Molecular Phylogeny of &lt;I&gt;Ophiopogon&lt;/I&gt; (Asparagaceae) Inferred from Nuclear and Plastid DNA Sequences

Wang, Guangyan; Meng, Ying; Huang, Jinling; Yang, Yongping

doi:10.1600/036364414x682201

Cited by 10 publications

(22 citation statements)

References 28 publications

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“…species, and nine accessions representing six Peliosanthes Andrews species. Three Maianthemum F. H. Wigg species and one Convallaria L. species were chosen as outgroups based on a previous study (Wang et al, ). Collection information and GenBank accession numbers are provided in Table .…”

Section: Methodsmentioning

confidence: 99%

“…Most sequences used in this study were from our previous data (Wang et al, ). Sequences for several species were extracted from 15 mg desiccated leaf tissue using the CTAB method of Doyle & Doyle ().…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we followed the guidelines for obtaining reliable ITS regions in Ophiopogon proposed by Wang et al (), with the primers ITS4 and ITS5 (White et al, ). If amplification of the entire ITS region was unsuccessful, internal primers ITS2 and ITS3 were used in the combinations ITS2/ITSmF and ITS3/ITS4 to obtain two shorter, overlapping fragments.…”

Section: Methodsmentioning

confidence: 99%

“…as a source of tuberous roots (Chen & Tamura, ). Based on phylogenetic analysis of nuclear ribosomal internal transcribed spacers (ITS) and plastids ( psbA‐trnH , matK , rbcL , and trnL‐F ), Wang et al () better supported the classification of Wang & Tang (). Molecular classification accepted most of the previously recognized species, and some species were classified into sections: O. lancangensis H. Li & Y. P. Yang; O. multiflorus Y. Wan; O. yunnanensis S. C. Chen; O. reversus C. C. Huang; O. longibracteatus H. Li & Y. P. Yang (sect.…”

Section: Introductionmentioning

confidence: 98%

“…In this study, we investigate the historical biogeography of Ophiopogon using a Bayesian uncorrelated log‐normal and relaxed molecular clock approach, based on our previous phylogenetic framework (Wang et al, ). The ancestral area of Ophiopogon and subsequent dispersal routes are inferred, and the current distribution of the genus is explained using molecular dating results.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Hypothesizing the origin, migration routes, and distribution patterns of Ophiopogon (Asparagaceae) in East and Southeast Asia

Wang

Yang

2018

J of Sytematics Evolution

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Ophiopogon Ker-Gawl. (Ophiopogoneae, Asparagaceae) is a genus containing approximately 65 species distributed in East and Southeast Asia. The biogeographical history within the area is still poorly known. Divergence time estimation was used to infer the biogeographic history of Ophiopogon based on nuclear ribosomal internal transcribed spacer data from 33 Ophiopogon species, three Liriope Lour. species, and six Peliosanthes Andrews species. The ancestral area and dispersal routes of Ophiopogon were inferred using the Bayesian binary method. Ophiopogon motouensis S. C. Chen was a transitional taxon. Ophiopogon was estimated to have originated in Southwest China during the middle Miocene (11.74 Ma). Five migrations were hypothesized to explain the expansion of Ophiopogon

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hypothesizing the origin, migration routes, and distribution patterns of Ophiopogon (Asparagaceae) in East and Southeast Asia

Wang

Yang

2018

J of Sytematics Evolution

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Plastid genome sequencing, identification of nuclear SNP markers, and quality assessment of medicinal rhizomatous herb Polygonatum odoratum (Asparagaceae) cultivars

Lee

Chen

et al. 2021

Ecology and Evolution

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Polygonatum odoratum (Mill.) Druce (Asparagaceae, Asparagales) is a widely cultivated medicinal herb in China. However, this useful herb is understudied despite being known as a medicinal resource with top grade medical and edible properties since long. In this study, P. odoratum and four cultivars were investigated. The variations in morphological characteristics and vegetative phases of each cultivar were observed. For genetic aspect, the plastid genome of P. odoratum varies in length from 154,569 bp to 155,491 bp, containing a large single‐copy region of 83,486–84,459 bp, a small single‐copy region of 18,292–18,471 bp, and two inverted repeats of 26,302–26,370 bp. A total of 131 genes were predicted, including 85 protein‐coding, 38 tRNA, and eight rRNA genes. Genome comparisons revealed a slight variation in the sequence across the five accessions, but two highly variable regions (trnC‐petN and rpl32‐trnL) were detected when comparing the four different cultivars. For the RAD‐seq markers, a total of 33.64 Gb of clean data, with an average value of 1.08 Gb per sample, were analyzed for the presence of single nucleotide polymorphisms (SNPs). Well‐resolved phylogenies of the P. odoratum cultivars are constructed; the nonmonophyletic relationship in the plastome‐based phylogenetic trees, yet monophyletic form in the RAD‐based linkage map suggested possibility of hybrid cultivar for P. odoratum “Dazhu” (GDDZ), which was further supported by morphological observations. Quality assessment based on the standards of the Chinese Pharmacopoeia on Polygonati Odorati Rhizoma (POR) on the four cultivars used in this study recorded that PORs from P. odoratum “Zhongzhu” (GDZZ) met the minimum criteria for the acceptance as raw material for medicinal drug production. This study has provided insights on the morphological variations, genetic background, and medicinal qualities of P. odoratum cultivars that could be explored for future genetic improvement as well as breeding programs of P. odoratum for POR production.

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Genome size variation among and within Ophiopogoneae species by flow cytometric analysis

2017

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Genome size variation in a taxonomic group reflects evolutionary processes. DNA contents of Ophiopogoneae (40 populations of 31 species) were estimated by flow cytometry. Ploidy levels of Ophiopogon (ten species), Liriope (two species), and Peliosanthes (three species) were determined based on the DNA contents. The genus Peliosanthes showed significant larger genome sizes than Ophiopogon (P \ 0.01), and Ophiopogon also significant larger than Liriope (P \ 0.05). Intraspecific variation in genome size was mainly chromosome difference. The ITS sequence phylogeny splitted Ophiopogon into two clades, clade I comprising sect. Ophiopogon with diploids and tetraploids, and clade II including transitional species and sects. Ophiopogon and Peliosanthoides with diploids. The trend seemed to increase in genome size from Ophiopogon sect. Peliosanthoides (13.45 pg) to Ophiopogon sect. Ophiopogon (14.27 pg). Polyploidization may be evolutionary direction of Ophiopogon. Our results also suggested that the 'increase' hypothesis for genome size evolutionary may hold true in the genus Ophiopogon.

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Molecular Phylogeny of <I>Ophiopogon</I> (Asparagaceae) Inferred from Nuclear and Plastid DNA Sequences

Cited by 10 publications

References 28 publications

Hypothesizing the origin, migration routes, and distribution patterns of Ophiopogon (Asparagaceae) in East and Southeast Asia

Hypothesizing the origin, migration routes, and distribution patterns of Ophiopogon (Asparagaceae) in East and Southeast Asia

Plastid genome sequencing, identification of nuclear SNP markers, and quality assessment of medicinal rhizomatous herb Polygonatum odoratum (Asparagaceae) cultivars

Genome size variation among and within Ophiopogoneae species by flow cytometric analysis

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