Molecular Screening of Staphylococcal Enterotoxin Type P Encoding Gene From Clinical Isolates

Ataee, Ramezan Ali; Hedaiatich, Mojtaba; Khanshan, Rahim Mansuor; Ataee, Mohammad Hosein

doi:10.5812/jjm.6365

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…For example, the sed and sej genes are located at the same plasmids; so, it was not surprising we could not detect both genes. At the moment, the enterotoxin producing strains are spread extensively in the world and have become a challenge for public health and involved in colonization and a broad spectrum diseases (37,38).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Virulence Factors in Methicillin-Resistant Staphylococcus aureus Strains Isolated From a Referral Hospital in Tehran, Iran

Rahimi

Karimi

2016

Arch Clin Infect Dis

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) has been known as one of the most important nosocomial pathogens that able to produce a variety of virulence factors. Objectives: In this study, we aimed to describe the prevalence, presence of different virulence factors, staphylococcal cassette chromosome mec (SCCmec) and prophage typing of MRSA strains isolated from a referral hospital in Tehran, Iran. Materials and Methods: A total of 279 S. aureus strains were collected from a referral hospital in Tehran during August to December of 2013. All isolates were confirmed using species specific primers and were tested for susceptibility to oxacillin and cefoxitin disks by the recommendations of clinical and laboratory standards institute (CLSI). The staphylococcal enterotoxin (sea-seq) and pvl, hlb and sak genes were detected and typed using prophage typing and SCCmec typing methods. Results: Out of the 279 S. aureus isolates, 91 (32.6%) strains were confirmed as MRSA. Totally, 6 enterotoxin and 2 virulence factor genes were detected in MRSA strains. The sea, sek, seq and hlb genes were present in all MRSA and sak, seg, sei and sel were detected in 85%, 35%, 23% and 44% of the strains. Only SCCmec type III and type 3 ccr and 2 different prophage patterns were identified among the strains. Conclusions: Our results show the presence of clonal groups of enterotoxin-producing MRSA strains in this hospital in Tehran. The presence of bacteriophage encoded virulence factors and resistance to oxacillin enable bacteria to produce a broad spectrum range of diseases.

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Section: Discussionmentioning

confidence: 99%

Characteristics of Virulence Factors in Methicillin-Resistant Staphylococcus aureus Strains Isolated From a Referral Hospital in Tehran, Iran

Rahimi

Karimi

2016

Arch Clin Infect Dis

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“…The EBV viral particles may act as potent superantigens that are involved in triggering inflammatory diseases, such as RA. It should be noted that this is not the only superantigen that contributes to RA disease, since recent research has indicated that several other bacterial superantigens, including Staphylococcal ( 30 – 32 ) and Mycoplasma superantigens ( 33 , 34 ), were reported in the synovial fluids of RA patients. In addition, the existence of Staphylococcal superantigens in CSF of meningitides patients were reported ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

Detection of Epstein-Barr virus in synovial fluid of rheumatoid arthritis patients

Mahabadi¹,

Faghihiloo²,

Alishiri³

et al. 2016

Electron physician

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IntroductionRheumatoid arthritis (RA) is one of the most common chronic inflammatory disorders. Genes and environmental factors contribute to RA. Epstein–Barr Virus (EBV) has been considered as one the RA pathogeneses. The aim of this study was to detect of the EBV genome in patients with RA.MethodsIn this cross-sectional study, 50 samples of synovial fluid were obtained from patients with RA from 2010–2012. Using a standard of the EBV genome and EBNA-1-specific primers, the method of PCR was set up. Then, all of the samples of synovial fluids separately were subjected to DNA extraction and Polymerase Chain Reaction (PCR) amplification. Data were analyzed using SPSS version 18.0. The statistical analysis was performed by the t-test.ResultsThe demographic and laboratory characteristic assay revealed that the mean age of patients was 49, and the patients were 60% males and 40% females. In addition, in all cases, the mean rheumatoid factor (RF) levels of the patients were below the normal level. The results of this study showed that the PCR was able to detect EBV DNA in > 60% of the cases.ConclusionThe results of this study indicated that EBV was frequently detected in the synovial fluid of RA patients. Thus, EBV may be a strong candidate that can act at several levels of the pathophysiology of RA. However, these findings also indicated that EBV may play a role in the pathogenesis of RA. However, the possible relationship between RA and EBV must be determined by further research.

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“…Regulated by the staphylococcal two-component system, accessory gene regulatory (agr), the region between 59 and 93 nucleotides upstream of the transcription ignition site was found to be essential for transcription and expression [92][93][94]. seb had been commonly found in toxin-mediated foodborne and clinical S. aureus strains, and recently, seb (by PCR and Western blotting) was detected from 5% of 300 clinical Staphylococcus strains [95].…”

Section: Staphylococcal Enterotoxin B (Seb and Seb)mentioning

confidence: 99%

Staphylococcal Food Poisoning and Novel Perspectives in Food Safety

Xu¹,

Peters²,

Li³

et al. 2016

Significance, Prevention and Control of Food Related Diseases

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Ingestion of food is the major way for human beings to obtain nutrient substances for basic living, and therefore, the quality and safety of food is a major concern. Foodborne illness includes any illness resulting from the consumption of contaminated food that contains pathogenic bacteria, viruses, or chemical or natural toxins. Consequently, food safety is considered to be a globally expanding issue and thus a leading topic in public health, no longer limited to foodborne illnesses but extended to nearly every safety issue regarding "farm to table" food approaches. Bacterial foodborne infections occur by ingestion of food contaminated with growth of pathogenic bacteria, toxin production, and continuous bacterial growth in intestines. In the past decade, a large number of cases or reports have been available on food containing unhealthy, harmful, or toxic substances (other than food poisoning outbreaks) worldwide. Foodborne microorganisms, primarily associated with pathogenic bacteria and toxic substances produced in food, have presented major challenges for food safety. As a global foodborne pathogen, Staphylococcus aureus is typically capable of causing a large number of infections, including food poisoning. Thus, this chapter aims to review several factors contributing to the rise of staphylococci as a growing concern for the food safety industry, including the growth of S. aureus, production and regulation of staphylococcal enterotoxins, the viable putative but nonculturable (VPNC) state, and antimicrobial resistance of Staphylococcus caused by the indiscriminate use of antibiotics in both clinical and veterinary settings.

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Molecular Screening of Staphylococcal Enterotoxin Type P Encoding Gene From Clinical Isolates

Cited by 4 publications

References 39 publications

Characteristics of Virulence Factors in Methicillin-Resistant Staphylococcus aureus Strains Isolated From a Referral Hospital in Tehran, Iran

Characteristics of Virulence Factors in Methicillin-Resistant Staphylococcus aureus Strains Isolated From a Referral Hospital in Tehran, Iran

Detection of Epstein-Barr virus in synovial fluid of rheumatoid arthritis patients

Staphylococcal Food Poisoning and Novel Perspectives in Food Safety

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