Molecular prevalence of<i>Toxoplasma gondii</i><scp>DNA</scp>in goats’ milk and seroprevalence in Northwest Tunisia

Amairia, Safa; Rouatbi, Mariem; Rjeibi, Mohamed Ridha; Nouasri, Hanen; Sassi, Limam; Mhadhbi, Moez; Gharbi, Mohamed

doi:10.1002/vms3.29

Cited by 35 publications

(22 citation statements)

References 36 publications

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“…Many different molecular techniques are used for diagnosis of T. gondii in milk samples as conventional PCR [ 18 , 19 ], nested PCR [ 23 ], PCR-RFLP technique [ 24 ], and qPCR [ 25 , 26 ]. qPCR assay is the most rapid, accurate, and effective molecular technique that can be used for Toxoplasma detection [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt

Saad¹,

Hussein²,

Ewida³

2018

Vet World

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Background and Aim:Toxoplasmosis is a worldwide zoonotic disease with harmful effects on animal and human health. Ingestion of contaminated raw milk has been suggested as a vehicle for transmission of Toxoplasma gondii to human. The present study was performed for the detection of T. gondii in raw milk samples of goat, sheep, and camel in Upper Egypt using two different techniques (enzyme-linked immunosorbent assay [ELISA] and quantitative polymerase chain reaction [qPCR]).Materials and Methods:This study was conducted to determine the T. gondii prevalence using ELISA and qPCR in raw goat, sheep, and camels milk (30 samples for each) collected from different locations in Upper Egypt.Results:T. gondii IgG antibodies were detected in 90.0, 60.0, and 3.33% of goat, sheep, and camel milk samples, respectively. From the positive samples of T. gondii IgG, the parasitic DNA was detected only in two examined milk samples, one of them was present in goat milk sample and another one was found in sheep milk sample. On the other hand, the parasite was not detected in camels’ milk samples.Conclusion:These results concluded that the raw milk was contaminated by T. gondii tachyzoites which could be a source of human infection. Restricted hygienic programs should be implemented in the animal farms to decrease the risk of milk contamination by this parasite.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt

Saad¹,

Hussein²,

Ewida³

2018

Vet World

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“…Along with sheep, T. gondii infection was studied in other species such as goats and horses [10,33]. To assess the risk of toxoplasmosis transmission through contaminated food, one study was conducted in goat milk samples, reporting a molecular infection rate of 7.8% [9]. Detecting parasite DNA in milk does not mean that the parasite is alive; further studies are needed to determine parasite viability.…”

Section: Toxoplasma Gondii Infection In Algeriamentioning

confidence: 99%

Toxoplasma gondii infection and toxoplasmosis in North Africa: a review

Rouatbi¹,

Amairia²,

Amdouni³

et al. 2019

Parasite

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Toxoplasmosis is an important zoonosis caused by an obligate intracellular parasitic protozoan, Toxoplasma gondii. The disease is distributed worldwide and can affect all warm-blooded vertebrates, including humans. The present review aimed to collect, compile and summarize the data on the prevalence of T. gondii infection in humans and animals in the five North African countries (Morocco, Algeria, Tunisia, Libya and Egypt). Published data from national and international databases were used. Distribution patterns and risk factors for T. gondii infection are discussed, focusing on biotic and abiotic factors. This review is a comprehensive epidemiological analysis of T. gondii infection in North Africa and will therefore be a useful tool for researchers. It can also be used to propose or enhance appropriate national toxoplasmosis control programs.

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“…; Amairia et al . ). It has been shown that insemination, using experimentally contaminated fresh semen with different T. gondii tachyzoites concentrations was able to transmit infection to sheep, indicating the possibility of a sexual transmission route (Bezerra et al .…”

Section: Introductionmentioning

confidence: 97%

Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia

et al. 2016

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Toxoplasmosis is a worldwide zoonosis caused by the parasitic protozoan Toxoplasma gondii. It can infect all warm‐blooded vertebrate species and causes abortions and birth defects in pregnant women and pregnant ewes. The objective of this study was to estimate the prevalence of infection with T. gondii in sheep meat in the region of Sidi Bouzid (central Tunisia) and Beja (northern Tunisia), the realization of a descriptive study of risk factors and the phylogenetic analyses of T. gondii. Neck muscle samples were obtained from 174 ewes and ewe lamb slaughtered in Sidi Bouzid and 150 lambs slaughtered in Beja. DNA was extracted from the samples using the Wizard® genomic DNA purification kit. A nested PCR using two pairs of primers (NN 1 and NN2, Tg‐NP1 and Tg‐NP2) were used to detect infection with T. gondii, which was then confirmed by sequencing. Eight T. gondii amplicons were sequenced (accession number KT896498) and deposited in GenBank. The T. gondii amplicons showed 97–100% identities with GenBank sequences. A phylogenetic tree was then constructed. The nested PCR detected T. gondii DNA in 31% of animals tested in Sidi Bouzid and 32% of lambs tested in Beja. No significant difference in the prevalence of T. gondii infection was established between the two tested regions. In both regions, no significant variation of the infection depending on age, breed and locality was found.

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Molecular prevalence ofToxoplasma gondiiDNAin goats’ milk and seroprevalence in Northwest Tunisia

Cited by 35 publications

References 36 publications

Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt

Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt

Toxoplasma gondii infection and toxoplasmosis in North Africa: a review

Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia

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