Molecular strain typing of multidrug-resistant Klebsiella pneumoniae: capsular wzi gene sequencing versus multiple locus variable number tandem repeat analysis

Aboulela, Aliaa; Elsherbini, Eglal; Abu-Sheasha, Ghada Ahmed; El-Raouf, Hala Abd; El-Ghazzawi, E.; Gaballah, Ahmed

doi:10.1016/j.diagmicrobio.2020.115139

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Other conventional typing techniques have several limitations such as typeability, inadequate reproducibility, and insufficient discriminatory power [10] . To circumvent these limitations, more typing methods that use molecular techniques are being employed [31] . Using a digoxigenin-labeled rDNA probe and EcoRI RE, a ribotyping database of K. pneumoniae was created without the harmful effects of radioactive probes [28] .…”

Section: Molecular Typing Of K Pneumoniaementioning

confidence: 99%

Molecular typing methods & resistance mechanisms of MDR Klebsiella pneumoniae

2023

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<abstract> The emergence and transmission of carbapenem-resistant <italic>Klebsiella pneumoniae</italic> (CRKP) have been recognized as a major public health concern. Here, we investigated the molecular epidemiology and its correlation with the mechanisms of resistance in CRKP isolates by compiling studies on the molecular epidemiology of CRKP strains worldwide. CRKP is increasing worldwide, with poorly characterized epidemiology in many parts of the world. Biofilm formation, high efflux pump gene expression, elevated rates of resistance, and the presence of different virulence factors in various clones of <italic>K. pneumoniae</italic> strains are important health concerns in clinical settings. A wide range of techniques has been implemented to study the global epidemiology of CRKP, such as conjugation assays, 16S-23S rDNA, string tests, capsular genotyping, multilocus sequence typing, whole-genome sequencing-based surveys, sequence-based PCR, and pulsed-field gel electrophoresis. There is an urgent need to conduct global epidemiological studies on multidrug-resistant infections of <italic>K. pneumoniae</italic> across all healthcare institutions worldwide to develop infection prevention and control strategies. In this review, we discuss different typing methods and resistance mechanisms to explore the epidemiology of <italic>K. pneumoniae</italic> pertaining to human infections. </abstract>

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Section: Molecular Typing Of K Pneumoniaementioning

confidence: 99%

Molecular typing methods & resistance mechanisms of MDR Klebsiella pneumoniae

2023

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“…DNA was extracted from CRKP strains. Subsequently, capsular serotype-specific gene (wzi), virulence genes (e.g., fimH, mrkD, peg-344, rmpA, rmpA2 and icuA) and carbapenemase genes were amplified by PCR using specific primers as previously described (1,(18)(19)(20) (Table 1). The positive PCR products were verified by sequencing at Beijing Genomics Institute Technology Co. Ltd. (Shanghai, China).…”

Section: Polymerase Chain Reaction (Pcr) For Capsular Serotypes Virulence Genes and Carbapenemase Genesmentioning

confidence: 99%

Comparison of Carbapenem-Resistant Klebsiella pneumoniae Strains Causing Intestinal Colonization and Extraintestinal Infections: Clinical, Virulence, and Molecular Epidemiological Characteristics

Liao

Huang

Zhang

et al. 2021

Front. Public Health

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Carbapenem-resistant Klebsiella pneumonia (CRKP) infections has become a concerning threat. However, knowledge regarding the characteristics of intestinal CRKP isolates is limited. This study aimed to investigate and compare the clinical, virulence and molecular epidemiological characteristics of intestinal colonization and extraintestinal infections CRKP strains. The clinical characteristics were investigated retrospectively. Polymerase chain reaction was used to investigate the capsular serotype, virulence genes and carbapenemase genes. Capsular polysaccharide quantification assay, serum resistance assay, biofilm formation assay, and infection model of Galleria mellonella larvae were performed to compare the virulence and pathogenicity. Besides, multilocus-sequence-typing (MLST) and pulsed-field-gel-electrophoresis (PFGE) were conducted to explore the homology of intestinal CRKP isolates. A total of 54 intestinal CRKP isolates were included. The main capsular serotypes were K14, K64, and K19. C-reactive protein and the proportion of ICU isolation of the infection group were significantly higher than that of the colonization group (P < 0.05). The carrier rates of various virulence genes of CRKP in the infection group were mostly higher than those in the colonization group, wherein the carrier rates of peg-344 and rmpA were significantly different (P < 0.05). There was no significant difference in capsular polysaccharides, antiserum ability, biofilm formation ability between the two group (P > 0.05), but the lethality of the infection group to Galleria mellonella was significantly higher than that of the colonization group (P < 0.05). The MLST categorized the 54 isolates into 13 different sequence types. PFGE revealed that homology among the 54 CRKP strains was <80%. This study suggested that the CRKP strains in the infection group had higher virulence than those in the colonization group. The development of CRKP isolates colonizing in the intestine should be addressed in future clinical surveillance.

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Molecular typing of clinical multidrug-resistant Klebsiella pneumoniae isolates

Kashefieh,

Zeighami,

Samadi Kafil

et al. 2024

Mol Biol Rep

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Molecular strain typing of multidrug-resistant Klebsiella pneumoniae: capsular wzi gene sequencing versus multiple locus variable number tandem repeat analysis

Cited by 4 publications

References 17 publications

Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

Comparison of Carbapenem-Resistant Klebsiella pneumoniae Strains Causing Intestinal Colonization and Extraintestinal Infections: Clinical, Virulence, and Molecular Epidemiological Characteristics

Molecular typing of clinical multidrug-resistant Klebsiella pneumoniae isolates

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Molecular strain typing of multidrug-resistant Klebsiella pneumoniae: capsular wzi gene sequencing versus multiple locus variable number tandem repeat analysis

Cited by 4 publications

References 17 publications

Molecular typing methods &amp; resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

Molecular typing methods &amp; resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

Comparison of Carbapenem-Resistant Klebsiella pneumoniae Strains Causing Intestinal Colonization and Extraintestinal Infections: Clinical, Virulence, and Molecular Epidemiological Characteristics

Molecular typing of clinical multidrug-resistant Klebsiella pneumoniae isolates

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Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>