Molecular Markers in Bamboos: Understanding Reproductive Biology, Genetic Structure, Interspecies Diversity, and Clonal Fidelity for Conservation and Breeding

Konzen, Enéas Ricardo; Pozzobon, Luciano Cesar; Souza, Denys Matheus Santana Costa; Fernandes, Sérgio Bruno; Campos, Wellington Ferreira; Brondani, Gilvano Ebling; Carvalho, Dulcinéia de; Tsai, Siu Mui

doi:10.1007/978-981-16-1310-4_2

Cited by 2 publications

(6 citation statements)

References 125 publications

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“…RAPD-RFLP markers were efficient for molecular identification of bamboo genera and species, providing a cost-effective and accurate method for conservation, management and plant breeding (Konzen et al, 2017;Konzen et al, 2021b). The lack of genetic variations in the micropropagation process suggests that the protocol was efficient, with no interference from the use of antibiotics in the material's decontamination or the number of subcultures.…”

Section: Genetic Fidelitymentioning

confidence: 99%

“…Some clonal variations, through the production of calluses (e.g., indirect organogenesis), can occur due to prolonged in vitro culture time (Lin et al, 2019). For checking for the occurrence of these events, genetic fidelity tests and anatomical tissue analyses can be performed after micropropagated plants acclimatization (Konzen et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

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Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Teixeira

Gonçalves

Modesto

et al. 2021

CERNE

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Background:We have developed a micropropagation technique methodology to clonal microgarden formation of Bambusa vulgaris selected adult plant. Collection site (i.e., stock plant cultivated in different environments) and seasonality of shoot collection (i.e., months of the year) effects on in vitro culture were evaluated. Explants that showed normal development were ex vitro rooted in miniincubator system, and three culture media (WPM, MS and deionized water + agar) supplemented with plant growth regulators (IBA, NAA and BAP) and antibiotic (streptomycin sulphate and culture medium free -control) were evaluated. Micropropagated plants were acclimatized in a shadow house and transferred to a semi-hydroponic system for establishment of a clonal micro-garden. The efficacy of the cloning protocol was determined with genetic fidelity analysis by ISSR molecular markers.Results: Considering all inoculations, 21.9% of nodal segments were in vitro established in nine shoot collections. The rooting percentage was 36.6%, and no interactions were observed between the use of culture medium and antibiotic. Culture medium free antibiotic resulted in 80.0% of survival and 50.0% of adventitious rooting. Micropropagated plants presented adequate growth and adaptation to ex vitro conditions in the clonal micro-garden. Molecular analyses by ISSR markers indicated the absence of genetic variations, and histological analyses revealed normal adventitious root formation originating from meristematic cells. Conclusion:The formation of a clonal micro-garden was demonstrated, proving the feasibility of the tested technique. Our results contribute to the development of a clonal propagation protocol for B. vulgaris selected adult plants.

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Section: Genetic Fidelitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Teixeira

Gonçalves

Modesto

et al. 2021

CERNE

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…Micropropagation is important technique for cloning and improving selected phenotypes (BHADRAWALE et al, 2018;TAMBARUSSI et al, 2017;SILVEIRA et al, 2020;KONZEN et al, 2021a), as it can generate plants with the same genetic feature of the parent plant (i.e., selected plant in adult stage) on industrial scale (HARTMANN et al, 2011;BRONDANI et al, 2017;KONZEN et al, 2021b). However, in some cases, this technology can induce genetic and morphological changes, known as somaclonal variations (LARKIN; SCOWCROFT, 1981;KONZEN et al, 2021b). This variation is considered a disadvantage when the aim is cloning, and for this reason, the development of further studies on genetic stability through in vitro cultivation is important for understanding the factors involved in the clonal production of bamboo species (KONZEN et al, 2017;RAMAKRISHNAN et al, 2020;KONZEN et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

“…However, in some cases, this technology can induce genetic and morphological changes, known as somaclonal variations (LARKIN; SCOWCROFT, 1981;KONZEN et al, 2021b). This variation is considered a disadvantage when the aim is cloning, and for this reason, the development of further studies on genetic stability through in vitro cultivation is important for understanding the factors involved in the clonal production of bamboo species (KONZEN et al, 2017;RAMAKRISHNAN et al, 2020;KONZEN et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

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CLONAL MICROPLANT PRODUCTION, MORPHOLOGICAL EVALUATION AND GENETIC STABILITY OF Dendrocalamus asper (Schult. & Schult.) Backer ex. K. Heyneke

Gonçalves

Souza

Molinari

et al. 2023

NAT

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Bamboo species have many commercial applications, considering that homogeneous plantations (formed from clonal plants) are essential to high sustainable biomass production. The cloning of selected plants on an industrial scale through in vitro cultivation has many advantages, being important for the supply of plants in sufficient quantity and quality to meet commercial demand. The control of the cloning is the basis for an industrial scale, and its knowledge can optimize the process. This work aimed to evaluate the cloning of Dendrocalamus asper selected plant through micropropagation. Morphological features by scanning electron microscopy and genetic stability with ISSR molecular markers were evaluated. Four times of immersion in sodium hypochlorite (NaOCl) on in vitro establishment of nodal segments were evaluated. The established explants were transferred to a culture medium that was supplemented with three concentrations of 6-benzylaminopurine (BAP). Three concentrations of indole-3-butyric acid (IBA) to the in vitro adventitious rooting were evaluated. NaOCl application for 10 min resulted in 71.4 % of establishment in 30 d. Supplementation of the culture medium with 2.0 and 3.0 mg L-1 BAP de resulted in the highest averages for multiplication and elongation stages. The formation of adventitious roots occurred with 4.0 mg L-1 IBA of supplementation. Micropropagated plants showed normal morphological features and genetic stability, confirming the cloning of selected plant. Keywords: bamboo; micropropagation; vegetative propagation; In vitro culture; ISSR; plant growth regulator. Produção de microplantas clonais, avaliação morfofisiológica e estabilidade genética de Dendrocalamus asper (Schult. & Schult.) Backer ex. K. Heyneke RESUMO: Espécies de bambus apresentam diversas aplicações comerciais, visto que os plantios homogêneos (formados a partir de plantas clonais) são essenciais para a alta produção de biomassa sustentável. A clonagem de plantas selecionadas em escala industrial por meio do cultivo in vitro apresenta muitas vantagens, sendo uma importante ferramenta para o fornecimento de plantas em quantidade e qualidade suficientes para atender a demanda comercial. O controle da clonagem é a base para escala industrial, e seu conhecimento pode otimizar os processos. O trabalho teve como objetivo avaliar a clonagem de planta selecionada de Dendrocalamus asper por meio da técnica de micropropagação. Foram avaliadas as características morfológicas por microscopia eletrônica de varredura e estabilidade genética por meio de marcadores moleculares ISSR. Além disso, foram avaliados quatro tempos de imersão em hipoclorito de sódio (NaOCl) no estabelecimento in vitro de segmentos nodais. Os explantes estabelecidos foram transferidos para um meio de cultura que foi suplementado com três concentrações de benzilaminopurina (BAP). Por fim, foram avaliadas três concentrações de ácido indolbutírico (AIB) durante o enraizamento adventício in vitro. A adição de NaOCl por 10 min resultou em 71,4 % de estabelecimento em 30 d. A suplementação do meio de cultura com 2,0 e 3,0 mg L-1 BAP resultou nas maiores médias para as fases de multiplicação e alongamento. A formação de raízes adventícias ocorreu com a suplementação de 4,0 mg L-1 de AIB. Plantas micropropagadas apresentaram características morfológicas normais e estabilidade genética, confirmando a clonagem da planta selecionada. Palavras-chave: bambu; micropropagação; propagação vegetativa; cultivo in vitro; ISSR; regulador de crescimento vegetal.

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Molecular Markers in Bamboos: Understanding Reproductive Biology, Genetic Structure, Interspecies Diversity, and Clonal Fidelity for Conservation and Breeding

Cited by 2 publications

References 125 publications

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

CLONAL MICROPLANT PRODUCTION, MORPHOLOGICAL EVALUATION AND GENETIC STABILITY OF Dendrocalamus asper (Schult. & Schult.) Backer ex. K. Heyneke

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