Molecular Profile of Sensitization to Dermatophagoides pteronyssinus Dust Mite in Portugal

Limão, R; Santos, Amélia Spínola; Araújo, Luís; Cosme, Joana; Inácio, Filipe; Tomáz, Elza; Ferrão, Anabela; Santos, Norma; Sokolova, A; Morête, Ana; Falcão, Helena; Cunha, Liliana; Ferreira, Ana Reis; Bras, Alexandra Le; Ribeiro, F. Ramôa; Lozoya, Carlos; Pinto, Paula; Prates, Sara; Plácido, José Luís; Coimbra, Alice; Taborda-Barata, Luís; Santos, Maria Conceição Pereira; Barbosa, Miguel; Pineda, Fernando

doi:10.18176/jiaci.0533

Cited by 9 publications

(12 citation statements)

References 1 publication

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“…Even though sIgE-array platforms have included a wide spectrum of HDM allergens (8,14), only Der p 1, Der p 2, Der p 23, and Der p 10 (tropomyosin) are currently available for the component-resolved diagnosis of HDM allergy on ImmunoCAP. Our results, using InmunoCAP in the largest sample published to date, are similar to those previously reported in terms of prevalence of sensitization (6,8,14,15,17) but we report a high rate of monosensitization to Der p 23 (6,8,(15)(16)(17)(18)20,21). The clinical relevance of Der p 23 is only partially defined, and the prevalence and relevance of the exclusive sensitization to it has received little attention so far.…”

Section: Discussionsupporting

confidence: 90%

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Der p 23 sensitization in patients with house dust mite respiratory allergy

Romero-Sánchez¹,

Otero²,

González-Rivas³

et al. 2024

Eur Ann Allergy Clin Immunol

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Background: The sensitization profile of patients allergic to house dust mites (HDM) and its molecular diagnosis may determine treatment and evolution of the disease. The present study investigates the prevalence of Der p 23 sensitization and its relation to asthma in a population of HDM-allergic patients.Methods: We conducted a cross-sectional study of 891 patients with HDM allergy with symptoms of rhinitis and 52.1% of them with asthma. Total and specific IgE (sIgE) was measured against Dermatophagoides pteronyssinus and its molecular components (Der p 1, Der p 2 and Der p 23) and the storage mite Lepidoglyphus destructor using ImmunoCAP. Prevalence of sensitization and levels of sIgE were analysed according to asthma diagnosis and asthma severity.Results: Der p 23 was the predominant allergen in this population (83.7%) but IgE levels were lower than those of sIgE to Der p 1 and Der p 2. A good correlation was found between sIgE to Der p 23 and the other allergens. A total of 8.2% patients were monosensitized to Der p 23. Asthma was more frequent in patients with positive sIgE against Der p 23 than in patients without this sensitization (52.8% vs 42.8%, p=0.027). A tendency to increase both total IgE and sIgE was observed in relation to the severity of asthma from intermittent mild asthma to persistent moderate asthma but a substantial decrease in total IgE and sIgE was detected in more severe asthmatics. Conclusion: Der p 23 might be a prevalent allergen in regions with high rates of HDM exposure. Even though sIgE levels against this allergen are usually low, its presence could increase the risk of asthma.

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Section: Discussionsupporting

confidence: 90%

“…House dust mites (HDM) are a major cause of respiratory allergy and of perennial asthma worldwide. Der p 23, a gut-derived peritrophin present in the outer membrane of mite feces, has been recognized as a major allergen (3,8,15,16,(17)(18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

Der p 23 sensitization in patients with house dust mite respiratory allergy

Romero-Sánchez¹,

Otero²,

González-Rivas³

et al. 2024

Eur Ann Allergy Clin Immunol

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“…House dust mites (HDM) are an important source of perennial allergens that play a key role in the development of high-impact allergic diseases such as rhinitis and allergic asthma (RA, AA) or atopic dermatitis (AD) that have a great impact on quality of life and associated healthcare costs [1][2][3]. HDM allergens are very powerful and act by activating both adaptive and innate immune systems, so controlling their exposure is a challenge [4].…”

Section: Introductionmentioning

confidence: 99%

“…Geographic heterogeneity indicates the need for greater standardization in epidemiological research since exposure to allergens depends on multiple factors such as climate and mite microhabitats within the domestic environment. Humidity is a critical factor for its development [2]. The adaptive propagation of HDM suggests that it thrives in the life conditions preferred by humans [4].…”

Section: Introductionmentioning

confidence: 99%

“…As previously described, in Galicia Dpt has a significant clinical importance as a main trigger of asthma, allergic rhinitis, and AD [5]. Although Cheyletus species, Euroglyphus maynei, Lepidoglyphus destructor (Ldt), and Chortoglyphus arcuatus or Blomia tropicalis (Blot) are also common (the latter more significant in subtropical climates such as the Canary Islands) [1][2][3][4][5]. Some studies place Blot as a cause of respiratory allergy, and Dpt and Dfar as a key feature in children with AD; increasing the risk of asthma and allergic rhinitis in dual sensitization [1].…”

Section: Introductionmentioning

confidence: 99%

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Serodominance Profile in a Dust Mite Complex Region

López-Rodríguez

Rial

Esteban-Gorgojo³

et al. 2022

Int Arch Allergy Immunol

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Background/Objective: The objective of this study was to describe the molecular sensitization profile of mite allergy in an area with a high environmental exposure of house dust mites (HDM) and storage mites. Methods: Skin prick tests were performed with standardized extracts (DIATER, Madrid, Spain). A specific commercial molecular panel (MADx) for Dermatophagoides pteronyssinus (Dpt), Dermatophagoides farinae (Dfar), Lepidoglyphus destructor (Ldt), Tyrophagus putrescentiae (Tput), and Blomia tropicalis (Blot) was correlated with clinical parameters in Galician (northwestern of Spain) HDM allergic patients. Results: Fifty patients (60% female) met the inclusion and exclusion criteria. All of the patient’s present rhinitis (50), 28% (14) rhinitis and asthma, and 18% (9) atopic dermatitis (AD). Hundred patients had a positive prick test for Dpt, followed by Dfar (92%), Ldt and Tput (74%), and Blot (68%). More than 50% recognized specific IgE for Der p 1, Der p 2, reaching 86% in the case of Der p 23. No statistically significant differences in IgE levels were found between patients with/without asthma and those with mild or moderate-severe rhinitis. Der p 7 was higher among rhinitis patients (p value 0.05). AD relative risk (RR) was increased in patients sensitized to Der f 2, Der p 2, and Der p 23. Der p 10 decreases the risk to have AD (RR 0.80). Conclusion: The evaluation of IgE results in a comprehensive panel of allergens allows differentiation of serological reactivity profiles with their clinical expression, to perform an optimal management. Improvements in component resolved diagnosis and more research on the clinical relevance of mite allergens are needed to achieve a genuine diagnosis leading to specific immunotherapy.

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Sensitization profiles to house dust mite Dermatophagoides pteronyssinus molecular allergens in the Lithuanian population: Understanding allergic sensitization patterns

Biliute,

Miskinyte,

Miskiniene

et al. 2024

Clinical & Translational All

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BackgroundHouse dust mite (HDM) allergy is a prevalent global health concern, with varying sensitization profiles observed across populations. We aimed to provide a comprehensive assessment of molecular allergen sensitization patterns in the Lithuanian population, with a focus on Dermatophagoides pteronyssinus (Der p), and investigate patterns of concomitant reactivity among different allergens to enhance the accuracy of HDM allergy diagnostics.MethodsA comprehensive analysis of 1520 patient test results in Lithuania from 2020 to 2022 was performed. Sensitization patterns to major (Der p 1, Der p 2, and Der p 23) and minor (Der p 5, Der p 7, and Der p 21) Der p allergen components were described using molecular‐based diagnostics. Additionally, we investigated sensitization to allergen components from other allergen sources, including tropomyosins (Der p 10, Per a 7, Pen m 1, Ani s 3, Blo t 10) and arginine kinases (Pen m 2, Bla g 9, Der p 20).ResultsThis study reveals a high prevalence of HDM sensitization in Lithuania ‐ 481 individuals (45.38% of the sensitized group) exhibited sensitization to at least one Der p allergen component. Importantly, within the sensitized group, 37.21% of patients were sensitized to Der p 5, Der p 7, or Der p 21 in addition to major allergenic components. Distinct sensitization patterns were observed across different age groups, indicating the influence of age‐related factors. Furthermore, we confirmed cross‐reactivity between Der p 5 and Blo t 5 as well as between Der p 21 and Blo t 21, emphasizing the clinical relevance of these associations. We also highlighted the complexity of sensitization patterns among tropomyosins and arginine kinases.ConclusionThis study provides valuable insights into HDM allergy sensitization profiles in Lithuania, emphasizing the importance of considering major and minor HDM allergen components for accurate diagnosis and management of HDM‐related allergic diseases. Differences between populations and age‐related factors impact sensitization patterns. Understanding concomitant reactivity among allergens, such as Der p 5 and Blo t 5, Der p 21 and Blo t 21, tropomyosins, and arginine kinases, is crucial for improving diagnostic strategies and developing targeted interventions for allergic individuals.

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Molecular Profile of Sensitization to Dermatophagoides pteronyssinus Dust Mite in Portugal

Cited by 9 publications

References 1 publication

Der p 23 sensitization in patients with house dust mite respiratory allergy

Der p 23 sensitization in patients with house dust mite respiratory allergy

Serodominance Profile in a Dust Mite Complex Region

Sensitization profiles to house dust mite Dermatophagoides pteronyssinus molecular allergens in the Lithuanian population: Understanding allergic sensitization patterns

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