Molecular prophage typing of avian pathogenic Escherichia coli

Kwon, Hyuk‐Joon; Seong, Won-Jin; Kim, Jae Hong

doi:10.1016/j.vetmic.2012.10.005

Cited by 14 publications

(11 citation statements)

References 37 publications

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“…Salmonella enterica prophage sequence profiles can reflected genome diversity and can be used for high discrimination subtyping (Mottawea et al, 2018). Prophages have also been used for bacterial typing in E. coli , Streptococcus pneumoni a, and Bacillus anthracis based on their ubiquitous and unique features (Sozhamannan et al, 2006; Romero et al, 2009; Kwon et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Novel Yersinia enterocolitica Prophages and a Comparative Analysis of Genomic Diversity

et al. 2019

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Yersinia enterocolitica is a major agent of foodborne diseases worldwide. Prophage plays an important role in the genetic evolution of the bacterial genome. Little is known about the genetic information about prophages in the genome of Y. enterocolitica , and no pathogenic Y. enterocolitica prophages have been described. In this study, we induced and described the genomes of six prophages from pathogenic Y. enterocolitica for the first time. Phylogenetic analysis based on whole genome sequencing revealed that these novel Yersinia phages are genetically distinct from the previously reported phages, showing considerable genetic diversity. Interestingly, the prophages induced from O:3 and O:9 Y. enterocolitica showed different genomic sequences and morphology but highly conserved among the same serotype strains, which classified into two diverse clusters. The three long-tailed Myoviridae prophages induced from serotype O:3 Y. enterocolitica were highly conserved, shared ≥99.99% identity and forming genotypic cluster A; the three Podoviridae prophages induced from the serotype O:9 strains formed cluster B, also shared more than 99.90% identity with one another. Cluster A was most closely related to O:5 non-pathogenic Y. enterocolitica prophage PY54 (61.72% identity). The genetic polymorphism of these two kinds of prophages and highly conserved among the same serotype strains, suggested a possible shared evolutionary past for these phages: originated from distinct ancestors, and entered pathogenic Y. enterocolitica as extrachromosomal genetic components during evolution when facing selective pressure. These results are critically important for further understanding of phage roles in host physiology and the pathology of disease.

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Section: Discussionmentioning

confidence: 99%

Novel Yersinia enterocolitica Prophages and a Comparative Analysis of Genomic Diversity

et al. 2019

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“…The terminase large subunit gene was used to identify prophages in bacterial genomes and for the differentiation of phages from environments and uncultured bacteria [ 3 4 35 ]. A molecular prophage typing (mPPTing) method based on the terminase large subunit gene has been developed for molecular epidemiological study of avian pathogenic Escherichia coli in chickens and has provided additional information regarding phage-carrying virulence genes [ 24 ]. In addition, mPPTing with integrase genes in temperate phages of S. aureus has been developed [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular prophage typing ofStaphylococcus aureusisolates from bovine mastitis

Seong

Kim

et al. 2018

J Vet Sci

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Staphylococcus aureus is one of the major pathogens causing bovine mastitis and foodborne diseases associated with dairy products. To determine the genetic relationships between human and bovine or bovine isolates of S. aureus, various molecular methods have been used. Previously we developed an rpoB sequence typing (RSTing) method for molecular differentiation of S. aureus isolates and identification of RpoB-related antibiotic resistance. In this study, we performed spa typing and RSTing with 84 isolates from mastitic cows (22 farms, 72 cows, and 84 udders) and developed a molecular prophage typing (mPPTing) method for molecular epidemiological analysis of bovine mastitis. To compare the results, human isolates from patients (n = 14) and GenBank (n = 166) were used for real and in silico RSTing and mPPTing, respectively. Based on the results, RST10-2 and RST4-1 were the most common rpoB sequence types (RSTs) in cows and humans, respectively, and most isolates from cows and humans clearly differed. Antibiotic resistance-related RSTs were not detected in the cow isolates. A single dominant prophage type and gradual evolution through prophage acquisition were apparent in most of the tested farms. Thus, RSTing and mPPTing are informative, simple, and economic methods for molecular epidemiological analysis of S. aureus infections.

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“…Cyanobacterial cells differentiate into nitrogen-fixing cells along with the non-reversible excision of prophage elements from three nitrogen fixation process-related genes under nitrogen-starving conditions [ 12 ]. Based on their ubiquitous and unique features, prophages have been used for bacterial typing in Escherichia coli , Streptococcus pneumonia , and Bacillus anthracis [ 16 – 18 ]. However, despite a number of B .…”

Section: Introductionmentioning

confidence: 99%

Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus

et al. 2017

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Cereulide-producing Bacillus cereus isolates can cause serious emetic (vomiting) syndrome and even acute lethality. As mobile genetic elements, the exploration of prophages derived from emetic B. cereus isolates will help in our understanding of the genetic diversity and evolution of these pathogens. In this study, five temperate phages derived from cereulide-producing B. cereus strains were induced, with four of them undergoing genomic sequencing. Sequencing revealed that they all belong to the Siphoviridae family, but presented in different forms in their hosts. PfNC7401 and PfIS075 have typical icosahedral heads, probably existing alone as phagemids in the host with self-replicating capability in the lysogenic state. PfEFR-4, PfEFR-5, and PfATCC7953 have elongated heads, with the genomes of the former two identified as linear dsDNA, which could be integrated into the host genome during the lysogenic state. Genomic comparison of the four phages with others also derived from emetic B. cereus isolates showed similar genome structures and core genes, thus displaying host spectrum specificity. In addition, phylogenic analysis based on the complete genome and conserved tail fiber proteins of 36 Bacillus species-derived phages confirmed that the phages derived from emetic B. cereus strains were highly similar. Furthermore, one endolysin LysPfEFR-4 was cloned and showed lytic activity against all tested emetic B. cereus strains and cross-lytic activity against some other pathogenic bacteria, implying a potential to control bacterial contamination in the food supply.

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Molecular prophage typing of avian pathogenic Escherichia coli

Cited by 14 publications

References 37 publications

Novel Yersinia enterocolitica Prophages and a Comparative Analysis of Genomic Diversity

Novel Yersinia enterocolitica Prophages and a Comparative Analysis of Genomic Diversity

Molecular prophage typing ofStaphylococcus aureusisolates from bovine mastitis

Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus

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