Molecular phylogeny of Ostracoda (Crustacea) inferred from 18S ribosomal DNA sequences: implication for its origin and diversification

Molecular Systematics of Tanaidacea (Crustacea: Peracarida) Based on 18S Sequence Data, with an Amendment of Suborder/Superfamily-Level Classification

“…(Spears et al, 2005). Primers 18S-F2 and 18S-F3 were designed by Yamaguchi and Endo (2003). Other primers were designed by using sequences obtained during this study, taking into account the location of variable regions assessed from the secondary structure of the honeybee 18S sequence (Gillespie et al, 2006), and were checked for their adequacies with Primer3Plus (Untergasser et al, 2007).…”

An exceptionally preserved myodocopid ostracod from the Silurian of Herefordshire, UK

“…The monophyly of (myodocope and podocope) ostracods has been questioned in molecular phylogenies (e.g. Yamaguchi & Endo 2003;Regier et al 2005) and supported by the use of a morphological approach (e.g. Cohen et al 1998; see Horne et al 2005).…”

On the phylogenetic position of Pseudophilomedinae within Sarsielloidea (Ostracoda, Myodocopida), with a description of one new Harbansus from Ningaloo Reef and redescription of H. paucichelatus from Yucatan

“…DNA was extracted using LaboPass Tissue Mini extraction kit, following the manufacturer protocol. Fragments of nuclear 18S rDNA (on average 1,800 bp) were amplified using the primer pair F1/R9 from Yamaguchi and Endo (2003), fragments of 28S rDNA were amplified using the primer pares dd/ff, ee/mm, and vv/xx from Hills and Dixon (1991), and for mitochondrial COI sequences, we used standard Folmer primers (Folmer et al 1994). For all gene fragments, the PCR was done in a TaKaRa PCR Thermal Cycler Dice in 25 lL volume containing: 5 lL of the DNA template, 2.5 lL, 109 ExTaq Buffer, 0.25 lL of TaKaRa Ex Taq (5 U/lL), 2 lL of dNDTP Mixture (2.5 mM each), 1 lL each primer, and 13.25 lL distilled H 2 O.…”