Molecular Mapping of the Rcs3 Gene for Resistance to Frogeye Leaf Spot in Soybean

Abstract

1990). Greater than 60% yield loss due to FLS has been reported in the tropical environment of Nigeria Frogeye leaf spot (FLS) (caused by Cercospora sojina Hara) is a (Dashiell and Akem, 1991). foliar disease of soybean [Glycine max (L.) Merr.] that causes significant yield loss in southeastern USA. The Rcs 3 gene in soybean has At least five races of the fungus have been reported been reported to condition resistance to all known races of C. sojina. in the USA (Phillips and Boerma, 1981). Twenty-two Molecular mapping of the Rcs3 gene will be helpful in breeding races of C. sojina were identified in Brazil (Yorinori, soybean for resistance to FLS. The objective of this study was to map 1992). In a recent study designed to further elucidate the Rcs 3 gene with DNA markers. The parents, 123 F 2 plants, and the extent of pathogen variability and to define a set of the F 2:3 families from the cross of 'Blackhawk' (susceptible) ϫ 'Davis' differential cultivars to aid in race identification of C. (resistant and original source of the Rcs3 gene) were scored for FLS sojina, D.V. Phillips identified over 40 races of the funreaction in the greenhouse after inoculation with race 5 of C. sojina. gus on a set of 38 differential cultivars (unpublished In addition, 'Wright' and the Wright 6 -Rcs 3 (near isoline of Wright) data, 1998). Three single genes conditioning resistance were also scored for FLS by the same protocol. A resistant and a to C. sojina are currently recognized by the Soybean susceptible DNA bulk were created by pooling the DNA of 15 resistant and 15 susceptible F 2 plants, respectively, from the Blackhawk ϫ

Diversidade genética da soja entre períodos e entre programas de melhoramento no Brasil

“…Kashi & Soller (1998), por exemplo, sugeriram que alguns microssatélites estejam associados à codificação de proteínas e também ao processo de transcrição em muitos organismos. Vários locos microssatélites em soja apresentaram associação com resistência a doenças em estudos de mapeamento, como os locos Satt244 e Satt547, que estão ligados à resistên-cia ao fungo Cercospora sojina Hara (Mian et al, 1999), os locos Satt082, Sat_001, Satt574 e Satt301, associados à resistência ao nematóide-de-cisto Heterodera glycines (Schuster et al, 2001) e o loco Satt309 (utilizado neste estudo) que está associado à resistência ao Fusarium solani f. sp. glycines (Fronza, 2003).…”

Identificação e validação de marcadores microssatélites ligados ao gene Rpp5 de resistência à ferrugem-asiática-da-soja

“…Estratégia similar também foi utilizada para mapear outros locos de resistência na soja (Mian et al, 1999;Gordon et al, 2006;Silva et al, 2008). Assim, foram testados 50 marcadores microssatélites, para verificar o polimorfismo entre os genitores, dos quais nove se apresentaram polimórficos e foram utilizados na discriminação dos "bulks" contrastantes.…”

GWAS of a soybean breeding collection from South East and South Kazakhstan for resistance to fungal diseases

“…Several soybean mapping populations were developed for genetic localization of QTL and genes associated with the soybean diseases, such as rhizoctonia root rot (RRR, caused by Rhizoctonia solani) (Zhao et al, 2005), fusarium root rot (FUS, caused by Fusarium spp.) (Stacey, 2008), phytophthora root rot (PRR, caused by Phytophthora sojae) (Zhang et al, 2013), frogeye leaf spot (FLS, caused by C. sojina) (Mian et al, 1999) and sclerotinia stem rot (SCL, caused by Sclerotinia sclerotiorum) (Zhao et al, 2015). The majority of these studies were based on use of SSR (simple sequence repeat) microsatellite markers.…”