Molecular signatures of in vitro produced embryos derived from ovum pick up or slaughterhouse oocytes in buffalo

Yang, Chunyan; Zheng, Hongzhi; Abdelnour, Sameh A.; Li, Ling-yu; Shokrollahi, Borhan; Tang, Lixuan; Zhang, Yu; Huang, Jia‐Xiang; Shang, Jin

doi:10.1016/j.theriogenology.2021.03.025

Cited by 6 publications

(6 citation statements)

References 36 publications

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“…Oocytes are mostly towards cortical portion with few primordial follicles towards the cortico medullary region. The same arrangement of oocytes is observed in the premature mice (CY Yang, et al [33]). Primordial follicles in Nili-Ravi buffalo fetal ovary are from 118-288 days of gestation.…”

Section: Discussionsupporting

confidence: 62%

“…Dangudubiyyam [31] reported that the weight of the right ovary (RO) was greater than left ovary (LO) (P < 0.05) with 0.393 ± 0.04 g for RO than LO with 0.355 ± 0.05 g. In Nili-Ravi buffalo ovaries in the 3 rd month of fetal age (30.08 ± 2.28 mg) and 9 th month of fetal age (163.0 ± 39.00) are heavier compared to Egyptian buffalo in the same fetal ages (3 rd months -27.0 ± 4.3 mg; 9 th month 94.7 ± 39.0 mg) (Alkafafy,et al [15,32,33]). In Nili-Ravi buffalo fetus length (P ≤ 0.001) and width (P ≤ 0.001) of right and left ovaries increase significantly with the increase in age.…”

Section: Discussionmentioning

confidence: 99%

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"Appraisal to Prenatal Ovarian Traits in Morph-Metric Aspects of Nili-Ravi Buffalo Bovine Fetus"

Ullah

2023

BJSTR

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This study aimed to evaluate the Nili-Ravi buffalo changes in fetal growth relationship with crown rump length (C-R) ovarian at fetal ages from 51 to 290 days. In the methods, the weight, size, and other proximate measurements were compared with the ovarian histology of female sex were externally differentiated up to 56 days. In the results, a linear trend in increase between C-R length, age and body weight is observed. The mean weight of prenatal ovary from 51-70-day-old fetus was 18.8 ± 2.33 mg which increased to 163.30±39.00 mg at 271-290 days of fetal age. Linear regression showed highly significant increase with age in right ovarian weight (b=4.89, P <0.001), and left ovarian weight (b=4.79, P <0.001). In conclusion, the novelty of the distinguished age for differentiating the fetus ovary could have critical information of altered ovarian steroidogenesis in a way that might affect sexual maturation in Nili-Ravi Buffalo.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

"Appraisal to Prenatal Ovarian Traits in Morph-Metric Aspects of Nili-Ravi Buffalo Bovine Fetus"

Ullah

2023

BJSTR

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“…Despite being well established in some parts of the world, water buffaloes display an exceptional set of challenges owing to their late sexual maturity, long generation intervals, and silent heat, which impedes genetic improvement [ 1 , 2 ]. For enhancing the reproductive capacity of water buffaloes, assisted reproductive technologies (ARTs) such as in vitro maturation (IVM), artificial insemination (AI), in vitro fertilization (IVF), cloning, and nuclear transfer have been utilized worldwide [ 1 , 3 , 4 ]. Semen cryopreservation is a crucial procedure for ART [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Khalil,

Hassan,

El-Harairy

et al. 2023

Animals

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Thymoquinone nanoparticles (TQNPs) are broadly utilized in numerous pharmaceutical applications. In the present study, we tested the effects of TQNP supplementation on sperm quality and kinematics, acrosome exocytosis, oxidative biomarkers, apoptosis-like and morphological changes of frozen–thawed buffalo sperm, as well as the fertilizing capacity. Semen was collected from buffalo bulls, diluted (1:10; semen/extender), and divided into five aliquots comprising various concentrations of TQNP 0 (CON), 12.5 (TQNP12.5), 25 (TQNP25), 37.5 (TQNP37.5), and 50 (TQNP50) µg/mL, and then cryopreserved and stored in liquid nitrogen (−196 °C). The results revealed that TQNPs (25 to 50 µg/mL) provided the most optimal results in terms of membrane integrity (p < 0.001) and progressive motility (p < 0.01). In contrast, TQNP50 resulted in a greater post-thawed sperm viability (p = 0.02) compared with other groups. The addition of TQNPs to the extender had no discernible effects on sperm morphology measures. Sperm kinematic motion was significantly improved in the TQNP50 group compared to the control group (p < 0.01). TQNPs effectively reduced the content of H2O2 and MDA levels and improved the total antioxidant capacity of post-thawed extended semen (p < 0.01). The addition of TQNP significantly increased the number of intact acrosomes (p < 0.0001) and decreased the number of exocytosed acrosomes (p < 0.0001). A significant reduction in apoptosis-like changes was observed in TQNP groups. The non-return rates of buffalo cows inseminated with TQNP50-treated spermatozoa were higher than those in the control group (p < 0.05; 88% vs. 72%). These findings suggested that the freezing extender supplemented with TQNPs could effectively enhance the cryotolerance and fertility of buffalo sperm.

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“…Buffalo is an important livestock resource in many Asian and Mediterranean countries [1,2]. In vitro embryo production (IVEP) efficiency in buffalo is lower than in cattle, according to research, and this could be improved further by selecting highquality oocytes and/or improving the in vitro embryo culture system [3] [4] [5] [6]. The lack of synchronization between nuclear and cytoplasmic maturation, as well as insufficient cytoplasmic maturation, results in oocytes matured in vitro having lower developmental competence than those matured in vivo [3,4,6,7].…”

Section: Introductionmentioning

confidence: 99%

“…In vitro embryo production (IVEP) efficiency in buffalo is lower than in cattle, according to research, and this could be improved further by selecting highquality oocytes and/or improving the in vitro embryo culture system [3] [4] [5] [6]. The lack of synchronization between nuclear and cytoplasmic maturation, as well as insufficient cytoplasmic maturation, results in oocytes matured in vitro having lower developmental competence than those matured in vivo [3,4,6,7]. Despite extensive research into factors affecting oocyte maturation in mice and other animals, the molecular mechanism governing oocyte maturation in buffalo remains unknown [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Comparing in vitro maturation rates in buffalo and cattle oocytes and evaluating the effect of cAMP modulators on maturation and subsequent developmental competence

Abdulkarim

Balboula

Badr³

et al. 2021

Mansoura Veterinary Medical Journal

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Molecular signatures of in vitro produced embryos derived from ovum pick up or slaughterhouse oocytes in buffalo

Cited by 6 publications

References 36 publications

"Appraisal to Prenatal Ovarian Traits in Morph-Metric Aspects of Nili-Ravi Buffalo Bovine Fetus"

"Appraisal to Prenatal Ovarian Traits in Morph-Metric Aspects of Nili-Ravi Buffalo Bovine Fetus"

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Comparing in vitro maturation rates in buffalo and cattle oocytes and evaluating the effect of cAMP modulators on maturation and subsequent developmental competence

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