Molecular Organization of Acyl Lipids in Photosynthetic Membranes of Higher Plants

Siegenthaler, Paul‐André

doi:10.1007/0-306-48087-5_7

Cited by 43 publications

(52 citation statements)

References 128 publications

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“…PG is the only phospholipid in thylakoid membranes. The role of glycerolipids in the function of thylakoid membranes has been studied in vitro and several of these studies were summarized in a review (Siegenthaler 1998). It was also demonstrated that tolerance to chilling stress is closely connected with the fatty acid unsaturation of plant membrane lipids (Somerville and Browse 1991;Moon et al 1995;Sakurai et al 2003;Szalontai et al 2003).…”

Section: Introductionmentioning

confidence: 98%

Overexpression of glycerol-3-phosphate acyltransferase gene improves chilling tolerance in tomato

Sui

Zhao

et al. 2007

Planta

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A tomato (Lycopersicon esculentum Mill.) glycerol-3-phosphate acyltransferase gene (LeGPAT) was isolated. The deduced amino acid sequence revealed that LeGPAT contained four acyltransferase domains, showing high identities with GPAT in other plant species. A GFP fusion protein of LeGPAT was targeted to chloroplast in cowpea mesophyll protoplast. RNA gel blot showed that the mRNA accumulation of LeGPAT in the wild type (WT) was induced by chilling temperature. Higher expression levels were observed when tomato leaves were exposed to 4 degrees C for 4 h. RNA gel and western blot analysis confirmed that the sense gene LeGPAT was transferred into the tomato genome and overexpressed under the control of 35S-CaMV. Although tomato is classified as a chilling-sensitive plant, LeGPAT exhibited selectivity to 18:1 over 16:0. Overexpression of LeGPAT increased total activity of LeGPAT and cis-unsaturated fatty acids in PG in thylakoid membrane. Chilling treatment induced less ion leakage from the transgenic plants than from the WT. The photosynthetic rate and the maximal photochemical efficiency of PS II (Fv/Fm) in transgenic plants decreased more slowly during chilling stress and recovered faster than in WT under optimal conditions. The oxidizable P700 in both WT and transgenic plants decreased obviously at chilling temperature under low irradiance, but the oxidizable P700 recovered faster in transgenic plants than in the WT. These results indicate that overexpression of LeGPAT increased the levels of PG cis-unsaturated fatty acids in thylakoid membrane, which was beneficial for the recovery of chilling-induced PS I photoinhibition in tomato.

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Section: Introductionmentioning

confidence: 98%

Overexpression of glycerol-3-phosphate acyltransferase gene improves chilling tolerance in tomato

Sui

Zhao

et al. 2007

Planta

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“…The role of glycerolipids in the function of thylakoid membranes has been studied in vitro and the important studies were summarized in a review (Siegenthaler, 1998). However, the findings obtained in those studies do not provide direct evidence for a role of glycerolipids in photosynthesis and cannot be directly applied to the function of glycerolipids in vivo.…”

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confidence: 98%

“…They are mainly composed of glycerolipids forming lipid bilayers and of protein complexes involved in photosynthetic electron transport and energy conversion (Siegenthaler, 1998). The lipid composition of thylakoid membranes is highly conserved among eukaryotic plants and cyanobacterial strains, and is distinct from that of other membranes, which contain phospholipids as the major glycerolipids (Block et al, 1983;Murata and Nishida, 1987;Wada and Murata, 1998).…”

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confidence: 99%

Direct Evidence for Requirement of Phosphatidylglycerol in Photosystem II of Photosynthesis

et al. 2000

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Phosphatidylglycerol (PG) is considered to play an important role in the ordered assembly and structural maintenance of the photosynthetic apparatus in thylakoid membranes. However, its function in photosynthesis remains poorly understood. In this study we have identified a pgsA gene of Synechocystis sp. PCC6803 that encodes a PG phosphate synthase involved in the biosynthesis of PG. A disruption of the pgsA gene allowed us to manipulate the content of PG in thylakoid membranes and to investigate the function of PG in photosynthesis. The obtained pgsA mutant could grow only in the medium containing PG, and the photosynthetic activity of the pgsA mutant dramatically decreased with a concomitant decrease of PG content in thylakoid membranes when the cells grown in the presence of PG were transferred to the medium without PG. This decrease of photosynthetic activity was attributed to the decrease of photosystem (PS)II activity, but not to the decrease in PSI activity. These findings demonstrate that PG is essential for growth of Synechocystis sp. PCC6803 and provide the first direct evidence that PG plays an important role in PSII.

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“…MGDG is the main thylakoid membrane lipid of vascular plants, followed by the bilayer-forming digalactosyldiacylglycerol (DGDG) as the second most abundant lipid (Joyard et al, 1998;Siegenthaler, 1998). The lipid classes of vascular plants are comparable to those in isolated diatom thylakoids .…”

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confidence: 99%

“…With regard to the concentration of charged lipids, however, significant differences exist. Diatom thylakoids can contain more than 40% of their total membrane lipids in the form of the negatively charged lipids sulfoquinovosyldiacylgylcerol (SQDG) and phosphatidylglycerol (PG; , whereas in plant thylakoid membranes, these two lipid species amount to only 20% or less (Murata and Siegenthaler, 1998;Siegenthaler, 1998). Thereby, SQDG surpasses DGDG as the second most abundant lipid .…”

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confidence: 99%

Evidence for the Existence of One Antenna-Associated, Lipid-Dissolved and Two Protein-Bound Pools of Diadinoxanthin Cycle Pigments in Diatoms

et al. 2010

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We studied the localization of diadinoxanthin cycle pigments in the diatoms Cyclotella meneghiniana and Phaeodactylum tricornutum. Isolation of pigment protein complexes revealed that the majority of high-light-synthesized diadinoxanthin and diatoxanthin is associated with the fucoxanthin chlorophyll protein (FCP) complexes. The characterization of intact cells, thylakoid membranes, and pigment protein complexes by absorption and low-temperature fluorescence spectroscopy showed that the FCPs contain certain amounts of protein-bound diadinoxanthin cycle pigments, which are not significantly different in high-light and low-light cultures. The largest part of high-light-formed diadinoxanthin cycle pigments, however, is not bound to antenna apoproteins but located in a lipid shield around the FCPs, which is copurified with the complexes. This lipid shield is primarily composed of the thylakoid membrane lipid monogalactosyldiacylglycerol. We also show that the photosystem I (PSI) fraction contains a tightly connected FCP complex that is enriched in protein-bound diadinoxanthin cycle pigments. The peripheral FCP and the FCP associated with PSI are composed of different apoproteins. Tandem mass spectrometry analysis revealed that the peripheral FCP is composed mainly of the light-harvesting complex protein Lhcf and also significant amounts of Lhcr. The PSI fraction, on the other hand, shows an enrichment of Lhcr proteins, which are thus responsible for the diadinoxanthin cycle pigment binding. The existence of lipid-dissolved and protein-bound diadinoxanthin cycle pigments in the peripheral antenna and in PSI is discussed with respect to different specific functions of the xanthophylls.

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Molecular Organization of Acyl Lipids in Photosynthetic Membranes of Higher Plants

Cited by 43 publications

References 128 publications

Overexpression of glycerol-3-phosphate acyltransferase gene improves chilling tolerance in tomato

Overexpression of glycerol-3-phosphate acyltransferase gene improves chilling tolerance in tomato

Direct Evidence for Requirement of Phosphatidylglycerol in Photosystem II of Photosynthesis

Evidence for the Existence of One Antenna-Associated, Lipid-Dissolved and Two Protein-Bound Pools of Diadinoxanthin Cycle Pigments in Diatoms

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