Molecular profiling and genetic diversity of mungbean (<i>Vigna radiata</i>L.) genotypes using ISSR and SSR markers

Sao, Manoj Kumar; Nair, S.K.; Verulkar, Satish; Saxena, R. C.; Nanda, H.C.

doi:10.5958/0976-058x.2015.00068.2

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…Similar results reported by earlier Tonk et al, (2011) in Hypericum perforatum L. using RAPD primers. In contrast to our results the higher PIC (0.907) reported by Sao et al, (2015) in molecular profiling of mungbean using ISSR and SSR markers. But lower PIC values (0.22 to 0.36) reported by Kameli et al, (2013) in Satureja species using 10 ISSR primers and also it was ranged from 0.13 to 0.20 reported by Satyanarayana et al, (2010) in Mucuna using AFLP primers.…”

Section: Results and Discussion Issr Polymorphismcontrasting

confidence: 99%

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

Chinapolaiah

Bindu

Khadke

et al. 2018

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Velvet bean is an important medicinal legume, its seeds are prominent source of L-Dopa. The present investigation on genetic diversity assessment of 58 germplasm of velvet bean by using 11 ISSR markers. Out of 63 amplified products 59 were showed polymorphism and 4 were monomorphic with an average of 5.7 bands amplified per primer. According to band statistics and efficiency parameters showed the primers UBC 827, UBC 834 and UBC 836 were more efficient. The highest genetic similarity values (0.90) were observed between IIHR MP 102 and IIHR MP 74-3. In dendrogram germplasm grouped into two major clusters at 63 per cent similarity. Among the germplasm, IIHR Selection 4, IIHR Selection 10, IIHR MP 9, IC 33243 and IIHR MP 7 were found to be distinctly divergent, can be used in the further breeding programme.

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Section: Results and Discussion Issr Polymorphismcontrasting

confidence: 99%

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

Chinapolaiah

Bindu

Khadke

et al. 2018

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“…The grain/seeds of the cultivars Sulata, Pant-U-31, Kalindi, Sarada and Gautam were almost alike. So, any combination of these SSR primers can be used for ascertaining the seed purity or admixture and genetic purity at any stage of the growth period (LESTARI et al, 2014;NATH et al, 2017;SANGHANI et al, 2015;SAO et al, 2015). DNA fingerprinting through its high precision in identifying crop varieties, holds considerable promise as a reliable tool of intellectual property protection of crop varieties and germplasm.…”

Section: Results and Disscussionmentioning

confidence: 99%

Development of new microsatellite markers for DNA fingerprinting pattern of black gram (Vigna mungo L. Hepper) and green gram (Vigna radiate L. Wilckzek)

Panigrahi

Mohanty

et al. 2020

Genetika

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SSR markers are considered to be the most ideal marker for genetic studies because they are multi-allelic, abundant, randomly and widely distributed throughout the genome, co-dominant that could differentiate plants with homozygous or heterozygous alleles, simple to assay, highly reliable, reproducible. Microsatellite markers are highly polymorphic and informative and could be successfully used for genome analysis in black gram & green gram. Microsatellite markers were used to evaluate genetic diversity in 17 indigenous cultivars of pulse crops (11 cultivars of green gram and 6 cultivars of black gram respectively). They are subjected to variability analysis with 26 microsatellite markers for identification efficient primers to conclude the nature of molecular diversity present among the pulses. The SSR primer G228 showed 63.63% of polymorphism followed by MB-SSR 238 (45.45%) and G006 (36.36%). The 12 microsatellite markers produced 15.90 % polymorphism with banding ranged up to 7 with an average of 2.3 polymorphic banding patterns per SSR primer. Similarly for black gram, three random microsatellite primers G006 (50%) and G166 and G204 (33.33%) revealed considerable DNA polymorphism. The 14 random SSR primers produced 8.33% of polymorphism with banding ranged up to three with an average of 1.28 polymorphic banding pattern per SSR primer. The Distinguish Power (D), Polymorphism Information Content (PIC) value and Marker Index (MI) values revealed some SSR primers like G006, G204 and G166 can alone amplified distinct banding pattern, where as a combination of (G228+G006), (G228+G304) for green gram and the combination (G006+G166) can be used for black gram for ascertaining genetic diversity at any stage of crop growth period for green gram or black gram. From the present study we can conclude that selective microsatellite markers are highly polymorphic, informative and easily reproducible, which can be successfully used either as single or with combination for molecular characterization of crop species belonging to Vigna species.

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“…The diversity in plants provide an opportunity for developing new varieties and improved cultivars with desirable characteristics (Govindaraj et al, 2015;Saleh et al, 2016). A number of methodologies exist for the assessment of genetic diversity in maize, those are (i) morphological characterization (Ristic et al, 2014) (ii) biochemical characterization that uses electrophoresis to detect allelic variants of enzymes at gene level (Govindaraj et al, 2015) (iii) pedigree that employ the extraction of genealogical information (Drinic et al, 2012) and (iv) DNA molecular analyses (Sao et al, 2015) such as Simple Sequence Repeats (SSR), Restriction Fragment Length Polymorphism (RFLP) (Mondini et al, 2009). …”

Section: Maize Lethal Necrosis Disease (Mlnd)mentioning

confidence: 99%

Genetic diversity of maize accessions for maizelethal necrosis disease resistance

Ndakidemi

Sospeter

Ndunguru

et al. 2016

IJARe

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Maize is among the most preferred crop in Tanzania and other parts of the world. However, its production has been facing a number of challenges. Maize Lethal Necrosis Disease (MLND) is a new challenge in Eastern Africa. The control of MLND is said to be complicated as it is caused by a combination of more than one virus viz. Maize Chlorotic Mottle Virus (MCMV) and Sugarcane Mosaic Virus (SCMV). Stakeholders agree that the priority is to identify MLND resistant maize varieties. Genetic diversity provides the source of traits required against maize production challenges such as MLND. The study of genetic diversity in maize accessions often involves characterizing morphological plant characteristics as well as molecular marker techniques to study variation at DNA level. This review explores different literatures that address the importance of genetic diversity and the possibility of generating information towards obtaining potential materials against maize challenges and MLND in particular.

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Molecular profiling and genetic diversity of mungbean (Vigna radiataL.) genotypes using ISSR and SSR markers

Cited by 4 publications

References 6 publications

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

Development of new microsatellite markers for DNA fingerprinting pattern of black gram (Vigna mungo L. Hepper) and green gram (Vigna radiate L. Wilckzek)

Genetic diversity of maize accessions for maizelethal necrosis disease resistance

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