Molecular Mechanisms Underlying Drug Resistance of the MCF7/ADR Breast Cancer Cell Line

Quantitative site- and structure-specific N-glycoproteomics characterization of differential N-glycosylation in MCF-7/ADR cancer stem cells

“…In 2017, Xing et al found that ALDH1 and ABCG2 were enhanced in primary foci and metastatic lymph node from patients with triple-negative breast cancer with qRT-PCR, western blotting and MTT assay of mRNA expression, protein expression and proliferation of MDA-MB-231 cells, respectively; and the authors proposed that ALDH1 and ABCG2 may affect the drug resistance [13]. In 2018, Bogusha et al found that induction of the epithelial-mesenchymal transition process made bigger contribution to the drug resistance of MCF-7/ADR cells than the ABC transporter's overexpression, because differential expression of vimentin is much higher than that of P-gp as measured by immunofluorescent staining with antibodies [14].…”

N-Glycoproteomics Study of Putative N-Glycoprotein Biomarkers of Drug Resistance in MCF-7/ADR Cells

“…MCF-7 cell line is a very common breast cancer cell model for the study of MDR mechanisms. The MCF-7/ADR cell line is resistant to the chemotherapeutic agent doxorubicin, and some MDRrelated proteins are also identified in this cell line (Bogush et al 2018). In the study of immunofluorescent staining with antibodies, differential expression of mesenchymal protein vimentin in MCF-7/ADR cells relative to MCF-7 cells was found to be much higher than that of P-gp, which indicated that mesenchymal phenotype of epithelial cells contributed much more to MDR than the overexpression of ABC transporter (Bogush et al 2018).…”

“…The MCF-7/ADR cell line is resistant to the chemotherapeutic agent doxorubicin, and some MDRrelated proteins are also identified in this cell line (Bogush et al 2018). In the study of immunofluorescent staining with antibodies, differential expression of mesenchymal protein vimentin in MCF-7/ADR cells relative to MCF-7 cells was found to be much higher than that of P-gp, which indicated that mesenchymal phenotype of epithelial cells contributed much more to MDR than the overexpression of ABC transporter (Bogush et al 2018). Down-regulation of proto-oncogene c-fos in MCF-7/ADR cells led to decreased expression of P-gp and enhanced apoptosis with increased expression of B-cell lymphoma 2 (Bcl-2) as well as decreased expression of apoptosis regulator Bax, cellular tumor antigen p53 and Bcl-2-binding component 3, isoforms 1/2 (PUMA) (Shi et al 2013).…”