Molecular mechanisms of ampelopsin from Ampelopsis megalophylla induces apoptosis in HeLa cells

Abstract

Ampelopsin (AMP) is an active ingredient of flavonoid compounds that is extracted from Ampelopsis megalophylla Diels et Gilg. The present study aimed at investigating the antitumor activities of AMP and the possible underlying molecular mechanisms in HeLa cells. A total of three types of tumor cell were selected to screen antitumor activities for AMP using the MTT assay. Flow cytometry was used to analyze the cell apoptotic proportion and the cell cycle. Rhodamine 123 staining was used to determine changes in mitochondrial transmembrane potential. Western blot analysis was used to determine the expression of apoptosis-associated proteins. The results of the present study demonstrated that AMP may inhibit the viability of HeLa cells in a dose- and time-dependent manner. Changes in morphology were observed using fluorescence microscopy. In addition, Annexin V-fluorescein isothiocyanate/propidium iodide (PI) double staining revealed that AMP induced apoptosis in a concentration-dependent manner and PI staining indicated that HeLa cells were arrested in S phase. Furthermore, western blot analysis demonstrated that AMP treatment induced apoptosis through activation of caspases 9 and 3, which was validated by the increasing ratio of B-cell lymphoma 2 (Bcl-2)-associated X protein to Bcl-2. Additionally, the loss of mitochondrial transmembrane potential and the release of cytochrome c suggested that AMP-induced apoptosis was associated with the mitochondrial pathway. Taken together, these results indicate that AMP may induce apoptosis via the mitochondrial signaling pathway in HeLa cells.

Eriodictyol Inhibits Proliferation, Metastasis and Induces Apoptosis of Glioma Cells via PI3K/Akt/NF-κB Signaling Pathway

“…This suggests that eriodictyol might induce DNA replication damage in glioma cells (Bailon-Moscoso et al, 2017). Similar to our findings, other anti-cancer flavonoid compounds, such as ampelopsin, quercetin, and baicalein also arrest the cell cycle at the S phase (Mu et al, 2016;Srivastava et al, 2016;Cheng et al, 2017).…”

Dihydromyricetin Acts as a Potential Redox Balance Mediator in Cancer Chemoprevention

“…The suppressing effect of DHM on the MDA-MB-231 breast cancer cell line was reported through ROS generation, ER stress pathway, and inhibition of mTOR [28,31]. DHM treatment dose-dependently inhibited the growth of HeLa cells by inducing apoptosis through activation of caspases 9 and 3 and increasing the ratio of Bax protein to Bcl-2 [44]. In A549 human adenocarcinoma lung epithelial cells, DHM decreased XIAP and survivin expression levels and cleaved poly(ADP-ribose) polymerase.…”

“…DHM effectively showed anticancer activity in a variety of cancers such as breast cancer, hepatocellular carcinoma, melanoma, ovarian cancer, lung cancer, cervical carcinoma, glioma, and osteosarcoma [32,33,[42][43][44][45][46]. Among the treatments for different cancer cells, DHM has a broad dosage from 1 to 1000 μM with a duration from 6 to 72 h, presenting cell proliferation inhibition and apoptosis-inducing effects ( Table 1).…”

Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line

“…Its tender leaves and stems are commonly used as a herbal tea and to prevent hypertension, particularly in the west of Hubei province (14). Pharmacological research has suggested that A. megalophylla has hypertensive, antiinflammatory, antiviral, antitumor, hyperglycemic, antimicrobial, hepatoprotective, neuroprotective, and antioxidant activities (15)(16)(17). Notably, its principal effective components are flavonoids and total flavone extract (TFE), which contains compounds, such as ampelopsin, myricetin and myricitrin.…”