Molecular Phenotyping and Genomic Characterization of a Novel Neuroactive Bacterium Strain, Lactobacillus murinus HU-1

Lebovitz, Yeonwoo; Theus, Michelle H.

doi:10.3389/fphar.2019.01162

Cited by 6 publications

(3 citation statements)

References 49 publications

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“…In contrast, the abundance of L. murinus was significantly increased in ISO-O rats at both ages tested. Importantly, administration of L. murinus HU-1 was sufficient to rescue behavioral deficits and brain region-specific microglial activation observed in maternal microbiome dysbiosis-reared murine offspring [29]. Hence, the high proportion of L. murinus in male ISO-O might act as a compensatory mechanism downstream of the social behavior deficit previously reported in ISO-O rats [6].…”

Section: Discussionmentioning

confidence: 69%

Parental Social Isolation during Adolescence Alters Gut Microbiome in Rat Male Offspring

Siddi,

Cosentino,

Tamburini

et al. 2024

Biomolecules

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Previous work from our laboratory demonstrated that parental stress, induced by social isolation starting at puberty, leads to behavioral, endocrine, and biochemical changes in the male, but not female, offspring (ISO-O) of Sprague-Dawley rats. Here, we report alterations in the gut microbiota composition of ISO-O vs. grouped-housed offspring (GH-O), although all animals received the same diet and were housed in the same conditions. Analysis of bacterial communities by next-generation sequencing (NGS) of 16S rRNA gene revealed alterations at family and order levels within the main phyla of Bacteroides, Proteobacteria, and Firmicutes, including an almost total deficit in Limosilactobacillus reuteri (formerly Lactobacillus reuteri) and a significant increase in Ligilactobacillus murinus (formerly Lactobacillus murinus). In addition, we found an increase in the relative abundance of Rhodospirillales and Clostridiales in the families of Lachnospiraceae and Ruminococcaceae, and Bacteroidales in the family of Prevotellaceae. Furthermore, we examined plasma levels of the proinflammatory cytokines interleukin-1-beta and tumor necrosis factor-alpha, which did not differ between the two groups, while corticosterone concentrations were significantly increased in ISO-O rats. Our findings suggest that adverse environmental conditions experienced by parents may have an impact on the likelihood of disease development in the subsequent generations.

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Section: Discussionmentioning

confidence: 69%

Parental Social Isolation during Adolescence Alters Gut Microbiome in Rat Male Offspring

Siddi,

Cosentino,

Tamburini

et al. 2024

Biomolecules

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“…L. murinus relatively and significantly increased in the treatment group. Lebovitz & Theus, 2019 have reported the applications of L. murinus from various studies (Lebovitz & Theus, 2019) and outlined that it has a beneficial effect on the host, including antimicrobial production, antagonist against pathogens, intestinal barrier, and can be developed as a potential probiotic. B. thetaiotaomicron helps in Carbohydrate metabolism, lipid metabolism, and enervates the production of proinflammatory cytokines, and finally helps to strengthen the host‐microbiome ecosystem (Jandhyala et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…that may limit the growth of pathogens to protect intestinal mucosa by strengthening toxic oxidation(Vieco-saiz et al, 2019), that could be another cause for the decrease in species richness in the encapsulated group. L. murinus relatively and significantly increased in the treatment group Lebovitz & Theus, 2019. have reported the applications of L. murinus from various studies(Lebovitz & Theus, 2019) and outlined that it has a beneficial effect on the host, including antimicrobial production, antagonist against pathogens, intestinal barrier, and can be developed as a potential probiotic.…”

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confidence: 99%

Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota

Gyawali

Guiyuan²,

et al. 2023

Food Science & Nutrition

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The beneficial effect of probiotics on host health is impaired due to the substantial loss of survivability during gastric transit caused by small intestinal enzymes and bile acids. Encapsulation helps to preserve the probiotics species from severe environmental factors. Lactobacillus paracasei, highly sensitive probiotic species to gastric acid, was encapsulated with polyacrylate resin. C57BL/6 male mice were equally divided into three groups; control group was fed with basal diet without any additives, the un‐encapsulated group was fed with 0.1% of a mixture of encapsulating material and L. paracasei, and encapsulated group was fed with 0.1% encapsulated L. paracasei (microcapsule) for 4 weeks. The result showed elevated fecal moisture percentage in the encapsulated group, but not in the un‐encapsulated group. Further study showed that the ratio of villus height to crypt depth in the small intestine was significantly higher compared to un‐encapsulated and the control group. Microencapsulated probiotics also remarkably increased intestinal mucin and secretory immunoglobulin A (sIgA) concentration, intestinal MUC‐2, and tight junction protein mRNA expression levels improving the intestinal barrier function of mice. In addition, microcapsules also reduced proinflammatory factor mRNA expression, while considerably increasing anti‐inflammatory factor mRNA expression. Microbiota metabolites, fecal LPS (Lipopolysaccharide) were downregulated, and acetate and lactate were upraised compared to control. Furthermore, glutathione peroxidase (GSH‐Px) and TAOC levels were increased and Malondialdehyde (MDA) was decreased improving antioxidant capacity. Microflora and bioinformatic predictive analysis of feces showed that encapsulated probiotics remarkably increased Lactobacillus proportions. Mice's intestinal health can thus be improved by using microencapsulated probiotics.

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The Gut Microbiome

Francella

Foster

2021

Burger's Medicinal Chemistry and Drug Discovery

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In the past decade, there has been a remarkable growth in the interest and enthusiasm for microbiome research. The microbiome includes bacteria, viruses, fungi, and parasites; our microbes cover all parts of our body. Researchers from many disciplines are now considering the role of microbiota and its relationship to development, physical health, and mental health. The multidisciplinary nature of ongoing research efforts is advancing our knowledge base at a fast pace. The current attention to tool development for analysis beyond bacterial composition will help scientists interpret the relationship between microbes, health, and disease. This article provides an introduction to host–microbe interactions based on animal and clinical research focused on the gut microbiome.

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Molecular Phenotyping and Genomic Characterization of a Novel Neuroactive Bacterium Strain, Lactobacillus murinus HU-1

Cited by 6 publications

References 49 publications

Parental Social Isolation during Adolescence Alters Gut Microbiome in Rat Male Offspring

Parental Social Isolation during Adolescence Alters Gut Microbiome in Rat Male Offspring

Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota

The Gut Microbiome

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