Molecular phylogeny of Capripoxviruses based on major immunodominant protein (P32) reveals circulation of host specific sheeppox and goatpox viruses in small ruminants of India

Sumana, K.; Yogisharadhya, Revanaiah; Shivachandra, Sathish Bhadravati; Mothay, Dipti; Apsana, R.; Saminathan, Mani; Basavaraj, S.; Reddy, G.B. Manjunatha

doi:10.1016/j.meegid.2020.104472

Cited by 9 publications

(9 citation statements)

References 37 publications

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“…Histologic skin lesions are characterized by dermal edema and cellularity with variable numbers of "sheeppox cells," histiocytic like cells with large vacuolated nuclei and poorly-defined eosinophilic cytoplasmic inclusions [594]. A variety of serologic and molecular diagnostics are available, with some molecular approaches capable of rapid detection and differentiation between GP and SP [588,[595][596][597][598][599][600][601][602].…”

Section: Sheeppox and Goatpoxmentioning

confidence: 99%

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Clemmons

Alfson

Dutton

2021

Animals

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Animals provide food and other critical resources to most of the global population. As such, diseases of animals can cause dire consequences, especially disease with high rates of morbidity or mortality. Transboundary animal diseases (TADs) are highly contagious or transmissible, epidemic diseases, with the potential to spread rapidly across the globe and the potential to cause substantial socioeconomic and public health consequences. Transboundary animal diseases can threaten the global food supply, reduce the availability of non-food animal products, or cause the loss of human productivity or life. Further, TADs result in socioeconomic consequences from costs of control or preventative measures, and from trade restrictions. A greater understanding of the transmission, spread, and pathogenesis of these diseases is required. Further work is also needed to improve the efficacy and cost of both diagnostics and vaccines. This review aims to give a broad overview of 17 TADs, providing researchers and veterinarians with a current, succinct resource of salient details regarding these significant diseases. For each disease, we provide a synopsis of the disease and its status, species and geographic areas affected, a summary of in vitro or in vivo research models, and when available, information regarding prevention or treatment.

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Section: Sheeppox and Goatpoxmentioning

confidence: 99%

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Clemmons

Alfson

Dutton

2021

Animals

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“…However, the vaccine efficacy is not consistent rather varies with quality, efficacy, safety, side effects and even price (Tuppurainen et al., 2021 ). Moreover, there are the issues of improper characterization of circulating wild strains as well as viral vaccine strains in terms of host specificity, vaccination failures and safety concerns with capripox vaccinations (Sumana et al., 2020 ). Moreover, the administration of heterologous vaccinations in Turkey was not able to stop LSD outbreaks, indicating that the success of the vaccine is not adequate, necessitating the development of new vaccine candidate for control and prevention of LSD.…”

Section: Introductionmentioning

confidence: 99%

Development of membrane protein‐based vaccine against lumpy skin disease virus (LSDV) using immunoinformatic tools

Salauddin,

Kayesh,

Ahammed

et al. 2024

Veterinary Medicine & Sci

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IntroductionLumpy skin disease, an economically significant bovine illness, is now found in previously unheard‐of geographic regions. Vaccination is one of the most important ways to stop its further spread.AimTherefore, in this study, we applied advanced immunoinformatics approaches to design and develop an effective lumpy skin disease virus (LSDV) vaccine.MethodsThe membrane glycoprotein was selected for prediction of the different B‐ and T‐cell epitopes by using the immune epitope database. The selected B‐ and T‐cell epitopes were combined with the appropriate linkers and adjuvant resulted in a vaccine chimera construct. Bioinformatics tools were used to predict, refine and validate the 2D, 3D structures and for molecular docking with toll‐like receptor 4 using different servers. The constructed vaccine candidate was further processed on the basis of antigenicity, allergenicity, solubility, different physiochemical properties and molecular docking scores.ResultsThe in silico immune simulation induced significant response for immune cells. In silico cloning and codon optimization were performed to express the vaccine candidate in Escherichia coli. This study highlights a good signal for the design of a peptide‐based LSDV vaccine.ConclusionThus, the present findings may indicate that the engineered multi‐epitope vaccine is structurally stable and can induce a strong immune response, which should help in developing an effective vaccine towards controlling LSDV infection.

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“… 2008 ) and, subsequently, based on whole genome sequencing, P32 gene-based PCR and sequencing (Sumana et al. 2020a , 2020b ), RPO30 gene-based conventional PCR, and G-protein-coupled chemokine receptor (GPCR) gene-based quantitative PCR (Lamien et al. 2011 ; Santhamani et al.…”

Section: Introductionmentioning

confidence: 99%

Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India

Manjunatha Reddy,

Sumana,

Yogisharadhya

et al. 2024

Veterinary Quarterly

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Sheeppox and goatpox are transboundary viral diseases of sheep and goats that cause significant economic losses to small and marginal farmers worldwide, including India. Members of the genus Capripoxvirus (CaPV), namely Sheeppox virus (SPPV), Goatpox virus (GTPV), and Lumpy skin disease virus (LSDV), are antigenically similar, and species differentiation can only be accomplished using molecular approaches. The present study aimed to understand the molecular epidemiology and host specificity of SPPV and GTPV circulating in India through sequencing and structural analysis of the RNA polymerase subunit-30 kDa ( RPO30 ) gene. A total of 29 field isolates from sheep ( n = 19) and goats ( n = 10) belonging to different geographical regions of India during the period: Year 2015 to 2023, were analyzed based on the sequence and structure of the full-length RPO30 gene/protein. Phylogenetically, all the CaPV isolates were separated into three major clusters: SPPV, GTPV, and LSDV. Multiple sequence alignment revealed a highly conserved RPO30 gene, with a stretch of 21 nucleotide deletion in all SPPV isolates. Additionally, the RPO30 gene of the Indian SPPV and GTPV isolates possessed several species-specific conserved signature residues/motifs that could act as genotyping markers. Secondary structure analysis of the RPO30 protein showed four α-helices, two loops, and three turns, similar to that of the E4L protein of vaccinia virus (VACV). All the isolates in the present study exhibited host preferences across different states of India. Therefore, in order to protect vulnerable small ruminants from poxviral infections, it is recommended to take into consideration a homologous vaccination strategy.

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Molecular phylogeny of Capripoxviruses based on major immunodominant protein (P32) reveals circulation of host specific sheeppox and goatpox viruses in small ruminants of India

Cited by 9 publications

References 37 publications

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Development of membrane protein‐based vaccine against lumpy skin disease virus (LSDV) using immunoinformatic tools

Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India

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