Molecular investigation of extended-spectrum beta-lactamase genes and potential drug resistance in clinical isolates of<i>Morganella morganii</i>

Al-Muhanna, A. S.; Al-Muhanna, Sddiq Ghani; Alzuhairi, Maytham Abbas

doi:10.5144/0256-4947.2016.223

Cited by 15 publications

(7 citation statements)

References 30 publications

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“…It works by interacting with the 30s ribosomal subunit of bacteria, triggering mRNA misreading and preventing the bacterium from synthesizing important proteins for development. This finding agrees with (24), who found that (88.2%) of insulates were resistant to pefloxacin and (64.7%) of isolates were resistant to ciprofloxacin. (76.5%) of insulates were impedance to trimethoprim-sulfamethoxazole, while (94.1%) were impedance to minocycline, (25) observed that all insulates were impedance to both colistin and rifampicin.…”

Section: Antibiogram Testing and Minimum Inhibitory Concentrationsupporting

confidence: 92%

Molecular Exploring of Plasmid-mediated Ampc beta Lactamase Gene in Clinical Isolates of Proteus mirabilis

Al-Kraety

Al-Muhanna

Banoon

2021

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Between September to December 2020, thirteen isolates of Proteus mirabilis were recovered among one hundred fifty; MacConkey agar was utilized to purify Gram-negative bacteria isolated from infections of the urinary tract. The primary identification of Proteus mirabilis isolates was relied on “colonial morphology, microscopic examination, and biochemical “tests; however, the confirmation of identification of antimicrobial susceptibility of isolates was conducted utilizing an automated VITEK-2 compact system. The result showed that Proteus mirabilis isolates were highly resistant to most antibiotics, making them multi-drug resistant (MDR). Phenotype methods were used to detect AmpC beta-lactamase. Initial and confirmatory methods showed that eight isolates were AmpC producers. Polymerase Chain Reaction (PCR) was employed to detect the blaampC gene.

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Section: Antibiogram Testing and Minimum Inhibitory Concentrationsupporting

confidence: 92%

Molecular Exploring of Plasmid-mediated Ampc beta Lactamase Gene in Clinical Isolates of Proteus mirabilis

Al-Kraety

Al-Muhanna

Banoon

2021

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“…In Morganella morganii , the prevalence of the three ESBL genes was relatively high with the TEM gene being the most prevalent. Al-Muhanna, Al-Muhanna & Alzuhari (2016) also showed that all Morganella morganii isolates tested for the ESBL had all the genes. Morganella morganii produces an inducible, chromosomally encoded AmpC β-lactamse, which can be implicated for its natural resistance to aminoopenicillins, amoxicillin-clavulanate, first and second generation cephalosporins with aztreonam, carbenicillin, and tazobactam being effective transient inactivators of some variants ( Power et al, 2006 ).…”

Section: Discussionmentioning

confidence: 88%

Prevalence of extended spectrum beta lactamase and plasmid mediated quinolone resistant genes in strains of Klebsiella pneumonia, Morganella morganii, Leclercia adecarboxylata and Citrobacter freundii isolated from poultry in South Western Nigeria

Akinbami

Olofinsae

Ayeni

2018

PeerJ

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A serious concern is arising on the coexistence of extended-spectrum beta-lactamase (ESBL) and plasmid mediated quinolone resistance (PMQR) producing bacteria in animal husbandry, which could be transferred to humans, especially in strains that may not be routinely screened for resistance. This study therefore tested the prevalence of ESBL and PMQR genes in selected bacteria isolated from poultry faeces. Faecal droppings of birds were collected from 11 farms in five states in South Western Nigeria. Bacteria were isolated from the samples on cefotaxime supplemented plates and identified with MALDI-TOF. The MIC was determined using VITEK system and resistance genes were detected with PCR. A total of 350 strains were isolated from different samples and selected strains were identified as 23 Klebsiella pneumonia, 12 Morganella morganii, seven Leclercia adecarboxylata and one Citrobacter freundii. All the species were resistant to gentamycin, trimethoprim/sulphamethaxole, tobramycin, piperacillin, cefotaxime and aztreonam (except Morganella morganii strains which were mostly susceptible to aztreonam). All the tested strains were susceptible to imipenem, meropenem and amikacin. All Leclercia adecarboxylata strains were resistant to ceftazidime, cefepime and fosfomycin while all Morganella morganii strains were resistant to fosfomycin, moxifloxacin and ciprofloxacin. All tested species were generally sensitive to ciprofloxacin except Morganella morganii strains which were resistant to ciprofloxacin. The resistance to ciprofloxacin, ceftazidime, cefepime, tigercylin, colistin and fosfomycin were 65%, 40%, 23%,, 7%, 33%, 48% respectively while the prevalence of SHV, TEM and CTX genes were 42%, 63%, 35% respectively. 9.3% of the isolates had the three ESBL genes, 2.33% had qnrA gene, 4.65% had qnr B gene while none had qnrS gene. The most prevalent PMQR gene is Oqxb (25.58%) while 6.98% had the qep gene. Klebsiella pneumoniae generally had both ESBL and PMQR genes. The high prevalence of extended spectrum beta-lactamase genes in the studied strains calls for caution in the use of beta lactam antibiotics in poultry feeds. This is the first report of the occurrence of extended spectrum beta-lactamase and plasmid mediated quinolone resistance genes in Morganella morganii and Leclercia adecarboxylata strains isolated from poultry faeces.

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“…It is worth mentioning that ESBL producers co-resistant to non-beta lactam antibiotics is of special interest in this category; this is in view of the narrow choice of antibiotics that could be used in this category of patients (Al-Mayahie, 2013). Furthermore, in this country, CTX-M, SHV, TEM, and OXA ESBLs were described in clinical isolates of Morganella morganii with high resistance toward minocycline, trimethoprim- sulfamethoxazole and ciprofloxacin (Al-Muhanna et al, 2016). In parallel, VEB, PER, and OXA-10 were detected in high risk strains of P. aeruginosa : ST244, ST235, ST308, and ST654 (van Burgh et al, 2018).…”

Section: Distribution Of Multi-drug Resistant Organisms In Humansmentioning

confidence: 99%

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

et al. 2019

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In the last decade, extended-spectrum cephalosporin and carbapenem resistant Gram-negative bacilli (GNB) have been extensively reported in the literature as being disseminated in humans but also in animals and the environment. These resistant organisms often cause treatment challenges due to their wide spectrum of antibiotic resistance. With the emergence of colistin resistance in animals and its subsequent detection in humans, the situation has worsened. Several studies reported the transmission of resistant organisms from animals to humans. Studies from the middle east highlight the spread of resistant organisms in hospitals and to a lesser extent in livestock and the environment. In view of the recent socio-economical conflicts that these countries are facing in addition to the constant population mobilization; we attempt in this review to highlight the gaps of the prevalence of resistance, antibiotic consumption reports, infection control measures and other risk factors contributing in particular to the spread of resistance in these countries. In hospitals, carbapenemases producers appear to be dominant. In contrast, extended spectrum beta lactamases (ESBL) and colistin resistance are becoming a serious problem in animals. This is mainly due to the continuous use of colistin in veterinary medicine even though it is now abandoned in the human sphere. In the environment, despite the small number of reports, ESBL and carbapenemases producers were both detected. This highlights the importance of the latter as a bridge between humans and animals in the transmission chain. In this review, we note that in the majority of the Middle Eastern area, little is known about the level of antibiotic consumption especially in the community and animal farms. Furthermore, some countries are currently facing issues with immigrants, poverty and poor living conditions which has been imposed by the civil war crisis. This all greatly facilitates the dissemination of resistance in all environments. In the one health concept, this work re-emphasizes the need to have global intervention measures to avoid dissemination of antibiotic resistance in humans, animals and the environment in Middle Eastern countries.

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Molecular investigation of extended-spectrum beta-lactamase genes and potential drug resistance in clinical isolates ofMorganella morganii

Cited by 15 publications

References 30 publications

Molecular Exploring of Plasmid-mediated Ampc beta Lactamase Gene in Clinical Isolates of Proteus mirabilis

Molecular Exploring of Plasmid-mediated Ampc beta Lactamase Gene in Clinical Isolates of Proteus mirabilis

Prevalence of extended spectrum beta lactamase and plasmid mediated quinolone resistant genes in strains of Klebsiella pneumonia, Morganella morganii, Leclercia adecarboxylata and Citrobacter freundii isolated from poultry in South Western Nigeria

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

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