Molecular magnetic resonance probe targeting VEGF165: preparation and <i>in vitro</i> and <i>in vivo</i> evaluation

You, Xiaoguang; Tu, Rong; Peng, Mingli; Bai, Yujie; Tan, Mingqian; Li, Han-Jian; Guan, Jing; Wen, Liang

doi:10.1002/cmmi.1584

Cited by 24 publications

(17 citation statements)

References 23 publications

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“…18 Recently, an aptamer coupled with USPIO was fabricated to target vascular endothelial growth factor 165 overexpressed by some tumors. 19 However, till now, reports on the application of aptamer-based MR probes specific for HCC are lacking. In this study, we successfully developed a novel HCC-targeted MR probe by conjugating GPC3-specific aptamer with USPIO.…”

Section: Discussionmentioning

confidence: 99%

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A GPC3-specific aptamer-mediated magnetic resonance probe for hepatocellular carcinoma

Zhao

Liu

Dong

et al. 2018

IJN

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PurposeTo construct and test a hepatocellular carcinoma (HCC)-targeted magnetic resonance probe based on a glypican-3 (GPC3)-specific aptamer (AP613-1) with ultrasmall superpara-magnetic iron oxide (USPIO).MethodsOleic acid-coated USPIO nanoparticles were modified with amino polyethylene glycol on the surface. Amino groups of the USPIO nanoparticles were reacted with the carboxyl group of 5′ carboxyl-modified AP613-1, forming an aptamer-mediated USPIO (Apt-USPIO) probe. The material characterization of this probe including transmission electron microscopy (TEM), zeta potential, dynamic laser scattering, and magnetic behavior was carried out. The targeting efficiency and magnetic resonance imaging (MRI) performance of Apt-USPIO were evaluated both in vitro and in vivo with USPIO alone as a control. The cytotoxicity and bio-compatibility of Apt-USPIO and USPIO were analyzed by cell counting kit-8 tests in vitro and animal experiments in vivo.ResultsTEM imaging revealed that the Apt-USPIO nanoparticles were spherical in shape and well dispersed. Specific uptake of Apt-USPIO in Huh-7 cells could be observed using the Prussian blue staining test; however, no uptake of USPIO could be found. In vitro phantom T2-weighted MRI showed a significant decrease of the signal intensity in Apt-USPIO-incubated Huh-7 cells compared to USPIO-incubated Huh-7 cells. In vivo T2-weighted MRI showed significantly negative enhancement in the Huh-7 tumors enhanced with Apt-USPIO, whereas no enhancement was found with USPIO alone. Excellent biocompatibility of Apt-USPIO and USPIO was also demonstrated.ConclusionIn this study, a molecular MRI probe which was highly specific to GPC3 on HCC was successfully prepared. Our results validated the targeted imaging effect of this Apt-USPIO probe in vivo for GPC3-expressing HCCs in xenograft mice.

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Section: Discussionmentioning

confidence: 99%

“…17 Recently, studies have shown the efficacy of aptamer-iron oxide probes for in vivo tumor-targeted MRI. 18,19 However, to the best of our knowledge, an aptamer-based MR probe specific for HCC has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

A GPC3-specific aptamer-mediated magnetic resonance probe for hepatocellular carcinoma

Zhao

Liu

Dong

et al. 2018

IJN

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“…These iron/protein nanocontainers inherently home to cancer cells that overexpress the transferrin receptor 1 (TfR1). Finally, it should be noted that DNA and RNA aptamers have been widely used to engineer MNPs for specific molecular recognition …”

Section: Surface Engineering Of Mnpsmentioning

confidence: 99%

Magnetic‐Nanoparticle‐Based Immunoassays‐on‐Chip: Materials Synthesis, Surface Functionalization, and Cancer Cell Screening

Zhu

Kekalo

Ndong

et al. 2016

Adv Funct Materials

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The unique properties of magnetic nanoparticles (MNPs), coupled with versatile surface engineering techniques, have led to a rising class of screening methods that enable separation of specific cell populations from complex biological samples. The growing sophistication and efficiency of these methods have far reaching implications for both fundamental research and clinical applications. In this study, the synthesis and surface engineering of MNPs is reviewed. Here, a model is introduced to illustrate how MNP morphology and particle–particle interactions influence magnetization, which is a key consideration in designing and selecting MNPs for efficient cell separations. Building upon these themes, immunomagnetic assays for capturing, isolating, and characterizing rare cell types from complex biological mixtures are reviewed. Although the focus of this study is on circulating tumor cells, these same techniques can be applied in screening for other rare cells of interest, such as various stem cell populations. In conclusion, current challenges and future directions for magnetic ‐nanomaterial‐based cell screening systems are discussed.

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“…Thus, an aptamer targeting VEGF165 was chemically linked with ultrasmall super paramagnetic iron oxide (USPIO) nanoparticles. This VEGF165-aptamer-USPIO enhanced the contrast of T2-weighted imaging in the liver cancer of mice xenografts (Figure 2) [117]. …”

Section: Aptamer-based Diagnosismentioning

confidence: 99%

Section: Aptamer-based Diagnosismentioning

confidence: 99%

Aptamers and Their Significant Role in Cancer Therapy and Diagnosis

Prakash

Rajamanickam

2015

Biomedicines

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Aptamers are nucleic acid/peptide molecules that can be generated by a sophisticated, well-established technique known as Systematic Evolution of Ligands by EXponential enrichment (SELEX). Aptamers can interact with their targets through structural recognition, as in antibodies, though with higher specificity. With this added advantage, they can be made useful for clinical applications such as targeted therapy and diagnosis. In this review, we have discussed the steps involved in SELEX process and modifications executed to attain high affinity nucleic acid aptamers. Moreover, our review also highlights the therapeutic applications of aptamer functionalized nanoparticles and nucleic acids as chemo-therapeutic agents. In addition, we have described the development of “aptasensor” in clinical diagnostic application for detecting cancer cells and the use of aptamers in different routine imaging techniques, such as Positron Emission Tomography/Computed Tomography, Ultrasound, and Magnetic Resonance Imaging.

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Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation

Cited by 24 publications

References 23 publications

A GPC3-specific aptamer-mediated magnetic resonance probe for hepatocellular carcinoma

A GPC3-specific aptamer-mediated magnetic resonance probe for hepatocellular carcinoma

Magnetic‐Nanoparticle‐Based Immunoassays‐on‐Chip: Materials Synthesis, Surface Functionalization, and Cancer Cell Screening

Aptamers and Their Significant Role in Cancer Therapy and Diagnosis

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