Molecular profiling of fungal assemblages in the healthy and infected roots of Decalepis arayalpathra (J. Joseph &amp; V. Chandras) Venter, an endemic and endangered ethnomedicinal plant from Western Ghats, India

Premalatha, K.; Gokul, Sivaraman; Kumar, Amit; Mishra, Prachi; Mishra, Pooja; Ravikumar, K.; Kalra, Alok

doi:10.1007/s13213-014-0919-7

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…Thus, it can be seen that several bacteria phyla dominant for safflower also colonize and function well in healthy humans. In terms of the fungal species, Ascomycota and Basidiomycota taxa are abundant in the as‐studied samples, whereas Penicillium and Aspergillus were restricted to healthy roots and acted as the promising entophytic communities against the root rot disease [34]. Penicillin, the first antibiotic derived from Penicillium belonging to Ascomycota phylum, has been used successfully in treating pathogenic microbe‐infected humans.…”

Section: Discussionmentioning

confidence: 99%

Study on the correlation between soil microbial diversity and ambient environmental factors influencing the safflower distribution in Xinjiang

et al. 2020

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The effects of soil microbial properties and physiographical factors on safflower distributions in the main safflower plantations of Xinjiang province in China were studied. This study may help determine the basis of the environmental factors for evaluating the geoherbalism of this medicinal plant. The soil microbial biodiversity in the bulk soil and rhizosphere of safflower at different growth stages and from different sampling plots were characterized by analyzing the environmental DNAs in the samples. With general primers targeting the 16S ribosomal DNA for bacteria and the internal transcribed spacer 1 gene for fungi, the study was performed using marker gene amplification coupled with Illumina HiSeq high‐throughput sequencing technologies. Correlation analysis and a distance‐based redundancy analysis were performed to determine the dominant factors affecting the distribution of the microorganism in safflower soils. A total of 16517 bacterial operational taxonomic units (OTUs) were obtained from all the 108 soil samples of nine safflower sampling plots. At the phylum level, 48 phyla have been identified with Actinobacteria (32.9%) and proteobacteria (28.7%) being predominant. For fungi, 8746 OTUs were obtained, which belonged to seven phyla with Ascomycota overwhelmingly superior in relative abundance. A significant positive correlation was found between soil microbe quantity and ASL (above sea level). Safflower was sensitive to changes in elevation, growing more abundantly in the mountainous regions at heights of around 1,200 m above sea level. It is concluded that the dominant factors affecting the distribution of microorganisms in safflower soils were soil moisture, available N, and ASL.

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Section: Discussionmentioning

confidence: 99%

Study on the correlation between soil microbial diversity and ambient environmental factors influencing the safflower distribution in Xinjiang

et al. 2020

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“…Several aspects of microbial activity have been proposed as soil quality measures due to their sensitivity, and have been used as bioindicators of degradation processes (Ros et al, 2003(Ros et al, , 2009). Fungi are a fundamental component of soil microbial communities (Hagn et al, 2003;Orlandelli et al, 2012), and play important roles in many essential processes, including organic matter decomposition, elemental release by mineralization, and protection against leaching by elemental storage in biomass (Hagn et al, 2003;Malosso et al, 2006;Premalatha et al, 2015). Moreover, their mycelia contribute to soil aggregate stability, thereby preventing erosion (Rao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effect of karst rocky desertification on soil fungal communities in Southwest China

Wang¹,

Bt²,

Chen³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Karst mountainous ecosystems are associated with karst rocky desertification (KRD), which can greatly impact soil structure and function. Despite the importance of soil microbes as a major factor maintaining ecosystem stability, we know little about the effect on soil fungal communities of KRD in karst regions. We investigated this relationship across a gradient of KRD soils from Guizhou, China by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE). Fungal diversity indices (Shannon-Wiener, richness, and evenness) significantly differed (P < 0.05) based on KRD severity, being lowest in moderately affected areas. Cluster analysis showed that the five sites examined clustered into two main groups according to KRD grade (high and low). Moreover, a homology search using sequences recovered from PCR-DGGE bands showed that the dominant fungi in each community varied remarkably, and included Aspergillus, Aphanoascus, Blastomyces, Fusarium, Glomus, Geomyces, Gibberella, Mortierella, Tetracladium, and Tumularia species, and an unclassified group. In conclusion, these findings demonstrate that KRD has a significant impact on soil fungal communities.

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“…In addition, the populations were recorded to have restricted distribution and high fragmentation and were found to be over-exploited by destructive harvesting. Niche specificity, damage by fruit wasp, population bottleneck, restricted gene flow, and root rot by fungus are the various factors, which have endangered this group in their wild habitat 16 , 17 . This signals the need for taxon recognition in biodiversity hotspots, which is a key factor for the enforcement of plant protection regulations and the future conservation of the species 18 .…”

Section: Introductionmentioning

confidence: 99%

Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae)

Mishra

Kumar

Gokul

et al. 2017

Sci Rep

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The steno-endemic species of genus Decalepis are highly threatened by destructive wild harvesting. The medicinally important fleshy tuberous roots of Decalepis hamiltonii are traded as substitute, to meet the international market demand of Hemidesmus indicus. In addition, the tuberous roots of all three species of Decalepis possess similar exudates and texture, which challenges the ability of conventional techniques alone to perform accurate species authentication. This study was undertaken to generate DNA barcodes that could be utilized in monitoring and curtailing the illegal trade of these endangered species. The DNA barcode reference library was developed in BOLD database platform for candidate barcodes rbcL, matK, psbA-trnH, ITS and ITS2. The average intra-specific variations (0–0.27%) were less than the distance to nearest neighbour (0.4–11.67%) with matK and ITS. Anchoring the coding region rbcL in multigene tiered approach, the combination rbcL + matK + ITS yielded 100% species resolution, using the least number of loci combinations either with PAUP or BLOG methods to support a character-based approach. Species-specific SNP position (230 bp) in the matK region that is characteristic of D. hamiltonii could be used to design specific assays, enhancing its applicability for direct use in CITES enforcement for distinguishing it from H. indicus.

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Molecular profiling of fungal assemblages in the healthy and infected roots of Decalepis arayalpathra (J. Joseph & V. Chandras) Venter, an endemic and endangered ethnomedicinal plant from Western Ghats, India

Cited by 5 publications

References 23 publications

Study on the correlation between soil microbial diversity and ambient environmental factors influencing the safflower distribution in Xinjiang

Study on the correlation between soil microbial diversity and ambient environmental factors influencing the safflower distribution in Xinjiang

Effect of karst rocky desertification on soil fungal communities in Southwest China

Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae)

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