Molecular Phylogeny and Morphological Distinctions of Two Popular Bivalves,<i>Ctenoides scaber</i>and<i>Ctenoides mitis</i>

Dougherty, Lindsey F.; Li, Jingchun

doi:10.1155/2017/1624014

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…All stages were examined on an Olympus SZX16 stereoscopic microscope (Olympus Life Science, Center Valley, PA, USA). To further confirm morphological identification, ticks homogenates were subjected to molecular identification by amplifying the highly conserved 708 bp mitochondrial Cytochrome Oxidase I gene (COI) [ 15 ]. The nucleotide accession numbers were MT876643, MT876644, and MT876645.…”

Section: Methodsmentioning

confidence: 99%

Tick-Borne Pathogens Shape the Native Microbiome Within Tick Vectors

et al. 2020

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Ticks are blood-feeding arthropods and transmit a variety of medically important viral, bacterial, protozoan pathogens to animals and humans. Ticks also harbor a diverse community of microbes linked to their biological processes, such as hematophagy, and hence affect vector competence. The interactions between bacterial and/or protozoan pathogens and the tick microbiome is a black-box, and therefore we tested the hypothesis that the presence of a protozoan or bacterial pathogen will alter the microbial composition within a tick. Hence, this study was designed to define the microbial composition of two tick species, Hyalomma (H.) anatolicum and Rhipicephalus (R.) microplus. We used a combination of PCR based pathogen (Anaplasma marginale and Theileria species) and symbiont (Wolbachia species) identification followed by metagenomic sequencing and comparison of the microbial communities in PCR positive and negative ticks. A total of 1786 operational taxonomic units was identified representing 25 phyla, 50 classes, and 342 genera. The phylum Proteobacteria, Firmicutes, Actinobacteriota, and Bacteroidota were the most represented bacteria group. Alpha and beta diversity were not significantly affected in the presence or absence of Theileria sp. and A. marginale as see with H. anatolicum ticks. Interestingly, bacterial communities were significantly reduced in Theileria sp. infected R. microplus ticks, while also exhibiting a significant reduction in microbial richness and evenness. Putting these observations together, we referred to the effect the presence of Theileria sp. has on R. microplus a “pathogen-induced dysbiosis”. We also identify the presence of Plasmodium falciparum, the causative agent of human malaria from the microbiome of both H. anatolicum and R. microplus ticks. These findings support the presence of a “pathogen-induced dysbiosis” within the tick and further validation experiments are required to investigate how they are important in the vector competence of ticks. Understanding the mechanism of “pathogen-induced dysbiosis” on tick microbial composition may aid the discovery of intervention strategies for the control of emerging tick-borne infections.

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Section: Methodsmentioning

confidence: 99%

Tick-Borne Pathogens Shape the Native Microbiome Within Tick Vectors

et al. 2020

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“…Scallops are a commercially important shellfish in worldwide, particularly in the Caribbean region [ 22 ]. A relatively abundant scallop in Caribbean coral reef ecosystems is Ctenoides scaber (Born, 1778), commonly called the “flame scallop”, due to that they possess a red mantle and extensive tentacles [ 23 , 24 ]. Natural populations are found in shallow Atlantic coastal waters from North Carolina in United States of America to the northeastern coast of Venezuela, and their reproductive cycle and population dynamics as well as their potential for aquaculture are known [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%