“…With the improvement of on-/off-target site specificity and efficiency and improved understanding of structures, mechanisms, clinical applications, and offtarget activities of genome editing systems, genome editing will become one of the clinical precision medicine strategies and multidisciplinary therapy strategies by integrating gene sequencing, clinical trans-omics, and single-cell biomedicine. 15 As parts of spatiotemporal molecular medicine, spatial transcriptomics, proteomics, metabolomics, and bioinformatics can be the important tools to evaluate whether the body is suitable for targeted gene editing by programmable endonucleases prior to the treatment and monitor how the systems' responses occur and how spatiotemporal controls might regulate functional switches or be regulated by gene editing associated alterations after the gene therapy. 16,17 Spatiotemporal molecular medicine requires the obvious contributions from rapid development of artificial intelligence, automatic robots, and computational and mathematical models.…”