Molecular quantification of sulfate-reducing microorganisms (carrying dsrAB genes) by competitive PCR in estuarine sediments

Leloup, Julie; Quillet, Laurent; Oger, CÃ©cile; Boust, D.; Petit, Fabienne

doi:10.1016/s0168-6496(03)00262-9

Cited by 38 publications

(36 citation statements)

References 47 publications

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“…Here, we proposed that Clostridium sp. BXM is classified as a novel non-dsrAB (without dissimilatory sulfite reductase genes) sulfate-reducing bacterium (Leloup et al, 2004). The present results also imply that Clostridium sp.…”

Section: Description Of Clostridium Sp Bxm and Environmental Implicasupporting

confidence: 68%

Dechlorination of p,p′-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp. BXM

Bao

Wang

et al. 2012

Environmental Pollution

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a b s t r a c tA novel non-dsrAB (without dissimilatory sulfite reductase genes) sulfate-reducing bacterium (SRB) Clostridium sp. BXM was isolated from a paddy soil. Incubation experiments were then performed to investigate the formation of reduced sulfur compounds (RSC) by Clostridium sp. BXM, and RSC-induced dechlorination of p,p 0 -DDT in culture medium and soil solution. The RSCs produced were 5.8 mM and 4.5 mM in 28 mM sulfate amended medium and soil solution respectively after 28-day cultivation. The p,p 0 -DDT dechlorination ratios were 74% and 45.8% for 5.8 mM and 4.5 mM RSCs respectively at 6 h. The metabolites of p,p 0 -DDT found in the two reaction systems were identified as p,p 0 -DDD and p,p 0 -DDE. The dechlorination pathways of p,p 0 -DDT to p,p 0 -DDD and p,p 0 -DDE were proposed, based on mass balance and dechlorination time-courses. The results indicated that RSC-induced natural dechlorination may play an important role in the fate of organochlorines.Crown

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Section: Description Of Clostridium Sp Bxm and Environmental Implicasupporting

confidence: 68%

Dechlorination of p,p′-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp. BXM

Bao

Wang

et al. 2012

Environmental Pollution

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“…These sequences were phylogenetically distant from any of the isolated SRB lineages but were related to dsrA clones recovered from uncultured SRB in environmental samples including one from uranium mill tailing groundwater (Chang et al 2001), an acidic fen (Loy et al 2004), Lake Fryxell (Karr et al 2005), Guaymas Basin (Dhillon et al 2003) and the Seine estuary (Leloup et al 2004(Leloup et al , 2006.…”

Section: Diversity Of Srb Based On Dsramentioning

confidence: 94%

Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Kondo¹,

Osawa²,

Mochizuki³

et al. 2006

Plankton Benthos Res

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Abstract:The depth distribution of sulphate-reducing bacteria (SRB) in the water column of a meromictic lake, Lake Suigetsu, Fukui, Japan was investigated using quantitative competitive PCR targeting the gene coding for portions of the a-subunit of dissimilatory sulphite reductase (dsrA). The total bacterial cell density (DAPI count) was 5Ϫ13ϫ10 6 cells mL Ϫ1 in the water column of the lake with maximum abundance occurring at the oxic-anoxic interface layer. SRBwere not detected in oxic surface water using competitive PCR. SRB were found in the anoxic waters below the oxycline ranging from 10 4 to 10 5 cells mL Ϫ1 , accounting for 0.3-8.9% of the total bacteria. The SRB cell densities were higher than previously estimated using the most-probable-number (MPN) method. Sequencing of the cloned PCR product of dsrA showed the existence of different SRB groups in the anoxic water. The majority of the dsrA sequences were associated with the Desulfosarcina-Desulfococcus-Desulfonema group and members of the Desulfobulbaceae family.Other dsrA clones belonged to the Desulfomicrobium and Desulfovibrio species as well as to a deeply branched group in the dsrA tree with no representatives from previously isolated SRB groups. These SRB species appear to be important for the sulphur and carbon cycle in the anoxic waters of Lake Suigetsu.

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“…OTU-47 to -87 formed a monophyletic group without any isolated SRB lineages. However, clone sequences in these OTUs were related to dsrA clones recovered from uncultured SRB in marine and freshwater environmental samples (see Table 2) (Dhillon et al 2003;Leloup et al 2004;Loy et al 2004;Mußmann et al 2005). Clones in OTU-88 to -92 also formed a monophyletic group.…”

Section: Julymentioning

confidence: 99%

Comparison of the diversity of sulfate-reducing bacterial communities in the water column and the surface sediments of a Japanese meromictic lake

Kondo

Butani

2007

Limnology

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Comparison of the diversity of sulfate-reducing bacterial communities in the water column and the surface sediments of a Japanese meromictic lake Abstract The diversity and abundance of sulfate-reducing bacteria (SRB) were investigated in Lake Suigetsu, a meromictic lake in Japan characterized by a permanent oxycline at a depth between 3 and 8 m separating the aerobic freshwater epilimnion from the anaerobic, saline, sulfi dogenic hypolimnion. A quantitative competitive PCR targeting the gene coding for a portion of the α-subunit of dissimilatory sulfi te reductase (dsrA) was used to assess the distribution of the SRB in the stratifi ed water column and the surface sediments. The diversity of the SRB communities was assessed using a sequence analysis of the differing dsrA isomers. The dsrA gene copy numbers of SRB in the hypolimnic waters were from 9.6 × 10 3 to 7.5 × 10 5 copies ml −1 , whereas higher dsrA copy numbers of SRB were observed in surface sediments, ranging from 1.8-8.1 × 10 7 copies ml −1 as estimated by competitive PCR. Phylogenetic analysis of the dsrA sequences retrieved from the surface sediments shows most belong to a deeply branching lineage of diverse dsrA sequences not related to any cultured SRB group. In contrast, dsrA sequences found in the oxycline waters were related to sequences of members of the genera Desulfonema, Desulfosarcina, and Dusulfococcus and to sequences of the incomplete oxidizers from the Desulfobulbaceae family. Diversity and abundance of dsrA genes signifi cantly differed between the samples from the oxycline waters and the surface sediments of Lake Suigetsu, indicating habitat-specifi c SRB communities may contribute to the biogeochemical cycling of carbon and sulfur.

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Molecular quantification of sulfate-reducing microorganisms (carrying dsrAB genes) by competitive PCR in estuarine sediments

Cited by 38 publications

References 47 publications

Dechlorination of p,p′-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp. BXM

Dechlorination of p,p′-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp. BXM

Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Comparison of the diversity of sulfate-reducing bacterial communities in the water column and the surface sediments of a Japanese meromictic lake

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