Molecular mechanisms underlying antitumor activity of camel whey protein against multiple myeloma cells

Abstract

Treating drug-resistant cancer cells is a clinical challenge and it is also vital to screen for new cancer drugs. Multiple myeloma (MM) is a plasma cell clonal cancer that, despite many experimental therapeutics, remains incurable. In this study, two MM cell line lines U266 and RPMI 8226 were used to determine the impact of camel whey protein (CWP). The CWP IC 50 was calculated by MTT examination, while the flow cytometry analysis was used to investigate the chemotaxis responses of MM cells in relation to CXCL12 and the pro-apoptotic effect of CHP. MM cells were treated with CWP and Western blot analysis was used to determine the underlying molecular mechanisms. Dose and time based on the impact of CWP on the cell viability of MM cells with IC 50 of 50 μg/ml, without affecting the viability of normal healthy PBMCs. CWP reduced chemotaxis of MM cells significantly from the CXC chemokine ligand 12 (CXCL12). Using Western blot analysis, we found that CWP decreased the activation of AKT, mTOR, PLCβ3, NFαB and ERK, which was mechanistically mediated by CXCL12/CXCR4. In both U266 and RPMI 8226, CWP induced apoptosis by upregulating cytochrome C expression. In addition, CWP mediated the growth arrest of MM cells by robustly decreasing the expression of the anti-apoptotic Bcl-2 family members Bcl-2, Bcl-XL and Mcl-1. Conversely, the expression of pro-apoptotic Bcl-2 family members Bak, Bax and Bim was increased after treatment with CWP. Our data indicates CWP's therapeutic potential for MM cells.

Bee gomogenat rescues lymphoid organs from degeneration by regulating the crosstalk between apoptosis and autophagy in streptozotocin-induced diabetic mice

“…Then, HRP-conjugated species-matched secondary antibodies were used, the protein bands were detected with enhanced chemiluminescence (ECL, Super Signal West Pico Chemiluminescence Substrate, Perbio, Bezons, France), and the ECL signals were recorded using a LI-COR scanner. ImageJ software was used to quantify the protein band intensities as previously described (Badr and Mohany 2011 ; Badr et al 2021 ).…”

The improvement potential of camel milk whey as a natural remedy in comparison with Rivastigmine chitosan-loaded nanoparticles in aluminum chloride induced Alzheimer-like disease in rats

“…CWP is deemed a powerful natural antioxidant since it improves immunity, lowers oxidative stress, and elevates antioxidants. It encompasses various active constituents, like lactoglobulins, immunoglobulins, lactoferrin (LF), lactoperoxidase, lysozyme, and α-lactalbumin [12]. Moreover, CWP reduces oxidative stress, protects neurons, and improves the neurobehavior of diabetic mice [13].…”