Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation

Yatskevich, Stanislau; Kroonen, Jessie S; Alfieri, Claudio; Tischer, Thomas; Howes, Anna C.; Clijsters, Linda; Yang, Jing; Zhang, Ziguo; Yan, Kaige; Vertegaal, Alfred C.O.; Barford, David

doi:10.1016/j.celrep.2021.108929

Cited by 22 publications

(20 citation statements)

References 50 publications

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“…One perspective is that APC/C-mediated ubiquitination drives the disassembly of MAD2 and BUBR1, and then UBCH10 mediates the inactivation of the SAC 52 . In addition, SUMOylation of the APC/C attenuates MCC-mediated suppression of APC/C CDC20 activity, which permits the catalytic activity of the APC/C before the SAC is turned off 72 . As a subunit of APC/C, APC15 catalyses the disassembly/degradation of APC/C-bound MCC 73 .…”

Section: Discussionmentioning

confidence: 99%

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I

et al. 2022

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Chromosome segregation is initiated by cohesin degradation, which is driven by anaphase-promoting complex/cyclosome (APC/C). Chromosome cohesin is removed by activated separase, with the degradation of securin and cyclinB1. Dynamin-related protein 1 (DRP1), a component of the mitochondrial fission machinery, is related to cyclin dynamics in mitosis progression. Here, we show that DRP1 is recruited to the kinetochore by centromeric Centromere protein F (CENP-F) after nuclear envelope breakdown in mouse oocytes. Loss of DRP1 during prometaphase leads to premature cohesin degradation and chromosome segregation. Importantly, acute DRP1 depletion activates separase by initiating cyclinB1 and securin degradation during the metaphase-to-anaphase transition. Finally, we demonstrate that DRP1 is bound to APC2 to restrain the E3 ligase activity of APC/C. In conclusion, DRP1 is a CENP-F-dependent atypical spindle assembly checkpoint (SAC) protein that modulates metaphase-to-anaphase transition by controlling APC/C activity during meiosis I in oocytes.

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Section: Discussionmentioning

confidence: 99%

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I

et al. 2022

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“…In addition, mass spectrometry analysis of APC/C showed a lactate‐dependent elevation of SUMO2/3 conjunctions. Previous studies have shown that SUMOylation of APC4 on residues K772 and K798 results in a substantial rearrangement of the WHB domain in APC2, facilitating the binding of UBE2C to APC/C for an efficient APC/C activation 3,4 . To further investigate the role of APC4 SUMOylation in lactate‐mediated UBE2C–APC/C interactions, Liu et al constructed APC4 K772/798R cells in which APC4 SUMOylation is abrogated.…”

Section: Figurementioning

confidence: 99%

“…Previous studies have shown that SUMOylation of APC4 on residues K772 and K798 results in a substantial rearrangement of the WHB domain in APC2, facilitating the binding of UBE2C to APC/C for an efficient APC/C activation. 3,4 To further investigate the role of APC4 SUMOylation in lactate-mediated UBE2C-APC/C interactions, Liu et al constructed APC4 K772/798R cells in which APC4 SUMOylation is abrogated. They found that the lactate-mediated interaction between UBE2C and APC/C was completely lost in these cells.…”

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confidence: 99%

Lactate: A critical regulator of cell proliferation via anaphase promoting complex remodeling

2023

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“…SUMOylation has been known to affect mitosis in various ways (Dasso, 2008;Dawlaty et al, 2008;Eifler et al, 2018;Eifler and Vertegaal, 2015;Johansson et al, 2021;Liu et al, 2023;Ptak et al, 2021;Sridharan and Azuma, 2016;Yatskevich et al, 2021). During a normal mitosis, replicated sister chromatids segregate and move to the opposite poles of the mitotic spindle during anaphase, which is followed by spindle disassembly and cytokinesis that divides the daughter cells.…”

Section: Introductionmentioning

confidence: 99%

Aspergillus SUMOylation mutants have normal dynein function but exhibit chromatin bridges

Zhang

Qiu

Bieger

et al. 2023

Preprint

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Functions of protein SUMOylation remain incompletely understood in different cell types. The budding yeast SUMOylation machinery interacts with LIS1, a protein critical for dynein activation, but dynein-pathway components were not identified as SUMO-targets in the filamentous fungus Aspergillus nidulans. Via A. nidulans forward genetics, here we identified ubaBQ247*, a loss-of-function mutation in a SUMO-activation enzyme UbaB. Colonies of the ubaBQ247*, ∆ubaB and ∆sumO mutants looked similar and less healthy than the wild-type colony. In these mutants, about 10% of nuclei are connected by abnormal chromatin bridges, indicating the importance of SUMOylation in the completion of chromosome segregation. Nuclei connected by chromatin bridges are mostly in interphase, suggesting that these bridges do not prevent cell-cycle progression. UbaB-GFP localizes to interphase nuclei just like the previously studied SumO-GFP, but the nuclear signals disappear during mitosis when the nuclear pores are partially open, and the signals reappear after mitosis. The nuclear localization is consistent with many SUMO-targets being nuclear proteins, for example, topoisomerase II whose SUMOylation defect gives rise to chromatin bridges in mammalian cells. Unlike in mammalian cells, however, loss of SUMOylation in A. nidulans does not apparently affect the metaphase-to-anaphase transition, further highlighting differences in the requirements of SUMOylation in different cell types. Finally, loss of UbaB or SumO does not affect dynein- and LIS1-mediated early-endosome transport, indicating that SUMOylation is unnecessary for dynein or LIS1 function in A. nidulans.

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Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation

Abstract: Highlights d APC/C SUMOylation causes substantial rearrangement of the WHB domain of the APC2 subunit d The repositioned WHB domain prevents robust binding of the MCC to the APC/C

Cited by 22 publications

References 50 publications

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I

Lactate: A critical regulator of cell proliferation via anaphase promoting complex remodeling

Aspergillus SUMOylation mutants have normal dynein function but exhibit chromatin bridges

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