Molecular responses of Nile tilapia ( Oreochromis niloticus ) to different levels of dietary carbohydrates

Boonanuntanasarn, Surintorn; Kumkhong, Suksan; Yoohat, Kirana; Plagnes-Juan, Élisabeth; Burel, Christine; Marandel, Lucie; Panserat, Stéphane

doi:10.1016/j.aquaculture.2017.09.032

Cited by 60 publications

(42 citation statements)

References 38 publications

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“…These results confirmed that fish ate well during the experiment. Interestingly, blood glucose levels of fasted fish were surprisingly low compared to data obtained in other teleosts (carnivorous trout [15] or omnivorous tilapia [16,17], zebrafish [18] or goldfish [19]) but were in accordance with results previously published by Riddle et al [6] and measured with the same glucometer reference.…”

Section: Fish Ate Well During the Nutritional Challengesupporting

confidence: 90%

Nutritional regulation of glucose metabolism-related genes in the emerging teleost model Mexican tetra surface fish: a first exploration

et al. 2020

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Astyanax mexicanus has gained importance as a laboratory model organism for evolutionary biology. However, little is known about its intermediary metabolism, and feeding regimes remain variable between laboratories holding this species. We thus aimed to evaluate the intermediary metabolism response to nutritional status and to low (NC) or high (HC) carbohydrate diets in various organs of the surface-dwelling form of the species. As expected, glycaemia increased after feeding. Fish fed the HC diet had higher glycaemia than fish fed the NC diet, but without displaying hyperglycaemia, suggesting that carbohydrates are efficiently used as an energy source. At molecular level, only fasn ( Fatty Acid Synthase ) transcripts increased in tissues after refeeding, suggesting an activation of lipogenesis. On the other hand, we monitored only moderate changes in glucose-related transcripts. Most changes observed were related to the nutritional status, but not to the NC versus HC diet. Such a metabolic pattern is suggestive of an omnivorous-related metabolism, and this species, at least at adult stage, may adapt to a fish meal-substituted diet with high carbohydrate content and low protein supply. Investigation to identify molecular actors explaining the efficient use of such a diet should be pursued to deepen our knowledge on this species.

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Section: Fish Ate Well During the Nutritional Challengesupporting

confidence: 90%

Nutritional regulation of glucose metabolism-related genes in the emerging teleost model Mexican tetra surface fish: a first exploration

et al. 2020

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“…In the C120 group, down‐regulation of pck1 and g6pc mRNA levels in the liver of Japanese flounder could be a result of reduced gluconeogenesis, avoiding hyperglycaemia and lower metabolite requirements from non‐carbohydrates such as proteins and lipids. In this study, no significant effects on plasma glucose levels were observed in the C120 group, suggesting the regulation of that metabolic pathway resulted in better glucose storage and improved glucose homoeostasis (Boonanuntanasarn, Kumkhong, et al, ).…”

Section: Discussionmentioning

confidence: 57%

Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

et al. 2019

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Japanese flounder juveniles (initial body weight: 7.12 ± 0.02 g) were fed three diets containing 0, 120 and 200 g/kg of carbohydrates (C0, C120 and C200, respectively) for 10 weeks. Results showed that higher dietary carbohydrate intake enabled further deposition of glycogen and lipids in liver and muscle. The mRNA levels of glucokinase (gck), phosphofructokinase (pfkl) and hexokinase (HK) activity involved in glycolysis were significantly up‐regulated in C120 (p < .05) but showed no further up‐regulation except for gck in C200. Besides, the gluconeogenic phosphoenolpyruvate carboxykinase 1 (pck1) mRNA levels were down‐regulated significantly in fish fed the C120 (p < .05). However, further increase in dietary carbohydrate levels increased the mRNA levels and activities of enzymes involved in gluconeogenesis and lipolysis (p < .05). Additionally, plasma glucose remained unchanged in C120 (p > .05) but significantly increased in C200 group (p < .05). In conclusion, Japanese flounder was able to use carbohydrates efficiently through regulation of glucose and lipid metabolism when dietary carbohydrate was not higher than 120 g/kg, while 200 g/kg of dietary carbohydrate caused the deregulation of glucose homoeostasis.

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“…This fact explains the occurrence of glycogenesis, by the availability of blood glucose by the carbohydrate source (MPM), leading to the synthesis of glycogen in the liver from glucose. The Nile tilapia can use high carbohydrate levels without any deregulation of glucose homeostasis (Boonanuntanasarn et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

INCLUSION OF MESQUITE POD MEAL (<i>Prosopis juliflora</i>) IN DIETS FOR NILE TILAPIA (<i>Oreochromis niloticus</i>) JUVENILES

et al. 2019

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Alternative feeds are used to include or replace conventional ingredients in fish diets to generate regional socioeconomic sustainability and maintain the growth performance of fish. The objective of this study was to evaluate the effect of mesquite pod meal (MPM) in extruded diets for Nile tilapia juveniles fed graded levels of inclusion (26, 32, 38, 44, and 50%). Growth performance variables of plasma metabolites, enzymatic activities and haematological parameters were evaluated. Fish were fed at 6% of biomass for 45 days. Four-hundred Nile tilapias juveniles (11.5 ± 0.18 g) were distributed into twenty-500L aquaria in recirculation system. Significant differences of treatments on performance parameters, feed conversion ratio and survival were observed. Among the metabolic parameters, glycemia was altered (P <0.05). The activities of digestive and transamination enzymes were not influenced (P>0.05) by the inclusion of MPM. Hematological variables did not change (P>0.05). It was concluded that MPM can be included up to 38.67% in diets for Nile tilapia juveniles without impairing performance, metabolism, enzymatic activities and health.

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Molecular responses of Nile tilapia ( Oreochromis niloticus ) to different levels of dietary carbohydrates

Cited by 60 publications

References 38 publications

Nutritional regulation of glucose metabolism-related genes in the emerging teleost model Mexican tetra surface fish: a first exploration

Nutritional regulation of glucose metabolism-related genes in the emerging teleost model Mexican tetra surface fish: a first exploration

Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

INCLUSION OF MESQUITE POD MEAL (<i>Prosopis juliflora</i>) IN DIETS FOR NILE TILAPIA (<i>Oreochromis niloticus</i>) JUVENILES

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