for FRET-based sensing, and serve as a nanoscaffold for molecular beacons. Their versatility in coating materials makes nanomaterials an excellent targeting molecule for any cellular macromolecule or structure. In addition to the targeting capabilities of nanomaterials in fluorescence imaging, surface plasmon resonance has incorporated nanomaterials for applications in signal enhancement, selectivity of target molecules, and the development of more refined and accurate detection. Functionalized nanoparticles enhance the capabilities of sum frequency generation vibrational spectroscopy by providing unique surface chemistry which alters target molecule interactions and orientations. In summary, the incorporation of nanomaterials has greatly enhanced the field of biology and medicine and has allowed for the continual advancement of not only research but instrument development. nano-bio interfaces, optical spectroscopy, fluoroscence, SERS, SFG Citation:Zhang X X, Han X F, Wu F G, et al. Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors. Chin Sci Bull, 2013, 58: 25372556, doi: 10.1007/s11434-013-5700-y Nanomaterials, which size ranges (1-100 nm) coincide with fundamentally important length scales in physics, are considered to be promising building blocks for future electrical, optical and optoelectronic applications [1][2][3][4][5][6][7][8]. For example, in metals, the mean free path of an electron at room temperature is ~10-100 nm [9]; the Bohr radius of photo-excited electron-hole pairs in semiconductors is ~1-10 nm [10]; and the 1-100 nm dimension is the range over which molecules assemble into nucleic acids and proteins [11]. Nanostructures on this size scale provide a unique technology to determine many critical structure/function relationships of biological molecules at the cellular level without introducing substantial interference. Advances in nanotechnology have developed at several stages: materials, devices, and systems. A decade ago, extensive research has focused on the development of various methods to precisely control the synthesis of nanomaterials. Therefore, many differ...

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Molecular Interactions of Proteins and Peptides at Interfaces Studied by Sum Frequency Generation Vibrational Spectroscopy

Cited by 65 publications

References 94 publications

Elucidation of molecular structures at buried polymer interfaces and biological interfaces using sum frequency generation vibrational spectroscopy

Elucidation of molecular structures at buried polymer interfaces and biological interfaces using sum frequency generation vibrational spectroscopy

Lipid Fluid–Gel Phase Transition Induced Alamethicin Orientational Change Probed by Sum Frequency Generation Vibrational Spectroscopy

Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors

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