Molecular, Structural, and Rheological Characterization of Camel Skin Gelatin Extracted Using Different Pretreatment Conditions

Abstract

Optimum conditions for high-quality gelatin recovery from camel skin and its molecular, structural, and rheological characterization were carried out in this study. Increased yield and gel strength were recorded, with an increase in camel skin pretreatment times of 6 to 42 h and 0.50 and 0.75 M-NaOH. Gelatin from skin pretreated with 0.75 and 0.5 M-NaOH for 42 h showed the highest yield (22.60%) and gel strength (365.5 g), respectively. Structural characterization by Fourier transformation infrared spectra, X-ray diffraction, and nuclear magnetic resonance indicated that all gelatins possessed major peaks in the amide region, and diffraction peaks around 22° were basically amorphous. The temperatures for gelling and melting ranged from 20.9 °C to 25.8 °C and 27.34 °C to 30.49 °C. Microstructure revealed loose network with more voids in gelatin from skin pretreated with 0.5 and 0.75 M-NaOH for 6 h, while a highly cross-linked network and less voids were observed in those pretreated with 0.75 M-NaOH for 24 h and 0.5 M-NaOH for 42 h. The results reveal that great potential exists in producing halal gelatin with excellent quality and functionality from camel skin.

Anti‐hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis

“…The extent of hydrolysis for camel gelatin hydrolysates was determined as free amino group content (AN) using the O‐phthaldialdehyde method as previously described by (Ashraf et al ., 2021). In addition, hydrolysis of camel gelatin was further characterised using Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) (Fawale et al ., 2021), and peptide profiling was done by Reverse phase‐ultra performance liquid chromatography (RP‐UPLC) (Mudgil et al ., 2019b). Detailed methodology for each method is described in section S1.2., S1.3.…”

Effect of Ultrasound Pre-Treatment on Extraction and Characterization of Collagen from Bactrian Camel Skin

“…However, few studies have investigated the properties of its collagen, and have mainly focused on gelatin. Nowadays, the highest yield of camel skin gelatin after alkaline treatment is 22.60% [6,10]. However, these conventional extraction methods are usually time consuming, and a considerable amount of insoluble collagen is left behind.…”