Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum)

Nachit, M. M.; Elouafi, Ismahane; Pagnotta, M.A.; Saleh, Amiri; Iacono, E.; Labhilili, Mustapha; Asbati, A.; Azrak, Mohamad; Hazzam, Hani; Benscher, D.; Khairallah, M.; Ribaut, Jean-Marcel; Tanzarella, O. A.; Porceddu, E.; Sorrells, Mark E.

doi:10.1007/s001220051633

Cited by 104 publications

(78 citation statements)

References 32 publications

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“…More recent mapping studies in tetraploid (Nachit et al, 2001) and hexaploid wheat (Li et al, 1999) confirmed the isoenzyme studies and showed that LOX genes are located in colinear regions in wheat and barley suggesting that they are orthologous. These mapping results indicate that the wheat Lpx-2 locus corresponds to the barley LoxC gene (homoeologous group 5) whereas the wheat Lpx-1 locus corresponds to barley LoxA or LoxB locus (homoeologous group 4).…”

Section: Introductionmentioning

confidence: 70%

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A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat (Triticum turgidum ssp. durum)

Carrera

Echenique

Zhang

et al. 2007

Journal of Cereal Science

115

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The concentration of yellow carotenoid pigments in durum wheat grain is an important quality criterion and is determined both by their accumulation and by their degradation by lipoxygenase enzymes (Lpx loci). The existence of a duplication at the Lpx-B1 locus and the allelic variation for a deletion of the Lpx-B1.1 copy is reported. This deletion was associated with a 4.5-fold reduction in lipoxygenase activity and improved pasta color (Po0.0001) but not semolina color, suggesting reduced pigment degradation during pasta processing. A molecular marker for the deletion was mapped on chromosome 4B in a population obtained from the cross between durum line UC1113 and variety Kofa. A second lipoxygenase locus, designated Lpx-A3, was mapped on the homoeologous region on chromosome 4A and was associated with semolina and pasta color (Po0.01) but not with lipoxygenase activity in the mature grain. Selection for both the UC1113 allele for Lpx-A3 and the Kofa Lpx-B1.1 deletion resulted in a 10% increase in yellow scores for dry pasta relative to the opposite allele combination. This result indicates that the markers and the new allelic variants reported here will be useful tools to manipulate the wheat Lpx loci and to improve pasta color. r

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Section: Introductionmentioning

confidence: 70%

“…Activity is expressed as nanomoles of hydroperoxides per gram of semolina per min using absorbance at 234 nm and a 1-cm light path cell Nachit et al, 2001;van Mechelen et al, 1999). The Lpx-B1 locus was mapped on the short arm of chromosome 4B, whereas the Lpx-A3 locus was mapped on the long arm of chromosome 4A.…”

Section: Evolution Of Lox Genes In the Triticeaementioning

confidence: 99%

A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat (Triticum turgidum ssp. durum)

Carrera

Echenique

Zhang

et al. 2007

Journal of Cereal Science

115

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“…According to Roder et al (1998) and Harker et al (2001), Xgwm312 and Xwmc170, the two microsatellites most closely linked to the locus, map to the long arm of chromosome 2A in hexaploid wheat. Nachit et al (2001) found markers that map to chromosome 2AL in hexaploid wheat map to a similar interval in tetraploid wheat. The Nax1 locus identified in this study maps to an approximate position between 6.0 and 14.0 cM from the centromere on chromosome 2AL.…”

Section: Discussionmentioning

confidence: 90%

A locus for sodium exclusion (Nax1), a trait for salt tolerance, mapped in durum wheat

Lindsay

Lagudah

Hare

et al. 2004

Functional Plant Biol.

210

123

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Abstract. Salinity affects durum wheat [Triticum turgidum L. ssp. durum (Desf.)] more than it affects bread wheat (Triticum aestivum L.), and results in lower yield for durum wheat cultivars grown on salt-affected soils. A novel source of salt tolerance in the form of a sodium exclusion trait, identified previously in a screen of tetraploid wheat germplasm, was mapped using a QTL approach. The trait, measured as low Na + concentration in the leaf blade, was mapped on a population derived from a cross between the low Na + landrace and the cultivar Tamaroi. The use of AFLP, RFLP and microsatellite markers identified a locus, named Nax1 (Na exclusion), on chromosome 2AL, which accounted for approximately 38% of the phenotypic variation in the mapping population. Markers linked to the Nax1 locus also associated closely with low Na + progeny in a genetically unrelated population. A microsatellite marker closely linked to the Nax1 locus was validated in genetically diverse backgrounds, and proven to be useful for marker-assisted selection in a durum wheat breeding program.

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“…The Langdon BAC library as a tool to characterize polyploid evolution Durum wheat is a relatively young polyploid with extensive colinearity between the two genomes (Blanco et al 1998;Nachit et al 2001). This library will provide a valuable tool to characterize the genomic changes that have occurred during these early stages of polyploidization.…”

Section: Discussionmentioning

confidence: 99%

Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

et al. 2003

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Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, genomes AB) is an economically important cereal used as the raw material to make pasta and semolina. In this paper we present the construction and characterization of a bacterial artificial chromosome (BAC) library of tetraploid durum wheat cv. Langdon. This variety was selected because of the availability of substitution lines that facilitate the assignment of BACs to the A and B genome. The selected Langdon line has a 30-cM segment of chromosome 6BS from T. turgidum ssp. dicoccoides carrying a gene for high grain protein content, the target of a positional cloning effort in our laboratory. A total of 516,096 clones were organized in 1,344 384-well plates and blotted on 28 high-density filters. Ninety-eight percent of these clones had wheat DNA inserts (0.3% chloroplast DNA, 1.4% empty clones and 0.3% empty wells). The average insert size of 500 randomly selected BAC clones was 131 kb, resulting in a coverage of 5.1-fold genome equivalents for each of the two genomes, and a 99.4% probability of recovering any gene from each of the two genomes of durum wheat. Six known copy-number probes were used to validate this theoretical coverage and gave an estimated coverage of 5.8-fold genome equivalents. Screening of the library with 11 probes related to grain storage proteins and starch biosynthesis showed that the library contains several clones for each of these genes, confirming the value of the library in characterizing the organization of these important gene families. In addition, characterization of fingerprints from colinear BACs from the A and B genomes showed a large differentiation between the A and B genomes. This library will be a useful tool for evolutionary studies in one of the best characterized polyploid systems and a source of valuable genes for wheat. Clones and high-density filters can be requested at

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Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum)

Cited by 104 publications

References 32 publications

A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat (Triticum turgidum ssp. durum)

A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat (Triticum turgidum ssp. durum)

A locus for sodium exclusion (Nax1), a trait for salt tolerance, mapped in durum wheat

Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

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