Molecular Mechanism of Anti-Prostate Cancer Activity of<i>Scutellaria Baicalensis</i>Extract

Ye, Fei; Jiang, Shaosong; Volshonok, Helen; Wu, Josephine W.; Zhang, David Y.

doi:10.1080/01635580701268352

Cited by 52 publications

(35 citation statements)

References 37 publications

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“…We have previously demonstrated that S. baicalensis can inhibit uncontrolled cell division in many cell lines, including squamous cell cancer cells, prostate cancer cells, colon cancer cells, breast cancer, as well as hepatocellular carcinoma cells (8,13,17). This study further confirmed that S. baicalensis can inhibit HepG2 cell growth in vitro with an IC 50 of 360 µg/ml (Fig.…”

Section: Discussionsupporting

confidence: 88%

“…The lysate was sonicated 3 times for 15 s each time and then centrifuged at 14,000 rpm for 30 min at 4 • C. The protein concentration was determined with the BCA Protein Assay kit (PIERCE, Rockford, IL). 300 µg of lysated protein was loaded in one well across the entire width of 10% SDS polyacrylamide and separated by electrophoresis (17). After electrophoresis, the proteins were transferred electrophoretically to a nitrocellulose membrane that was then blocked for 1 h with blocking buffer either including 5% milk or 3% BSA in 1 × TBST containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 0.1% Tween-20.…”

Section: Pathway Arraymentioning

confidence: 99%

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The Effect ofScutellaria Baicalensison the Signaling Network in Hepatocellular Carcinoma Cells

Che

McMillen

et al. 2009

Nutrition and Cancer

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Scutellaria baicalensis is an anti-inflammatory and antineoplastic Chinese herbal therapy. We have previously shown that S. baicalensis can inhibit hepatocellular carcinoma (HCC) cell growth in vitro. In this study, we sought to determine the effect of S. baicalensis on the cell signaling network using our newly developed Pathway Array technology, which screens cell signaling pathways involved in cell cycle regulation. The HCC cell line (HepG2) was treated with S. baicalensis extract in vitro. The effect on the cell cycle was analyzed by flow cytometry, and the expression of various signaling proteins was assayed with Pathway Array. Our results indicate that S. baicalensis exerts a strong growth inhibition of the HepG2 cells via G(2)/M phase arrest. The Pathway Array analysis of 56 proteins revealed a total of 14 differentially expressed proteins or phosphorylations after treatment. Of these, 9 showed a dose-dependent decrease (p53, ETS1, Cdc25B, p63, EGFR, ERK1/2, XIAP, HIF-2alpha, and Cdc25C) whereas one demonstrated a dose-dependent increase (Cyclin E) after treatment with 200 microg/ml of S. baicalensis. Using computer simulation software, we identified additional hubs in the signaling network activated by S. baicalensis. These results indicate that S. baicalensis exerts a broad effect on cell signaling networks leading to a collective inhibition of cell proliferation.

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Section: Discussionsupporting

confidence: 88%

Section: Pathway Arraymentioning

confidence: 99%

The Effect ofScutellaria Baicalensison the Signaling Network in Hepatocellular Carcinoma Cells

Che

McMillen

et al. 2009

Nutrition and Cancer

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“…The activation of Akt by PGE 2 was blocked by AG 1478 (an EGF receptor specific inhibitor), and LY 294002 and Akt inhibitor prevented PGE 2 -induced p44/42MAPK phosphorylation. In addition, PGE 2 -induced phosphorylation of p44/42 MAPK led to activation of CDKs in human prostate cancer cell lines[24]. Furthermore, in human thyroid tumor cells, p44/42 MAPK inhibitor prevented EGF-induced stimulation of cyclin D1, which is an important intermediate in inducing the cell cycle cascade…”

mentioning

confidence: 99%

Interaction between PGE2 and EGF receptor through MAPKs in mouse embryonic stem cell proliferation

Yun

Lee

Ryu

et al. 2009

Cell. Mol. Life Sci.

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Identifying the small molecules that permit precise regulation of embryonic stem (ES) cell proliferation should further support our understanding of the underlying molecular mechanisms of self renewal. In the present study, we showed that PGE(2) increased [(3)H]-thymidine incorporation in a time and dose dependent manner. In addition, PGE(2) increased the expression of cell cycle regulatory proteins, the percentage of cells in S phase and the total number of cells. PGE(2) obviously increased E-type prostaglandin (EP) receptor 1 mRNA expression level compare to 2, 3, 4 subtypes. EP1 antagonist also blocked PGE(2)-induced cell cycle regulatory protein expression and thymidine incorporation. PGE(2) caused phosphorylation of protein kinase C, Src, epidermal growth factor (EGF) receptor, phosphatidylinositol 3-kinase (PI3K)/Akt phosphorylation, and p44/42 mitogen-activated protein kinase (MAPK), which were blocked by each inhibitors. In conclusion, PGE(2)-stimulated proliferation is mediated by MAPK via EP1 receptor-dependent PKC and EGF receptor-dependent PI3K/Akt signaling pathways in mouse ES cells.

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“…As mentioned above, S. baicalensis is widely used in anti-inflammatory and anti-cancer therapy. Ye et al [55] reported more detailed pharmacological functions of these extracts in human prostate cancer cell lines. They showed that they can inhibit tumor growth with an IC 50 value of 0.2 ~ 0.4 mg/mL and activate prostate-specific antigen production.…”

Section: Anti-prostate Cancer Activitymentioning

confidence: 97%

“…More recently, the activity of S. baicalensis extract against prostate cancer has been reported [55]. As mentioned above, S. baicalensis is widely used in anti-inflammatory and anti-cancer therapy.…”

Section: Anti-prostate Cancer Activitymentioning

confidence: 98%

Potential agents for cancer and obesity treatment with herbal medicines from the green garden

Park

Kim

Park

et al. 2011

Biotechnol Bioproc E

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Many classes of bioactive drug-like molecules derived from traditional herbal plants are becoming attractive as alternative medicines for the treatment of severe chronic diseases such as cancer and obesity. A set of chemically synthesized drugs that is capable of both inhibiting cancer growth and reducing body weight for treatment of obesity have severe side effects including nausea, vomiting, diarrhea as well as producing increased blood pressure and headache, respectively. For decades, drug candidates from herbal plants have been considered as potential therapeutic agents because they are generally safer, less toxic, and have fewer lethal side effects than chemically synthesized or semi-synthetic drugs. Understanding the key factors affecting pharmacological effects and clinical outcomes has been a critical theme of natural product research. However, standardized sample preparation methods, well-controlled scientific studies, and validation studies are needed before herbal therapeutics can be introduced into the global market. This review will address the current advances in using traditional herbal plants, including the pharmacological effects and the challenges faced during the development of new drugs. The safety issues associated with toxicity and the effectiveness of the herbs in specific diseases such as cancer and obesity are also discussed.

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Molecular Mechanism of Anti-Prostate Cancer Activity ofScutellaria BaicalensisExtract

Cited by 52 publications

References 37 publications

The Effect ofScutellaria Baicalensison the Signaling Network in Hepatocellular Carcinoma Cells

The Effect ofScutellaria Baicalensison the Signaling Network in Hepatocellular Carcinoma Cells

Interaction between PGE2 and EGF receptor through MAPKs in mouse embryonic stem cell proliferation

Potential agents for cancer and obesity treatment with herbal medicines from the green garden

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