Molecular investigation to RNA and protein based interaction inducedin vivobiocompatibility of phytofabricated AuNP with embryonic zebrafish

Abstract

Implication of gold nanoparticles in industrial and day-today life products at extensive scale has raised concern about their toxicity to environment and human health. Moreover, quest of new technologies for production of biocompatible nanoparticles increased. This study explores the molecular toxicology of AuNP with enlightenment of their green synthesis using medicinal plant extract as reducing and stabilizing agent. Synthesized CAuNP were characterized for their physiochemical properties by standard techniques like FESEM, TEM, DLS, UV-Vis spectroscopy and FTIR. GCMS analysis revealed the involvement of-OH compounds for CAuNP synthesis. Determined size and zeta potential of CAuNP was found to be 21 ± 08 nm and-24 ± 11 mV with SPR peak at 554 nm. LC50 of CAuNP with zebrafish embryos was 69 ± 12 mg/ml compared to 52 ± 06 mg/ml of AuNP. Gold nanoparticles were found to exhibit concentration dependent morphological abnormalities with acute effect at cellular and molecular level. Experimental and computational analysis depicted the nanotoxicity of gold nanoparticles as a consequence of oxidative stress generation leading to apoptosis due to their influential interaction with Sod1, He1a and tp53 mRNA and proteins. The investigation deciphered the nanotoxicity of gold nanoparticles and suggested the implication of new green methodology for their future productions.

Cellular Investigations on Mechanistic Biocompatibility of Green Synthesized Calcium Oxide Nanoparticles with Danio rerio

“…CaONP uptake in adult zebrafish was evaluated by measuring the change in granularity through side scatter measurement by flow cytometry [24]. The reactive oxygen species (ROS) intensity in zebrafish exposed to different CaONP was measured for oxidative stress analysis.…”

Opportunistic gill infection is associated with TiO2 nanoparticle-induced mortality in zebrafish

“…As many fundamental cellular pathways involved in the response to toxicants or stresses are highly conserved between the zebrafish and mammals, it has been considered as a 'gold standard' for environmental toxicity assessment [18]. More recently, it has been demonstrated to be a useful model for evaluating the environmental health and safety impacts of engineered nanomaterials and nanoscale products, which are increasingly being produced as a result of developments in nanotechnology [17][18][19][20][21][22][23][24][25][26]. In addition, benefited by the size and transparent body, zebrafish could be used to observe the impact of NPs on the induction of reactive oxygen species and apoptosis pathways at the cellular level [17,20,23,24,26,27].…”

“…The direct effects of NP-induced toxicity have been revealed by recent studies [19][20][21][22][23][24][25][26][27][30][31][32][33][34]. However, the NP-induced collateral damages, such as interactions between injured fish and surrounding microorganisms, have not been considered, and their role remains unclear.…”

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

“…On the other hand, AuNPs as a functional tool acts as a spacer preventing graphene sheet aggregation, which would adversely affect cellular viability and colloidal stability attributes [54]. The biocompatible nature of core gold also adds to the selectivity profile of the whole system [55, 56]. Curcumin outstandingly serves dual roles in the synthesized CAG nanocomposite as an anti-cancer drug and a potent green reducing agent for simultaneous gold salt and GO reduction and composite fabrication.…”

“…Aiming for biological, anti-cancer applications require understanding of nanocomposite’s bio-physical characteristics [83]. Dynamic light scattering enables measuring particles’ hydrodynamic size distribution in biological medium, which is vital to assess dispersion stability [56, 83]. Fig 8 displays the average hydrodynamic diameter of CAG in RPMI-1640 medium (171.3 ± 30.3 nm).…”