Molecular Modification of Co-Trimoxazole, Tetracycline, Aspirin in Combination With Psidium Guajava and Syzygium Aromaticum for Treatment of Clinical Resistant Salmonella Infection : Case Study Kakuri Nigeria.

Abstract

For centuries plant extract has been used for treatments of various diseases, medicinal plants has also been the source of many active components of prescribe drugs. Drug resistant Salmonella infections has been of growing concern in Nigeria due to regular dosing by self-medication. The aim of this work is to identify a novel compounds with potentially improved antimicrobial activities by molecular modification of ineffective antibiotics and non-antibiotics using aqueous plant extract for the treatment of resistant salmonella spp at lower concentration. Co-trimoxazole, Tetracycline and Aspirin at 10 µg/mL were synthetically combine with water extract of Cloves and Guava leaves in three different stages using concentrated sulphuric acid and sodium hydroxide, 31 antimicrobial disk were prepared and tested on salmonella spp. Scl, a one-step combination of co-trimoxazole with clove extract, Ac2 a two-step combinatorial synthesis of aspirin with clove extract, Ag2 a two-step combinatorial synthesis of Aspirin with guava leave extract were all susceptible to resistant salmonella spp with clear zones of inhibition at 10 µg/mL better than Ciprofloxacin at 20 µg/mL. no inhibition shown by A10, T10, S10, Gu, Cl, Agu, Tgu, Sgu, Tcl, Scl, Ag1, Tg1, Sg1, Ac1, Tc1, Tg2, Sg2, Tc2, Sc2, Ag3, Tg3, Sg3, Ac3, Tc3 and Sc3. Aspirin was the best precursor drug which combine synthetically with both clove and guava extract to give a carrier product with desired antimicrobial properties, hence the need for isolation purification and further clinical trials.