Molecular marker-assisted sex control in half-smooth tongue sole (Cynoglossus semilaevis)

Growth differences and dimorphic expression of growth hormone (GH) in female and male Cynoglossus semilaevis after male sexual maturation

“…The low growth rate of males weakens the quality of the fish, and thus leads to an overall reduction in production. Recently, to solve the problem, artificial gynogenesis (Chen et al, 2008b) and molecular marker-assisted sex control techniques (Chen et al, 2008a) were developed in order to generate all-female stocks in C. semilaevis. However, C. semilaevis has a ZZ/ZW sex determination mechanisms and it is very difficult to obtain WW superfemales or distinguish WW superfemales from the progeny of ZW female × ZW sex-reversed male crosses.…”

“…The paraffin containing the tissues was sectioned in 6 μm, and stained with hematoxylin and eosin (Chen et al, 2008a). In total three sections of the testes or ovaries were evaluated from each fish by microscopical observation.…”

“…Genetic sex identification of C. semilaevis was determined according to Chen et al (2007;2008a). The genomic DNA from each fry was extracted using a traditional proteinase-K digestion and phenolchloroform protocol with RNase treatment (Blin and Stafford, 1976).…”

“…Cynoglossus semilaevis, is an important cultured marine fish species in China (Chen et al, 2008a) and showed significantly sexual dimorphism in growth. However, the physiologic mechanisms of sexual growth dimorphism in C. semilaevis remain unknown.…”

Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

“…Molecular marker technique has been proved to be an effective tool for the identification of genetic sex, the analysis of genetic diversity and sex control (Liu and Cordes 2004;Chen et al 2008). However, few reports on sex-specific molecular markers are available in teleosts ).…”