Molecular mechanisms of genetic recombination in bacteriophage

Linear Association Between Cellular DNA and Epstein-Barr Virus DNA in a Human Lymphoblastoid Cell Line

“…It would therefore appear that the mechanism of integration of EBV DNA is different from that found for SV40 (9) or adenoviruses (22), in that it does not involve DNA-DNA covalent linkage. Possibly, "linkers" of a different structure are present, e.g., short chains of RNA, or alternatively the cellular and viral DNA are only united by hydrogen bonds (20). It is of interest in this regard that about 10 covalently inserted ribonucleotide residues have recently been found in circular DNA molecules from human mitochondria (23), and that a small number of single-strand interruptions that are alkali labile are present in Herpes simplex DNA (24).…”

Bromodeoxyuridine-labeled bacteriophage T4D

“…The "dead" BUdR phages were found to have the same mean density in CsCl as plaque forming BUdR phages indicating that the inviability of BUdR phages is not due to large deficiencies of DNA. TOMIZAWA and ANRAKU (1964) found that the rlI A+ cistron was able to carry out its function in most BUdR phages. In the present experiments the production of intracellular phages and length of latent period were studied in restrictive bacteria employing rII A and rII B mutants as well as "early" and "late" umber mutants.…”

Biochemical Aspects of Genetics

“…On the contrary, breakage and reunion of DNA strands has been found in both cases. Tomizawa & Anraku (172,173) have investigated the molecular mechanism of recombination in phage T4. The addition of KCN to infected cells inhibited DNA synthesis without inhibiting recombination.…”