Molecular phylogeny of Burkholderia pseudomallei

Multilocus Sequence Typing of Historical Burkholderia pseudomallei Isolates Collected in Southeast Asia from 1964 to 1967 Provides Insight into the Epidemiology of Melioidosis

“…The majority of the isolates from the southern region of Thailand were assigned novel STs, indicating a great amount of genotypic diversity among environmental B. pseudomallei isolates from this region. The ST diversity encountered for southern Thai isolates in this study contrasts with previous research, where ribotyping analysis demonstrated a lack of genetic diversity among B. pseudomallei isolates from southern Thailand (23). eBURST analysis of the MLST data predicts that the isolates of the historical collection form three groups of related STs with many outlying, or unlinked, STs when analyzed separately as a population.…”

“…eBURST analysis of the MLST data predicts that the isolates of the historical collection form three groups of related STs with many outlying, or unlinked, STs when analyzed separately as a population. This is a discontinuous pattern among isolates of a relatively clonal species as demonstrated by ribotyping (23). The epidemiological significance of the historical isolates is demonstrated upon the addition of the MLST data generated during this study to the current MLST data of the B. pseudomallei MLST database.…”

“…The population structure of B. pseudomallei is clonal at the rRNA level, although clinical isolates of B. pseudomallei exhibit genetic diversity and are thus excellent candidates for MLST (23). It was therefore expected that historical isolates of the B. pseudomallei strain collection would exhibit tremendous diversity according to MLST analysis.…”

Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae

“…Because of the rapid turnaround time, low concentration of starting target DNA needed, and specificity, PCR assays are becoming a popular means for bacterial identification and differentiation in many clinical and public health laboratories (Pitt et al, 2000). There are several reports in the literature that describe PCR-based assays for identifying B. pseudomallei; however, none of the assays are currently being used for melioidosis diagnosis (Bauernfeind et al, 1998;Gee et al, 2003;Hagen et al, 2002;Holden et al, 2004;Lee et al, 2005;Sprague et al, 2002;Tanpiboonsak et al, 2004;Thibault et al, 2004;Tomaso et al, 2004).…”

Comparison of Rapid, Automated Ribotyping and DNA Macrorestriction Analysis ofBurkholderia pseudomallei

“…The technical demands and time required to complete a single analysis restrict ribotyping to centers with a Burkholderia research interest. The more accessible method of DNA macrorestriction analysis (pulsed-field gel electrophoresis [PFGE]) is widely used for molecular typing of B. pseudomallei (6,7,13,16). Both methods have been used to investigate suspected Burkholderia sp.…”