1997
DOI: 10.1104/pp.115.2.593
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Molecular Rigidity in Dry and Hydrated Onion Cell Walls

Abstract: Solid-state nuclear magnetic resonance relaxation experiments can provide information on the rigidity of individual molecules within a complex structure such as a cell wall, and thus show how each polymer can potentially contribute to the rigidity of the whole structure. We measured the proton magnetic relaxation parameters T2 (spin-spin) and T,p (spin-lattice) through the 13C-nuclear magnetic resonance spectra of dry and hydrated cell walls from onion (Allium cepa L.) bulbs. Dry cell walls behaved as rigid so… Show more

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Cited by 113 publications
(100 citation statements)
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“…It also rules out the possibility of studying the effects of controlled mechanical stress on the sample, and normally restricts the sample size to about 0.1 g so that some thousands of repetitions of each spectral measurement must be averaged to obtain an adequate signal/noise ratio. This can lead to very long durations for 13 C NMR experiments. These can be reduced by …”
Section: Solid-state Nmrmentioning
confidence: 99%
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“…It also rules out the possibility of studying the effects of controlled mechanical stress on the sample, and normally restricts the sample size to about 0.1 g so that some thousands of repetitions of each spectral measurement must be averaged to obtain an adequate signal/noise ratio. This can lead to very long durations for 13 C NMR experiments. These can be reduced by …”
Section: Solid-state Nmrmentioning
confidence: 99%
“…It does this through the tiny magnetic fields created at the nucleus by orbiting electrons. Cell-wall polysaccharides in solution give detailed and highly informative 13 C and 1 H NMR spectra. However, if intact cell walls are examined in a conventional, solution-state NMR spectrometer, the 13 C or proton spectrum is so broad that it cannot be seen.…”
Section: Solid-state Nmrmentioning
confidence: 99%
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“…Classical models of the primary cell wall in land plants describe the wall as a composite structure composed of load-bearing, semicrystalline cellulose fibrils surrounded by an amorphous matrix of cross-linking glycans (hemicelluloses and pectins), structural (glyco)proteins, and, in some cases, polyphenolics (Carpita and Gibeaut, 1993;Carpita and McCann, 2000;Cosgrove, 2005;Jarvis, 2009). Moreover, primary plant cell walls have a high water content (approximately 80% in primary walls; Jarvis, 2009), which is responsible for maintaining the structure in a dynamic, hydrogel-like state (Ha et al, 1997;Zwieniecki et al, 2001;Jarvis, 2009). Contemporary structural analysis, using increasingly sophisticated probes, is refining our understanding of the exquisite spatial localization of cell wall polymers (Knox, 2008;Yarbrough et al, 2009).…”
mentioning
confidence: 99%