Molecular Mechanisms of Shigella Pathogenesis; Recent Advances

Pakbin, Babak; Brück, Wolfram M.; Brück, Thomas

doi:10.3390/ijms24032448

Cited by 24 publications

(12 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. enteritidis is among the most frequently encountered species, and is mainly found in chicken eggs [ 335 ]. Shigella dysenteriae is a common causative agent of diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome [ 336 ], with a wide arsenal of virulence factors at its disposal [ 336 , 337 ]. Antibiotic resistance is a concern in this case as well [ 338 ].…”

Section: Discussionmentioning

confidence: 99%

Use of Biomaterials in 3D Printing as a Solution to Microbial Infections in Arthroplasty and Osseous Reconstruction

Periferakis,

Troumpata

et al. 2024

Biomimetics

View full text Add to dashboard Cite

The incidence of microbial infections in orthopedic prosthetic surgeries is a perennial problem that increases morbidity and mortality, representing one of the major complications of such medical interventions. The emergence of novel technologies, especially 3D printing, represents a promising avenue of development for reducing the risk of such eventualities. There are already a host of biomaterials, suitable for 3D printing, that are being tested for antimicrobial properties when they are coated with bioactive compounds, such as antibiotics, or combined with hydrogels with antimicrobial and antioxidant properties, such as chitosan and metal nanoparticles, among others. The materials discussed in the context of this paper comprise beta-tricalcium phosphate (β-TCP), biphasic calcium phosphate (BCP), hydroxyapatite, lithium disilicate glass, polyetheretherketone (PEEK), poly(propylene fumarate) (PPF), poly(trimethylene carbonate) (PTMC), and zirconia. While the recent research results are promising, further development is required to address the increasing antibiotic resistance exhibited by several common pathogens, the potential for fungal infections, and the potential toxicity of some metal nanoparticles. Other solutions, like the incorporation of phytochemicals, should also be explored. Incorporating artificial intelligence (AI) in the development of certain orthopedic implants and the potential use of AI against bacterial infections might represent viable solutions to these problems. Finally, there are some legal considerations associated with the use of biomaterials and the widespread use of 3D printing, which must be taken into account.

show abstract

Section: Discussionmentioning

confidence: 99%

Use of Biomaterials in 3D Printing as a Solution to Microbial Infections in Arthroplasty and Osseous Reconstruction

Periferakis,

Troumpata

et al. 2024

Biomimetics

View full text Add to dashboard Cite

show abstract

“…Previous studies have demonstrated a substantial increase in resistance of Prevotella to several antibiotics (clindamycin, penicillin, cephalosporin, tetracycline, amoxicillin, and metronidazole), attributed to the presence of antimicrobial resistance genes or efflux pumps ( Sharma et al., 2022 ). The Shigella bacteria reside in the gastrointestinal tract and are the primary causative agents of bacillary diarrhea or shigellosis in humans ( Pakbin et al., 2023 ). The involvement of increased Shigella and decreased lysozyme in the pathogenesis of intestinal barrier dysfunction during acute necrotizing pancreatitis is evident ( Chen et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Oral administration of lysozyme protects against injury of ileum via modulating gut microbiota dysbiosis after severe traumatic brain injury

Yang,

Xi,

Yao

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

ObjectiveThe current study sought to clarify the role of lysozyme-regulated gut microbiota and explored the potential therapeutic effects of lysozyme on ileum injury induced by severe traumatic brain injury (sTBI) and bacterial pneumonia in vivo and in vitro experiments.MethodsMale 6–8-week-old specific pathogen-free (SPF) C57BL/6 mice were randomly divided into Normal group (N), Sham group (S), sTBI group (T), sTBI + or Lysozyme-treated group (L), Normal + Lysozyme group (NL) and Sham group + Lysozyme group (SL). At the day 7 after establishment of the model, mice were anesthetized and the samples were collected. The microbiota in lungs and fresh contents of the ileocecum were analyzed. Lungs and distal ileum were used to detect the degree of injury. The number of Paneth cells and the expression level of lysozyme were assessed. The bacterial translocation was determined. Intestinal organoids culture and co-coculture system was used to test whether lysozyme remodels the intestinal barrier through the gut microbiota.ResultsAfter oral administration of lysozyme, the intestinal microbiota is rebalanced, the composition of lung microbiota is restored, and translocation of intestinal bacteria is mitigated. Lysozyme administration reinstates lysozyme expression in Paneth cells, thereby reducing intestinal permeability, pathological score, apoptosis rate, and inflammation levels. The gut microbiota, including Oscillospira, Ruminococcus, Alistipes, Butyricicoccus, and Lactobacillus, play a crucial role in regulating and improving intestinal barrier damage and modulating Paneth cells in lysozyme-treated mice. A co-culture system comprising intestinal organoids and brain-derived proteins (BP), which demonstrated that the BP effectively downregulated the expression of lysozyme in intestinal organoids. However, supplementation of lysozyme to this co-culture system failed to restore its expression in intestinal organoids.ConclusionThe present study unveiled a virtuous cycle whereby oral administration of lysozyme restores Paneth cell’s function, mitigates intestinal injury and bacterial translocation through the remodeling of gut microbiota.

show abstract

“…dysenteriae causes illness by invading and damaging the epithelial cells that line the colon, ileum, and rectum in the gastrointestinal tract. This invasion disrupts the normal functioning of these cells, leading to symptoms such as diarrhoea, abdominal cramps, and fever [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

An integrated in-silico approach for drug target identification in human pathogen Shigella dysenteriae

Qureshi,

Basheer,

Sajjad

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Shigella dysenteriae, is a Gram-negative bacterium that emerged as the second most significant cause of bacillary dysentery. Antibiotic treatment is vital in lowering Shigella infection rates, yet the growing global resistance to broad-spectrum antibiotics poses a significant challenge. The persistent multidrug resistance of S. dysenteriae complicates its management and control. Hence, there is an urgent requirement to discover novel therapeutic targets and potent medications to prevent and treat this disease. Therefore, the integration of bioinformatics methods such as subtractive and comparative analysis provides a pathway to compute the pan-genome of S. dysenteriae. In our study, we analysed a dataset comprising 27 whole genomes. The S. dysenteriae strain SD197 was used as the reference for determining the core genome. Initially, our focus was directed towards the identification of the proteome of the core genome. Moreover, several filters were applied to the core genome, including assessments for non-host homology, protein essentiality, and virulence, in order to prioritize potential drug targets. Among these targets were Integration host factor subunit alpha and Tyrosine recombinase XerC. Furthermore, four drug-like compounds showing potential inhibitory effects against both target proteins were identified. Subsequently, molecular docking analysis was conducted involving these targets and the compounds. This initial study provides the list of novel targets against S. dysenteriae. Conclusively, future in vitro investigations could validate our in-silico findings and uncover potential therapeutic drugs for combating bacillary dysentery infection.

show abstract

Molecular Mechanisms of Shigella Pathogenesis; Recent Advances

Cited by 24 publications

References 105 publications

Use of Biomaterials in 3D Printing as a Solution to Microbial Infections in Arthroplasty and Osseous Reconstruction

Use of Biomaterials in 3D Printing as a Solution to Microbial Infections in Arthroplasty and Osseous Reconstruction

Oral administration of lysozyme protects against injury of ileum via modulating gut microbiota dysbiosis after severe traumatic brain injury

An integrated in-silico approach for drug target identification in human pathogen Shigella dysenteriae

Contact Info

Product

Resources

About