Molecular marker heterozygosity and hybrid performance in indica and japonica rice

Zhang, Q.; Zhou, Z. Q.; Yang, Guliang; Xu, Conghao; Liu, K. D.; Maroof, M. A. Saghai

doi:10.1007/s001220050359

Cited by 31 publications

(32 citation statements)

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“…In order to find a better way to analyse the heterosis, an alternative model (Model 3) was proposed. The general consideration of the new model is that some markers have a favorable effect on the heterosis and some have a X 5 and X 6 in Model 1 and Model 2 mean general heterozygosity and special heterozygosity respectively (Zhang et al , 1995(Zhang et al , 1996. Model 3 was interpreted in the Materials and methods section b Corresponding parameters and their statistical significance, which were calculated by SAS software package Version 6.12, were estimated on the basis of the dry weight of ten F 1 plants c Data of one year were applied to the equation to predict the plant biomass performance in the other year.…”

Section: Resultsmentioning

confidence: 99%

“…Heterosis is a phenomenon with a very complex genetic basis and is controlled by a number of genes (Zhang et al 1996;Yu et al 1997). This set of positive markers identified in this experiment is probably just a part of the total.…”

Section: The Significance Of Super Active Markers (Sams) In Breedingmentioning

confidence: 99%

“…Zhang et al (1994) posed the idea of specific heterozygosity to assess the level of correlation of heterozygosity with heterosis and found a good correlation between specific heterozygosity and the grain yield heterosis of rice. However, later on, further studies showed that the extent of correlation also varied widely from one trait to another and from one dataset to another (Zhang et al 1995(Zhang et al , 1996.…”

Section: Introductionmentioning

confidence: 99%

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Association of RFLP markers and biomass heterosis in trigenomic hybrids of oilseed rape (Brassica napus × B. campestris)

2002

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Laboratory screening with DNA-based markers and field measurements of biomass production were carried out on each of the 120 trigenomic hybrids, obtained by interspecific hybridization between Brassica napus (AACC) and Brassica campestris (A'A'). The objective of this study was to elucidate the relationship between molecular markers and biomass heterosis of the interspecific hybrid between B. napus and B. campestris, which has been explored practically in rapeseed production for many years. The experiment was first carried out on 65 trigenomic hybrids in 1999. The average over-mid-parent heterosis of biomass production was around 30%, and the highest value was 175.4%. In the following year, the observation was expanded to 120 trigenomic hybrids and the best average over-mid-parent heterosis was 93%. A total of 1,477 DNA fragments, generated by Southern hybridization with 50 Brassica cDNA clones and 25 Arabidopsis EST clones, was scored across their parental lines. One hundred and twenty six and 215 fragments were identified as significantly associated with biomass production respectively in the 2 successive years. Using these active markers, a statistical model to resolve the heterosis is proposed and a new way to make use of the subgenomic heterosis is also discussed.

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Section: Resultsmentioning

confidence: 99%

Section: The Significance Of Super Active Markers (Sams) In Breedingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Association of RFLP markers and biomass heterosis in trigenomic hybrids of oilseed rape (Brassica napus × B. campestris)

2002

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“…There have been a number of studies in rice addressing the genetic basis of heterosis and the relationship of heterosis to molecular heterozygosity and gene expression (Zhang et al 1994(Zhang et al , 1995(Zhang et al , 1996Xiao et al 1995;Yu et al 1997;Xiong et al 1998). The present study has identi®ed three classes of patterns of cytosine methylation characterized by dierences in degree of methylation between the hybrid and the parent al lines: (1) the same level of methylation in the parents and hybrid; (2) an increased level of methylation in the hybrid compared to the parents, and (3) a decreased level of methylation in the hybrid.…”

Section: Discussionmentioning

confidence: 96%

Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique

Xiong¹,

Xu²,

et al. 1999

Self Cite

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DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes. In this study, we assessed the extent and pattern of cytosine methylation in the rice genome, using the technique of methylation-sensitive amplified polymorphism (MSAP), which is a modification of the amplified fragment length polymorphism (AFLP) method that makes use of the differential sensitivity of a pair of isoschizomers to cytosine methylation. The tissues assayed included seedlings and flag leaves of an elite rice hybrid, Shanyou 63, and the parental lines Zhenshan 97 and Minghui 63. In all, 1076 fragments, each representing a recognition site cleaved by either or both of the isoschizomers, were amplified using 16 pairs of selective primers. A total of 195 sites were found to be methylated at cytosines in one or both parents, and the two parents showed approximately the same overall degree of methylation (16.3%), as revealed by the incidence of differential digestion by the isoschizomers. Four classes of patterns were identified in a comparative assay of cytosine methylation in the parents and hybrid; increased methylation was detected in the hybrid compared to the parents at some of the recognition sites, while decreased methylation in the hybrid was detected at other sites. A small proportion of the sites was found to be differentially methylated in seedlings and flag leaves; DNA from young seedlings was methylated to a greater extent than that from flag leaves. Almost all of the methylation patterns detected by MSAP could be confirmed by Southern analysis using the isolated amplified fragments as probes. The results clearly demonstrate that the MSAP technique is highly efficient for large-scale detection of cytosine methylation in the rice genome. We believe that the technique can be adapted for use in other plant species.

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“…With the advent of molecular markers and construction of linkage maps, attempts were made by different scientific groups to characterize the genetic basis of heterosis (Xiao et al 1995;Yu et al 1997;Li et al 2001;Luo et al 2001;Zhang et al 2001;Xing et al 2002;Hua et al , 2003Frascaroli et al 2007) and to understand the relationship of heterosis with molecular heterozygosity in rice (Zhang et al 1994(Zhang et al , 1995(Zhang et al , 1996Saghai Maroof et al 1997;Liu et al 1998;Zhao et al 1999). However, these studies have provided no information about the genes and/or the molecular mechanisms involved in the manifestation of heterosis.…”

Section: Introductionmentioning

confidence: 99%

Alterations in inheritance pattern and level of cytosine DNA methylation, and their relationship with heterosis in rice

Sakthivel

Girishkumar²,

Ramkumar

et al. 2010

Euphytica

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Novel hybrid-specific/heterotic gene expression patterns observed from expression studies suggest the need to characterize the underlying regulatory mechanism(s) to reveal the biological basis of heterosis in crop plants. To gain an insight into the molecular basis of heterosis in rice, we investigated the inheritance pattern and level of cytosine methylation, a major epigenetic regulatory mechanism, in the leaf tissue of an elite Indian rice hybrid KRH2 and its parents at three stages (15 day-old, 35 day-old seedling and flag leaf) and their correlation with heterosis using methylation-sensitive amplification polymorphism (MSAP) technique. In contrast to parents, the level of methylation was high during initial growth stages and reduced as the hybrid grew. Even though, a majority of cytosine methylation profiles were transmitted to hybrid at all stages, a considerable level (25.4%) of cytosine methylation pattern was observed to be novel in hybrid and some of these altered loci were identified to code for known/hypothetical proteins. It was also observed that demethylation events occurred more predominantly during early stages than hypermethylation while the frequency of demethylation events decreased in flag leaf with a remarkable increase in hypermethylation in the hybrid. These bi-directional events in methylation in KRH2 were validated in six hybrids with different levels of grain yield heterosis and the level of cytosine methylation in the hybrid was observed to be significantly correlated with heterosis.

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Molecular marker heterozygosity and hybrid performance in indica and japonica rice

Cited by 31 publications

References 0 publications

Association of RFLP markers and biomass heterosis in trigenomic hybrids of oilseed rape (Brassica napus × B. campestris)

Association of RFLP markers and biomass heterosis in trigenomic hybrids of oilseed rape (Brassica napus × B. campestris)

Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique

Alterations in inheritance pattern and level of cytosine DNA methylation, and their relationship with heterosis in rice

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