Molecular structure of frizzled, a Drosophila tissue polarity gene.

Adler, Paul N.; Vinson, Charles; Park, Woo Jin; Conover, Sharon; Klein, Lauren

doi:10.1093/genetics/126.2.401

Cited by 67 publications

(5 citation statements)

References 27 publications

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“…The Wnt/Ca 2+ pathway regulates cell adhesion and motility (20), and is mediated through release of intracellular Ca 2+ upon Wnt stimulation, and One feature common to these three Wnt signaling pathways is that they all act through the serpertine receptor of the Frizzled family, which consists of at least 10 different members in human. The founding member of the Frizzled family, the frizzled gene of Drosophila, is an essential player in the PCP pathway for establishment of the polarized structure throughout adult cuticle (23,24). Both the frizzled and Frizzled2 genes of Drosophila have been shown to function redundantly as receptors for Wingless in the canonical pathway (25)(26)(27).…”

Section: Overview Of the Wnt Signaling Pathwaysmentioning

confidence: 99%

“…A series of genetic, cell biological, and biochemical studies have provided mounting evidence that members of the Frizzled (Fz) family function as Wnt receptors (25)(26)(27)(41)(42)(43)(44)(45)(46)(47). The Frizzled family consists of at least 10 mammalian members and is named after the first member, the Drosophila tissue polarity gene frizzled (23,24). Structurally the Frizzled proteins are similar to other seven-pass transmembrane proteins, such as G protein-coupled receptors, and have the following features: (i) an extracellular domain that consists of a 120-amino acid, cysteine-rich domain (CRD) characterized by 10 invariantly spaced cysteine residues; (ii) a linker region that shows little sequence similarity among family members; (iii) a highly conserved seven-transmembrane domain; and (iv) a cytoplasmic domain of variable size and little sequence homology among family members.…”

Section: Wnt Receptors -The Frizzled Proteinsmentioning

confidence: 99%

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Specificity of WNT-receptor interactions

Hsieh¹

2004

Front Biosci

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The highly conserved Wnt signaling proteins play critical roles in guiding pattern formation, cell fate decision, and morphogenetic movement during animal development. They bind to the Frizzled family of seven-pass transmembrane proteins and initiate at least three different intracellular signaling pathways, resulting in regulation of gene expression and/or changes in cell behavior. A single transmembrane protein from the low-density-lipoprotein family functions as a co-receptor in the canonical/beta-catenin pathway. The specificity of Wnt signaling depends in part on the affinities between various Wnt-Frizzled pairs. A Wnt-dependent receptor dimerization or clustering step has been hypothesized as the step that initiates the canonical signaling cascade in cells.

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Section: Overview Of the Wnt Signaling Pathwaysmentioning

confidence: 99%

Section: Wnt Receptors -The Frizzled Proteinsmentioning

confidence: 99%

Specificity of WNT-receptor interactions

Hsieh¹

2004

Front Biosci

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“…Although acquisition of cell polarity and cell fate share some genes in common, other genes are clearly unique to each process. For example, unlike dsh, the sple and fz genes are required only for cell polarity since complete loss of function of either gene allows a viable animal with polarity defects but no patterning defects (Gubb and Garcia-Bellido, 1982;Adler et al, 1990). The fz gene encodes a novel protein with seven transmembrane domains reminiscent of G-protein-linked membrane receptors (Vinson et al, 1989) while the molecular nature of the sple gene is unknown.…”

Section: Dsh Is Required Autonomously For Establishment Of Cell Polaritymentioning

confidence: 99%

“…In every region of every body segment, bristles and hairs are arranged in coherent arrays of defined polarity (Piepho, 1955;Gubb and Garcia-Bellido, 1982). Establishment of this polarity appears to involve a cell signaling event requiring at least the dsh, frizzled (fz) and spiny legs (sple) genes (Fahmy and Fahmy, 1959;Gubb and Garcia-Bellido, 1982;Adler et al, 1990;Vinson et al, 1989;Wong and Adler, 1993).…”

Section: Introductionmentioning

confidence: 99%

dishevelled is required during wingless signaling to establish both cell polarity and cell identity

et al. 1994

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The dishevelled gene of Drosophila is required to establish coherent arrays of polarized cells and is also required to establish segments in the embryo. Here, we show that loss of dishevelled function in clones, in double heterozygotes with wingless mutants and in flies bearing a weak dishevelled transgene leads to patterning defects which phenocopy defects observed in wingless mutants alone. Further, polarized cells in all body segments require dishevelled function to establish planar cell polarity, and some wingless alleles and dishevelled; wingless double heterozygotes exhibit bristle polarity defects identical to those seen in dishevelled alone. The requirement for dishevelled in establishing polarity in cell autonomous. The dishevelled gene encodes a novel intracellular protein that shares an amino acid motif with several other proteins that are found associated with cell junctions. Clonal analysis of dishevelled in leg discs provides a unique opportunity to test the hypothesis that the wingless dishevelled interaction species at least one of the circumferential positional values predicted by the polar coordinate model. We propose that dishevelled encodes an intracellular protein required to respond to a wingless signal and that this interaction is essential for establishing both cell polarity and cell identity.

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“…The Fat PCP system consists of two cadherins Fat and Dachsous, a cytoplasmic kinase Four-jointed and an atypical myosin Dachs (Clark et al, 1995; Ishikawa et al, 2008; Mahoney et al, 1991; Mao et al, 2006). The core PCP system is composed of two transmembrane cadherins Frizzled (Fz) and Flamingo or Starry night (Fmi, Stan), the transmembrane protein Strabismus or Van Gogh (Stbm, Vang) and the cytosolic components Dishevelled (Dsh), Prickle (Pk), and Diego (Dgo) (Adler et al, 1990; Devenport, 2014; Feiguin et al, 2001; Gubb and García-Bellido, 1982; Klingensmith et al, 1994; Taylor et al, 1998; Vinson and Adler, 1987; Wolff and Rubin, 1998). Our group has shown that tissue-scale patterns of PCP emerge during larval stages and then are dynamically reoriented during pupal tissue flows by shear stress (Aigouy et al, 2010; Merkel et al, 2014; Sagner et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in theDrosophilapupal wing

Piscitello-Gómez

Gruber

Krishna

et al. 2022

Preprint

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How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing ofDrosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. We showed previously (Etournay et al 2015) that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned. The core planar cell polarity (PCP) pathway influences morphogenetic cell movements in many other contexts, which suggests that it could globally pattern active cellular behaviors during pupal wing morphogenesis. We show here, however, that this is not the case: there is no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation, they do not affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes duringDrosophilapupal wing morphogenesis are independent of one potential chemical guiding cue, core PCP.

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Molecular structure of frizzled, a Drosophila tissue polarity gene.

Cited by 67 publications

References 27 publications

Specificity of WNT-receptor interactions

Specificity of WNT-receptor interactions

dishevelled is required during wingless signaling to establish both cell polarity and cell identity

Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in theDrosophilapupal wing

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