1982
DOI: 10.1016/0167-4889(82)90025-8
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Molecular mobilities and the lowered osmolality of the chromaffin granule aqueous phase

Abstract: Carbon-13 spin-lattice relaxation times, T1, have been measured in whole adrenal medullary tissue slices, in suspensions of isolated chromaffin granules, in the reconcentrated chromaffin granule lysate, and in various model solutions containing catecholamines. ATP, chromogranins and Ca2+. Reorientational correlation times have been calculated at 10 degrees C using T1 data and nuclear Overhauser enhancements for protonated carbons on both catecholamines and nucleotides. Correlation times in all media are relati… Show more

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Cited by 16 publications
(10 citation statements)
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“…1□ S ); (ii) NO effects were observed even on pre-fusioned granules obtained eliciting secretion under hypertonic conditions, and secretion was elicited only on return to isotonic conditions and (iii) in these prefusioned granules, NO prevented the CA leakage. Chromogranin A, ATP, and Ca 2ϩ have been implicated in the intragranular Donnan complexation of CA (15)(16)(17). To date, this process has been considered to be only a passive mechanism for keeping the intragranular solutes isotonic with the cytoplasm (35).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…1□ S ); (ii) NO effects were observed even on pre-fusioned granules obtained eliciting secretion under hypertonic conditions, and secretion was elicited only on return to isotonic conditions and (iii) in these prefusioned granules, NO prevented the CA leakage. Chromogranin A, ATP, and Ca 2ϩ have been implicated in the intragranular Donnan complexation of CA (15)(16)(17). To date, this process has been considered to be only a passive mechanism for keeping the intragranular solutes isotonic with the cytoplasm (35).…”
Section: Discussionmentioning
confidence: 99%
“…Complexation of intragranular substances will reduce the osmotic forces, thereby preventing granule lysis (15)(16)(17)(18). No mechanisms are known at present that regulate this intragranular matrix complex.…”
mentioning
confidence: 99%
“…The time course of the late step of exocytosis might be altered by a different mechanism, including modification of the affinity of catecholamines for the intragranular matrix (Schroeder et al, 1996). In this case, chromogranin A, ATP, and Ca 2ϩ have been implicated in the intragranular Donnan complex of catecholamines (Kopell and Westhead, 1982;Sen and Sharp, 1982;Helle et al, 1990), and therefore, slight modifications in the conformation of chromogranin A might account for large changes in its affinity for catecholamines, with consequent changes in release (Leszczyszyn et al, 1990;Schroeder et al, 1996, Rahamimoff andFernández, 1997). Then, the possibility existed that fragmentation of chromogranin A to catestatin might modify catecholamine release by altering the last step of the exocytotic process.…”
mentioning
confidence: 99%
“…It is unlikely that decreased solute interactions within chromaffin granules could account for the necessary increase in osmotic equivalents. The granule interior is an extremely nonideal solution because of interactions between the major osmoticants, epinephrine and ATP (Ko-pel1 and Westhead, 1982;Sen and Sharp, 1982). The solute interactions are relatively insensitive to changes in pH, divalent ions, NaCl, or KCI.…”
Section: Relationship Of the Findings To Exocytosis From Intact Cellsmentioning
confidence: 99%