Molecular phylogeny of Megalobatrachonema (Nematoda: Ascaridida), with description of a new species based on morphological and molecular evidence

Chen, Hui-Xia; Zhang, Luping; Sinsch, Ulrich; Scheid, Patrick; Balczun, Carsten; Li, L.

doi:10.1016/j.meegid.2020.104172

Cited by 8 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present knowledge of the molecular phylogeny of Cosmocercoidea/Cosmocercidae is still very limited. To date, several studies [8][9][10][11] have provided molecular phylogenetic analyses to solve the systematic status of some genera in Cosmocercoidea using different genetic data. However, due to the paucity and inaccessibility of suitable material of Cosmocercoidea/Cosmocercidae for genetic analysis, all of these molecular phylogenetic studies have included only small numbers of representatives of these taxa.…”

Section: Introductionmentioning

confidence: 99%

Description of a new species of Aplectana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa

Chen

et al. 2021

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

Background Nematodes of the family Cosmocercidae (Ascaridomorpha: Cosmocercoidea) are mainly parasitic in the digestive tract of various amphibians and reptiles worldwide. However, our knowledge of the molecular phylogeny of the Cosmocercidae is still far from comprehensive. The phylogenetic relationships between Cosmocercidae and the other two families, Atractidae and Kathlaniidae, in the superfamily Cosmocercoidea are still under debate. Moreover, the systematic position of some genera within Cosmocercidae remains unclear. Methods Nematodes collected from Polypedates megacephalus (Hallowell) (Anura: Rhacophoridae) were identified using morphological (light and scanning electron microscopy) and molecular methods [sequencing the small ribosomal DNA (18S), internal transcribed spacer 1 (ITS-1), large ribosomal DNA (28S) and mitochondrial cytochrome c oxidase subunit 1 (cox1) target regions]. Phylogenetic analyses of cosmocercoid nematodes using 18S + 28S sequence data were performed to clarify the phylogenetic relationships of the Cosmocercidae, Atractidae and Kathlaniidae in the Cosmocercoidea and the systematic position of the genus Aplectana in Cosmocercidae. Results Morphological and genetic evidence supported the hypothesis that the nematode specimens collected from P. megacephalus represent a new species of Aplectana (Cosmocercoidea: Cosmocercidae). Our phylogenetic results revealed that the Cosmocercidae is a monophyletic group, but not the basal group in Cosmocercoidea as in the traditional classification. The Kathlaniidae is a paraphyletic group because the subfamily Cruziinae within Kathlaniidae (including only the genus Cruzia) formed a seperate lineage. Phylogenetic analyses also showed that the genus Aplectana has a closer relationship to the genus Cosmocerca in Cosmocercidae. Conclusions Our phylogenetic results suggested that the subfamily Cruziinae should be moved from the hitherto-defined family Kathlaniidae and elevated as a separate family, and the genus Cosmocerca is closely related to the genus Aplectana in the family Cosmocercidae. The present study provided a basic molecular phylogenetic framework for the superfamily Cosmocercoidea based on 18S + 28S sequence data for the first time to our knowledge. Moreover, a new species, A. xishuangbannaensis n. sp., was described using integrative approach. Graphical abstract

show abstract

Section: Introductionmentioning

confidence: 99%

Description of a new species of Aplectana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa

Chen

et al. 2021

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

show abstract

“…LC605539-LC605541). The BLAST search showed the highest similarity (98%) with a sequence of Megalobatrachonema terdentatum (Linstow, 1890) (Nematoda: Kathlaniidae) (MN444706, Chen et al, 2020). The haplotype of larval Falcaustra sp.…”

Section: Molecular Studymentioning

confidence: 99%

The First Survey for Helminths Parasitic in Hybrid and Introduced Giant Salamanders, Genus Andrias (Amphibia: Caudata: Cryptobranchidae) in Kyoto, Japan

Tsuchida

Urabe

Nishikawa

2021

Current Herpetology

View full text Add to dashboard Cite

show abstract

“…Our present knowledge of the molecular phylogeny of the Cosmocercoidea/ Cosmocercidae is still very limited. To date, several studies [8][9][10][11] provided molecular phylogenetic analyses to solve the systematic status of some genera using different genetic data. However, due to the paucity and inaccessibility of suitable material of Cosmocercoidea / Cosmocercidae for genetic analysis, all of these molecular phylogenetic studies have included only small numbers of representatives of the Cosmocercoidea / Cosmocercidae.…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of the family Cosmocercidae (Nematoda: Ascaridida), with description of a new species of Aplectana using an integrative approach

Chen¹,

Gu²,

Ni³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background Nematodes of the family Cosmocercidae (Ascaridida: Cosmocercoidea) are mainly parasitic in the digest tract of various amphibians and reptiles worldwide. However, our knowledge of the molecular phylogeny of the Cosmocercidae is still far from comprehensive. The phylogenetic relationships of the Cosmocercidae and the other two families Atractidae and Kathlaniidae in the superfamily Cosmocercoidea, are still under debate. Moreover, the systematic position of some genera in Cosmocercidae remains unclear. Methods Nematodes collected from Polypedates megacephalus (Hallowell) (Anura: Rhacophoridae) were identified using morphological methods (light and scanning electron microscopy) and molecular approaches [sequencing and analyzing the small ribosomal DNA (18S), internal transcribed spacer 1 (ITS-1), large ribosomal DNA (28S) and mitochondrial cytochrome c oxidase subunit 1 (cox1) target regions]. Phylogenetic analyses of cosmocercoid nematodes using 18S + 28S sequence data were performed to clarify the phylogenetic relationships of the Cosmocercidae, Atractidae and Kathlaniidae in the Cosmocercoidea, and the systematic position of the genus Aplectana in Cosmocercidae. Results Morphological and genetic evidence supported that the nematode specimens collected from P. megacephalus represents a new species of Aplectana (Cosmocercoidea: Cosmocercidae). Our phylogenetic results revealed that the Cosmocercidae is a monophyletic group, but not the basal group in Cosmocercoidea as the traditional classification. The Kathlaniidae is a paraphyletic group, and the subfamily Cruziinae (including only the genus Cruzia) formed a sister relationship to the Cosmocercidae. Phylogenetic analyses also showed that the genus Aplectana has closer relationship to the genus Cosmocerca in the Cosmocercidae. Conclusions Our molecular phylogenetic results supported that the subfamily Cruziinae should be moved out from the hitherto-defined family Kathlaniidae and elevated to a separate family, and the genus Cosmocerca has closer relationship to the genus Aplectana in the family Cosmocercidae, Our present study provided the basic molecular phylogenetic framework for the superfamily Cosmocercoidea based on 18S + 28S sequence data for the first time. Moreover, a new species of Aplectana, A. xishuangbannaensis n. sp., was described using an integrative approach.

show abstract

Molecular phylogeny of Megalobatrachonema (Nematoda: Ascaridida), with description of a new species based on morphological and molecular evidence

Cited by 8 publications

References 17 publications

Description of a new species of Aplectana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa

Description of a new species of Aplectana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa

The First Survey for Helminths Parasitic in Hybrid and Introduced Giant Salamanders, Genus Andrias (Amphibia: Caudata: Cryptobranchidae) in Kyoto, Japan

Molecular phylogeny of the family Cosmocercidae (Nematoda: Ascaridida), with description of a new species of Aplectana using an integrative approach

Contact Info

Product

Resources

About